Reversal of RT-PCR inhibition observed in heparinized clinical specimens

BioTechniques

Volumn 23, Issue 1, 1997, Pages 24-28

  Jung, Roman ^a   Lübcke, Cornelia ^a   Wagener, Christoph ^a   Neumaier, Michael ^a  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; DIAGNOSTIC ACCURACY; DIAGNOSTIC VALUE; GENE AMPLIFICATION; HEPARINIZATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

EID: 0343329810     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97231bm03     Document Type: Article

References (10)

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3. Chomczynski, P. and N. Sacchi. 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156-159.
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9. Tsai, M., M. Miyamoto, S.V. Tam, Z.S. Wang and S.J. Galli. 1995. Detection of mouse mast cell-associated protease mRNA. Heparinase treatment greatly improves RT-PCR of tissues containing mast cell heparin. Am. J. Pathol. 146:335-343.
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