Competitive and allosteric interactions in ligand binding to P-glycoprotein as observed on an immobilized P-glycoprotein liquid chromatographic stationary phase

Molecular Pharmacology

Volumn 59, Issue 1, 2001, Pages 62-68

  Lu, L ^a   Leonessa, F ^a   Clarke, R ^a   Wainer, I W ^a  

^a GEORGETOWN UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CYCLOSPORIN A; DOXORUBICIN; GLYCOPROTEIN P; TRITIUM; VERAPAMIL; VINBLASTINE;

ALLOSTERISM; ARTICLE; DISSOCIATION CONSTANT; DRUG PROTEIN BINDING; LIGAND BINDING; LIQUID CHROMATOGRAPHY; PRIORITY JOURNAL; PROTEIN EXPRESSION;

EID: 0035170479     PISSN: 0026895X     EISSN: None     Source Type: Journal    
DOI: 10.1124/mol.59.1.62     Document Type: Article

References (40)

1. Steroid transport, accumulation and antagonism of P-glycoprotein in multidrug-resistant cells
2. Immobilized membrane vesicle or proteoliposome affinity chromatography. Frontal analysis of interactions of cytochalasin B and D-glucose with the human red cell glucose transporter
3. The functional purification of P-glycoprotein is dependent on maintenance of a lipid-protein interface
4. Cytotoxic drugs and hormones in breast cancer: Interactions at the cellular level
5. Hormone resistance, invasiveness and metastatic potential in human breast cancer
6. Multidrug-resistance gene (P-glycoprotein) is expressed by endothelial cells at blood-brain barrier sites
7. Amino acid substitutions in the sixth transmembrane domain of P-glycoprotein alter multidrug resistance
8. Evidence for two nonidentical drug-interaction sites in the human P-glycoprotein
9. 3H]verapamil radioligand-binding assay
10. A multidrug resistance transporter from human MCF-7 breast cancer cells
11. 3H] vinblastine binding to P-glycoprotein of MCF-7 ADR cells by dexniguldipine
12. P-glycoprotein possesses a 1,4-dihydropyridine-selective acceptor site which is allosterically coupled to a vinca-alkaloid-selective binding site
13. Identification of the multidrug resistance-related P-glycoprotein as a cyclosporin binding protein
14. Competitive and non-competitive inhibition of the multidrug-resistance-associated P-glycoprotein ATPase. Further experimental evidence for a multisite model
15. Biochemistry of multidrug resistance mediated by the multidrug transporter
16. A new in vivo method to study P-glycoprotein transport in tumors and the blood-brain barrier
17. Is the multidrug transporter a flippase?
18. Theoretical aspects of quantitative affinity chromatography: An overview
19. Ligand-receptor interactions: What we can and cannot learn from binding measurements
20. Evaluation of atypical cytochrome P450 kinetics with two-substrate models: Evidence that multiple substrates can simultaneously bind to cytochrome P450
21. P-glycoprotein mediates the efflux of quinidine across the blood-brain barrier
22. MDA435/LCC6 and MDA435/LCC6MDR1: Ascites models of human breast cancer
23. Competitive, non-competitive and cooperative interactions between substrates of P-glycoprotein as measured by its ATPase activity
24. Biology of the multidrug resistance-associated protein, MRP
25. Effect of steroids and verapamil on P-glycoprotein ATPase activity: Progesterone, desoxycorticosterone, corticosterone and verapamil are mutually non-exclusive modulators
26. Multidrug resistance transporter P-glycoprotein has distinct but interacting binding sites for cytotoxic drugs and reversing agents
27. Photosensitized labeling of a functional multidrug transporter in living drug-resistant tumor cells
28. Structure of the multidrug resistance P-glyceprotein to 2.5 nm resolution determined by electron microscopy and image analysis
29. ATPase activity of purified and reconstituted P-glyceprotein from Chinese hamster ovary cells
30. Characterization and functional reconstitution of the multidrug transporter
31. Secondary and tertiary structure changes of reconstituted P-glyceprotein
32. The multidrug resistance-reverser verapamil interferes with cellular P-glyceprotein-mediated pumping of daunorubicin as a non-competing substrate
33. Azidopine noncompetitively interacts with vinblastine and cyclosporin binding to P-glyceprotein in multidrug resistant cells
34. Multidrug resistance in breast cancer: A meta analysis of MDR1/gp170 expression and its possible functional significance
35. P-glycoprotein as the drug efflux pump in primary cultured bovine brain capillary endothelial cells
36. P-glycoprotein and cytookrome P-450 3A inhibition: Dissociation of inhibitory potencies
37. Quantitative characterization of interactions by affinity chromatography in affinity ckromatography
38. Reversal mechanism of multidrug resistance by verapamil: Direct binding of verapamil to P-glyceprotein on specific sites and transport of verapamil outward across the plasma membrane of K562/ADM cells
39. Development of an immobilized P-glycoprotein stationary phase for on-line liquid chromatographic determination of drug-binding affinities
40. Immobilized nicotinic receptor stationary phase for on-line liquid chromatographic determination of drug-receptor affinities