Abnormal localisation and hyperclustering of αvβ3 integrins and associated proteins in Src-deficient or tyrphostin A9-treated osteoclasts

Journal of Cell Science

Volumn 114, Issue 1, 2001, Pages 149-160

  Lakkakorpi, P T ^a,b   Nakamura, I ^a   Young, M ^a   Lipfert, L ^a   Rodan, G A ^a   Duong, L T ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

^b UNIVERSITY OF TURKU   (Finland)

Author keywords

v 3 integrin; C Src; Osteoclast; Polarisation

Indexed keywords

ACTIN; ACTIN BINDING PROTEIN; BETA3 INTEGRIN; F ACTIN; FOCAL ADHESION KINASE; LIGAND; PAXILLIN; PROTEIN P130; PROTEIN TYROSINE KINASE; PROTEIN TYROSINE KINASE INHIBITOR; TYRPHOSTIN; VINCULIN;

ANIMAL CELL; ARTICLE; BASEMENT MEMBRANE; BINDING AFFINITY; CELL ADHESION; CELL COMPARTMENTALIZATION; COCULTURE; CONTROLLED STUDY; ENZYME ACTIVITY; LIGAND BINDING; MICROFILAMENT; MOUSE; MOUSE STRAIN; NONHUMAN; ONCOGENE SRC; OSTEOBLAST; OSTEOCLAST; OSTEOLYSIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN PHOSPHORYLATION; PROTEIN STRUCTURE; SPLEEN CELL;

ANIMALS; BONE MATRIX; BONE RESORPTION; CELL ADHESION; CELL MOVEMENT; CELLS, CULTURED; CYTOSKELETAL PROTEINS; FOCAL ADHESION KINASE 2; MICE; MICE, KNOCKOUT; OSTEOCLASTS; PAXILLIN; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN-TYROSINE KINASES; PROTO-ONCOGENE PROTEINS PP60(C-SRC); RECEPTORS, VITRONECTIN; SRC-FAMILY KINASES; TYROSINE; TYRPHOSTINS;

ANIMALIA;

EID: 0035147263     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. c-src expression for osteoclasts to form ruffled borders and resorb bone in mice. J. Clin. Invest. 90, 1622-1627.
2. Bockholt, S. M. and Burrudge, K. (1995). An examination of focal adhesion formation and tyrosine phosphorylation in fibroblasts isolated from src-, fyn-, and yes- mice. Cell Adhesion Commun. 3, 91-100.
3. Chambers, T. J. and Hall, T. J. (1991). Cellular and molecular mechanisms in the regulation and function of osteoclasts. Vitam. Horm. 46, 41-86.
4. Davies, J., Warwick, J., Totty, N., Philp, R., Helfrich, M. and Horton, M. (1989). The osteoclast functional antigen, implicated in the regulation of bone resorption, is biochemically related to the vitronectin receptor. J. Cell Biol. 109, 1817-1826.
5. 3 integrin, and phosphorylated by Src kinase. J. Clin. Invest. 102, 881-892.
6. Felsenfeld, D. P., Schwartzberg, P. L., Venegas, A., Tse, R. and Sheetz, M. P. (1999). Selective regulation of integrin-cytoskeleton interactions by the tyrosine kinase Src. Nature Cell Biol. 1, 200-206.
7. Fincham, V. J. and Frame, M. C. (1998). The catalytic activity of Src is dispensable for translocation to focal adhesions but controls the turnover of these structures during cell motility. EMBO J. 17, 81-92.
8. Suda, T., Nakamura, I., Jimi, E. and Takahashi, N. (1997). Regulation of osteoclast function. J. Bone Miner. Res. 12, 869-879.
9. c-src, preferentially on ruffled border membranes. FEBS Lett. 313, 85-89.
10. Tanaka, S., Amling, M., Neff, L., Peyman, A., Uhlman, E., Levy, J. B. and Baron, R. (1996). c-Cbl is downstream of c-Src in a signaling pathway necessary for bone resorption. Nature 383, 528-531.
11. Wesolowski, G., Duong, L. T., Lakkakorpi, P. T., Nagy, R. M., Tezuka, K., Tanaka, H., Rodan, G. A. and Rodan, S. B. (1995). Isolation and characterization of highly enriched, prefusion mouse osteoclastic cells. Exp. Cell Res. 219, 679-686.