The use of alkaline phosphatase-labelled oligonucleotide probes as culture confirmation reagents for the identification of commercially important bacteria

Letters in Applied Microbiology

Volumn 27, Issue 2, 1998, Pages 116-120

  Glover, D J ^a   Harris, W J ^b  

^a Natl Collections Indust Mar B   (United Kingdom)

^b UNIVERSITY OF ABERDEEN   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIA; OILS AND FATS; OLIGONUCLEOTIDES; PHOSPHATASES; RNA; SPOILAGE; SULFUR COMPOUNDS;

ALKALINE PHOSPHATASE; CROSS REACTION; FISH AND SHELLFISHES; HELICOBACTER PYLORI; MICRO-ORGANISMS; MYCOBACTERIUM TUBERCULOSIS; OLIGONUCLEOTIDE PROBES; RAPID IDENTIFICATION; SIMPLE++; SULPHATE REDUCING BACTERIA;

PROBES;

ALKALINE PHOSPHATASE;

BACTERIUM; DETECTION METHOD;

ARTICLE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; BIOTECHNOLOGY; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; FISH; FOOD SPOILAGE; HELICOBACTER PYLORI; HYBRIDIZATION; LISTERIA; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; OLIGONUCLEOTIDE PROBE; SHELLFISH;

HELICOBACTER PYLORI; LISTERIA; LISTERIA MONOCYTOGENES; MYCOBACTERIUM TUBERCULOSIS; RENIBACTERIUM SALMONINARUM; STREPTOMYCES; VIBRIO VULNIFICUS;

EID: 0031851929     PISSN: 02668254     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1472-765X.1998.00409.x     Document Type: Article

References (16)

1. Amman, R.I., Binder, B.J., Olson, R.J., Chisholm, S.W., Devereux, R. and Stahl, D.A. (1990) Combination of 16S rRNA-targeted oligonucleotide probes with flow cytometry for analysing mixed microbial populations. Applied and Environmental Microbiology 56, 1919-1925.
2. Anon. (1992) Draft for development DD 211. Method for detection and enumeration of Legionella organisms in water and related materials. London: British Standards Institution.
3. Anon. (1993) BS4285. Microbiological examination for dairy purposes. Section 3.15. Detection of Listeria monocylogenes. London: British Standards Institution.
4. Anon. (1994) Methods for the examination of waters and associated materials. The microbiology of water. Part 1 - Drinking water. Section 7.7. Count of total coliforms and Escherichia coli by membrane filtration. London: HMSO.
5. Aznar, R., Ludwig, W., Amman, R.I. and Schleifer, K.H. (1994) Sequence determination of pathogenic Vibrio species and whole-cell identification of Vibrio vulnificus with rRNA-targeted oligonucleotide probes. International Journal of Systematic Bacteriology 44, 330-337.
6. Carter, J.P., Hsiao, Y.H., Spiro, S. and Richardson, D.J. (1995) Soil and sediment bacteria capable of aerobic nitrate respiration. Applied and Environmental Microbiology 61, 2852-2858.
7. Edwards, L., Rogal, T., Blocker, H., Embde, N. and Bottger, E.C. (1989) Isolation and direct complete nucleotide determination of entire genes. Characterisation of a gene encoding for 16S ribosomal RNA. Nucleic Acids Research 171, 7843-7853.
8. Giovannoni, S.J., Britschig, T.B., Moyer, C. and Field, K.G. (1990) Genetic diversity in Sargasso Sea bacterioplankton. Nature 345, 60-62.
9. Ludwig, W., Kirchof, G., Klugbauer, N. et al. (1993) Complete 23S ribosomal RNA sequences of Gram-positive bacteria with a low DNA G+ C content. Systematic and Applied Microbiology 15, 487-501.
10. Maidak, B.L., Larson, N., McCaughey, J. et al. (1994) The ribosomal database project. Nucleic Acids Research 22, 3485-3487.
11. Mattson, J.G., Gersdorf, H., Jansson, E., Hongslo, T., Gobel, U.B. and Johanssan, K.-E. (1993) Rapid identification of Renibacterium salmoninarum using an oligonucleotide probe complementary to 16S rRNA. Molecular and Cellular Probes 7, 25-33.
12. Morotomi, M., Hoshina, S., Green, P. et al. (1989) Oligonucleotide probe for detection and identification of Campylobacter pylori. Journal of Clinical Microbiology 27, 2652-2655.
13. Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
14. Stackebrandt, E., Witt, D., Kemmerling, C., Kroppendstedt, R. and Liesack, W. (1991) Designation of Streptomycete 16S and 23S rRNA-based target regions for oligonucleotide probes. Applied and Environmental Microbiology 57, 1468-1477.
15. Tevere, J.J., Hewitt, P.L., Dare, A. et al. (1996) Detection of Mycobacterium tuberculosis by PCR amplification with pan-Mycobacterium primer and hybridisation to an M. tuberculosis-specific probe. Journal of Clinical Microbiology 34, 918-923.
16. Wang, R.-F., Cao, W.-W. and Johnson, M.G. (1991) Development of a 16S rRNA-based oligomer probe specific for Listeria monocylogenes. Applied and Environmental Microbiology 57, 3666-3670.