Borate buffer dramatically enhances the activity of poly(ethylene glycol)-α-chymotrypsin complex catalytically active in anhydrous isooctane than conventional phosphate buffer even at low concentration

Biotechnology Letters

Volumn 23, Issue 2, 2001, Pages 125-129

  Murakami, Yoshihiko ^a   Hoshi, Ryotaro ^a   Hirata, Akira ^a  

^a WASEDA UNIVERSITY   (Japan)

Author keywords

chymotrypsin; Enzyme modification; Organic media; Polymer enzyme complex

Indexed keywords

BORIC ACID; BUFFER; CHYMOTRYPSIN A; INORGANIC SALT; MACROGOL 4000; N ACETYLPHENYLALANINE ETHYL ESTER; OCTANE; ORGANIC COMPOUND; PHENYLALANINE DERIVATIVE; PHOSPHATE; PROPANOL; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CHEMICAL COMPOSITION; CONTROLLED STUDY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME MODIFICATION; ESTERIFICATION;

EID: 0035133252     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1010345318275     Document Type: Article

References (18)

1. Organosoluhie enzyme-polymer complexes: A novel type of biocatalyst for nonaqueous media
2. Enzyme function in organic solvents
3. Modification of proteins with polyethylene glycol derivatives
4. Salts dramatically enhance activity of enzymes suspended in organic media
5. Enzymatic catalysis in anhydrous organic solvents
6. Why are enzymes less active in organic solvents than in water?
7. The use of poly(ethylene glycol)-enzymes in nonaqueous enzymology
8. Poly(ethylene glycol)-αchymotrypsin complex catalytically active in anhydrous isooctane
9. Complex between α-chymotrypsin and poly(ethylene glycol) catalytically active in organic media
10. A lipid-coated lipase as an enantioselective ester synthesis catalyst in homogeneous organic solvents
11. Application of novel preparation method for surfactant-protease complexes catalytically active in organic media
12. Complex formation between chymotrypsin and ethyl cellulose as a means to solubilize the enzyme in active form in toluene
13. Polymer-polymer organic solvent two-phase system: A new type of reaction medium for bioorganic synthesis
14. Optimizing the salt-induced activation of enzymes in organic solvent: Effects of lyophilizatin time and water content
15. On the salt-induced activation of lyophilized enzymes in organic solvents: Effect of salt kosmotropicity on enzyme activity
16. A study on the lipid-coating of lipases
17. Dry enzyme-polymer complexes: Stable organosoluble biocatalysts for nonaqueous enzymology
18. Enzymatic catalysis in nonaqueous solvents