Conserved, N-linked carbohydrates of human immunodeficiency virus type 1 gp41 are largely dispensable for viral replication

Journal of Virology

Volumn 75, Issue 23, 2001, Pages 11426-11436

  Johnson, W E ^a   Sauvron, J M ^a   Desrosiers, R C ^a  

^a HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; CARBOHYDRATE; GLYCOPROTEIN GP 41; MEMBRANE PROTEIN; VIRUS ENVELOPE PROTEIN;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL IMMORTALIZATION; CELL LINE; CODON; CONTROLLED STUDY; GENE CONSTRUCT; GENETIC TRANSFECTION; HUMAN IMMUNODEFICIENCY VIRUS 1; MONONUCLEAR CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN MOTIF; PROTEIN PROCESSING; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SIMIAN IMMUNODEFICIENCY VIRUS; VIRUS ENVELOPE; VIRUS ISOLATION; VIRUS MUTANT; VIRUS REPLICATION;

AMINO ACID SEQUENCE; BASE SEQUENCE; CELL LINE; DNA PRIMERS; GLYCOSYLATION; HIV ENVELOPE PROTEIN GP41; HIV-1; HUMANS; MOLECULAR SEQUENCE DATA; SEQUENCE HOMOLOGY, AMINO ACID; VIRUS REPLICATION;

EID: 0035163480     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.23.11426-11436.2001     Document Type: Article

References (36)

1. Carbohydrate dramatically influences immune reactivity of antisera to viral glycoprotein antigens
2. An N-glycan within the human immunodeficiency virus type 1 gp120 V3 loop affects virus neutralization
3. Specific N-linked and O-linked glycosylation modifications in the envelope V1 domain of simian immunodeficiency virus variants that evolve in the host alter recognition by neutralizing antibodies
4. Core structure of gp41 from the HIV envelope glycoprotein
5. HIV entry and its inhibition
6. Selection for neutralization resistance of the simian/human immunodeficiency virus SHIVSF33A variant in vivo by virtue of sequence changes in the extracellular envelope glycoprotein that modify N-linked glycosylation
7. Deletion of a single N-linked glycosylation site from the transmembrane envelope protein of human immunodeficiency virus type 1 stops cleavage and transport of gp160 preventing env-mediated fusion
8. Role of asparagine-linked glycosylation in human immunodeficiency virus type 1 transmembrane envelope function
9. Characterization of simian-human immunodeficiency virus envelope glycoprotein epitopes recognized by neutralizing antibodies from infected monkeys
10. Functional role of the glycan cluster of the human immunodeficiency virus type 1 transmembrane glycoprotein (gp41) ectodomain
11. gp41. A multifunctional protein involved in HIV entry and pathogenesis
12. Primate models of AIDS
13. + lymphocyte depletion in rhesus monkeys
14. Assembly of asparagine-linked oligosaccharides
15. Mutational analysis of conserved N-linked glycosylation sites of human immunodeficiency virus type 1 gp41
16. Assignment of intrachain disulfide bonds and characterization of potential glycosylation sites of the type 1 recombinant human immunodeficiency virus envelope glycoprotein (gp120) expressed in Chinese hamster ovary cells
17. Crystal structure of the simian immunodeficiency virus (SIV) gp41 core: Conserved helical interactions underlie the broad inhibitory activity of gp41 peptides
18. Neutralization sensitivity of cell culture-passaged simian immunodeficiency virus
19. Complex determinants of macrophage tropism in env of simian immunodeficiency virus
20. Evidence for the cooperation of gp120 amino acids 322 and 448 in SIVmac entry
21. Characterization of infectious molecular clones of simian immunodeficiency virus (SIVmac) and human immunodeficiency virus type 2: Persistent infection of rhesus monkeys with molecularly cloned SIVmac
22. Concepts and principles of glycobiology
23. Alterations in potential sites for glycosylation predominate during evolution of the simian immunodeficiency virus envelope gene in macaques
24. Role of gp41 glycosylation sites in the biological activity of human immunodeficiency virus type 1 envelope glycoprotein
25. An env gene derived from a primary human immunodeficiency virus type 1 isolate confers high in vivo replicative capacity to a chimeric simian/human immunodeficiency virus in rhesus monkeys
26. Identification of replication-competent strains of simian immunodeficiency virus lacking multiple attachment sites for N-linked carbohydrates in variable regions 1 and 2 of the surface envelope protein
27. A role for carbohydrates in immune evasion in AIDS
28. Induction of antibodies against epitopes inaccessible on the HIV type 1 envelope oligomer by immunization with recombinant monomeric glycoprotein 120
29. Rapid selection for an N-linked oligosaccharide by monoclonal antibodies directed against the V3 loop of human immunodeficiency virus type 1
30. Resistance to V3-directed neutralization caused by an N-linked oligosaccharide depends on the quaternary structure of the HIV-1 envelope oligomer
31. Neutralization sensitivity of human immunodeficiency virus type 1 primary isolates to antibodies and CD4-based reagents is independent of coreceptor usage
32. Mutagenesis and synthesis of novel recombinant genes using PCR
33. Atomic structure of the ectodomain from HIV-1 gp41
34. Epitope mapping of two immunodominant domains of gp41, the transmembrane protein of human immunodeficiency virus type 1, using 10 human monoclonal antibodies