Filter-based PNA in situ hybridization for rapid detection, identification and enumeration of specific micro-organisms

Journal of Applied Microbiology

Volumn 90, Issue 2, 2001, Pages 180-189

  Perry O'Keefe, H ^a   Stender, H ^a   Broomer, A ^a   Oliveira, K ^a   Coull, J ^a   Hyldig Nielsen, J J ^a,b  

^a Boston Probes Inc   (United States)

^b NONE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BANDPASS FILTERS; MICROFILTRATION; MICROORGANISMS; RNA;

DETECTION/IDENTIFICATION; FILTER-BASED; FILTRATION TECHNIQUES; IN-SITU HYBRIDIZATION; MEMBRANE FILTRATIONS; MICRO-ORGANISMS; MICROCOLONIES; PEPTIDE NUCLEIC ACID; RAPID DETECTION; SIMULTANEOUS DETECTION;

PROBES;

RIBOSOME RNA;

ARTICLE; CHEMOLUMINESCENCE; GRAM NEGATIVE BACTERIUM; GRAM POSITIVE BACTERIUM; IN SITU HYBRIDIZATION; MEMBRANE FILTER; MICROORGANISM; NONHUMAN; NUCLEIC ACID PROBE; YEAST;

BACTERIA (MICROORGANISMS); NEGIBACTERIA; POSIBACTERIA; PUNCTUM MINUTISSIMUM;

EID: 0035132158     PISSN: 13645072     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2672.2001.01230.x     Document Type: Article

References (31)

1. Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analyzing mixed microbial populations
2. Phylogenetic identification and in situ detection of individual microbial cells without cultivation
3. Chapter 61. Microbial Limits Tests; Chapter 71 Sterility Tests
4. Peptide nucleic acids and their potential applications in biotechnology
5. Phylogenetic stains: Ribosomal RNA-based probes for the identification of single cells
6. PNA hybridizes to complementary oligonucleotides obeying the Watson-Crick hydrogen bonding rules
7. The phylogeny of prokaryotes
8. Differential sensitivity of 16S rRNA targeted oligonucleotide probes used for fluorescence in situ hybridization is a result of ribosomal higher order structure
9. Flow cytometric analysis of the in situ accessibility of Escherichia coli 16S rRNA for fluorescently labeled oligonucleotide probes
10. PNA solubility enhancers
11. The use of alkaline phosphataselabeled oligonucleotides probes as culture confirmation reagents for the identification of commercially important bacteria
12. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences
13. Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method
14. Effects of starvation on physiological activity and chlorine disinfection resistance in Escherichia coli 0157:H7
15. Phylogeny of bacteria beyond the 16S rRNA standard
16. Influence of diluents, media and membrane filters on detection of injured waterborne coliform bacteria
17. Sequence selective recognition of DNA by strand displacement with a thymine-substituted polyamide
18. Quantification
19. Rapid detection of Enterobacteriaceae in urine by fluorescent 16S rRNA in situ hybridization on membrane filters
20. Peptide nucleic acid pre-gel hybridization: An alternative to southern hybridization
21. Use of PNA oligonucleotides for the in situ detection of Escherichia coli in water
22. Monitoring heterotrophic bacteria in potable water
23. Differential amplification of rRNA genes by Polymerase Chain Reaction
24. Detection of mRNA by reverse transcription-PCR as an indicator of viability in Escherichia coli cells
25. Diagnostic applications of PNA oligomers
26. Rapid detection, identification, and enumeration of Pseudomonas aeruginosa in bottled water using peptide nucleic acid probes
27. New automatic rapid detection system of brewing contaminants: Application of Microstar-RMDS-SPS (ATP bioluminescence method) in brewery
28. Development and application of a bioluminescence ATP assay method for rapid detection of coliform bacteria
29. Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotides probe