Peptide nucleic acid pre-gel hybridization: An alternative to Southern hybridization

Proceedings of the National Academy of Sciences of the United States of America

Volumn 93, Issue 25, 1996, Pages 14670-14675

  Perry O'Keefe, Heather ^a,b   Yao, Xian Wei ^a   Coull, James M ^a,b   Fuchs, Martin ^a,c   Egholm, Michael ^a  

^a PERKINELMER INC   (United States)

^b Boston Probes Inc   (United States)

^c Active Impulse Systems   (United States)

Author keywords

capillary electrophoresis; chemiluminescence; cystic fibrosis; fluorescence; single base pair discrimination

Indexed keywords

DOUBLE STRANDED DNA; NUCLEIC ACID; PEPTIDE; SINGLE STRANDED DNA;

CHEMOLUMINESCENCE; CONFERENCE PAPER; CONTROLLED STUDY; DNA HYBRIDIZATION; GEL ELECTROPHORESIS; IONIC STRENGTH; NUCLEIC ACID PROBE; PRIORITY JOURNAL; SOUTHERN BLOTTING;

EID: 0029905694     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.93.25.14670     Document Type: Article

References (17)

1. Karp, J. E. & Broder, S. (1996) Nat. Med. 1, 309-320.
2. Dean, M. (1995) Nat. Genet. 9, 103-104.
3. Forrest, S., Cotton, R., Eandegren, U. & Southern, E. (1995) Nat. Genet. 10, 375-376.
4. Southern, E. M. (1975) J. Mol. Biol. 98, 503-517.
5. Egholm, M., Buchardt, O., Nielsen, P. E. & Berg, R. H. (1992) J. Am. Chem. Soc. 114, 1895-1897.
6. Egholm, M., Buchardt, O., Christensen, L., Behrens, C., Freier, S., Driver, D. A., Berg, R. H., Kim, S. K., Norden, B. & Nielsen, P. E. (1993) Nature (London) 365, 566-568.
7. Rose, D. J. (1993) Anal. Chem. 65, 3545-3549.
8. Carlsson, C., Jonsson, M., Nordén, B., Dulay, M. T., Zare, R. N., Noolandi, J., Nielsen, P. E., Tsui, L.-C. & Zielenski, J. (1996) Nature (London) 380, 207.
9. Britten, R. J., Graham, D. E. & Neufeld, B. R. (1974) Methods Enzymol. 29E, 363-405.
10. Riordan, J. R., Rommens, J. M., Kerem, B., Alon, N., Rozmahel, R., Zbyszko, G., Zielenski, J., Lok, S., Plavsic, N., Chou, J., Drumm, M. L., lanuzzi, M. C., Collins, F. M. & Tsui, E. (1989) Science 245, 1066-1073.
11. Kerem, B., Rommens, J. M., Buchanan, J. A., Markiewicz, D., Cox, T., Chakravarti, A., Buchwald, M. & Tsui, E. (1989) Science 245, 1073-1080.
12. Christensen, E. Fitzpatrick, R., Gildea, B., Warren, B. & Coull, J. (1994) Innovation and Perspectives in Solid Phase Synthesis and Complementary Technologies-Biological and Biomedical Applications, SPS Oxford Symposia Book (Mayflower Worldwide, Birmingham, U.K.), p. 149.
13. Mashal, R. D., Koontz, J. & Sklar, J. (1995) Nat. Genet. 9, 177-183.
14. Don, R. H., Cox, P. T., Wainwright, B. J., Baker, K. & Mattick, J. S. (1991) Nucleic Acids Res. 19, 4008.
15. Fung, E. N. & Yeung, E. S. (1995) Anal. Chem. 67, 1913-1919.
16. Handelin, B. & Schuber, A. P. (1994) Current Protocols in Human Molecular Genetics, eds. Dracopoli, N. C., Haines, J. E., Korf, B. R., Moir, D. T., Morton, C. C., Seidman, C. E., Seidman, J. G. & Smith, D. R. (Wiley, New York), pp. 9.4.1-9.4.8.
17. Ørum, H., Nielsen, P. E., Jørgensen, M., Larsson, C., Stanley, C. & Koch, T. (1995) BioTechniques 19, 472-480.