A simple method to construct T-vectors using XcmI cassettes amplified by nonspecific PCR

Plasmid

Volumn 45, Issue 1, 2001, Pages 37-40

  Jo, Chulman ^a   Jo, Sangmee Ahn ^a  

^a Korea National Institute of Health   (South Korea)

Author keywords

Nonspecific PCR; T vector; XcmI cassette

Indexed keywords

DNA FRAGMENT; GENE PRODUCT; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; PROTEIN XCMI; THYMIDINE; UNCLASSIFIED DRUG;

ARTICLE; EXPRESSION VECTOR; GENE AMPLIFICATION; GENE CASSETTE; MOLECULAR CLONING; MOLECULAR RECOGNITION; NONHUMAN; POLYMERASE CHAIN REACTION; RECOMBINANT PLASMID; SEQUENCE ANALYSIS;

EID: 0035122606     PISSN: 0147619X     EISSN: None     Source Type: Journal    
DOI: 10.1006/plas.2000.1500     Document Type: Article

References (12)

1. XcmI site-containing vector for direct cloning and in vitro transcription for PCR product
2. XcmI-containing vector for direct cloning of PCR products
3. New vectors for direct cloning of PCR products
4. Novel non-templated nucleotide addition reactions catalyzed by procaryotic and eucaryotic DNA polymerases
5. A vector for direct cloning of PCR products in a double XcmI restriction site offering compatible single 3′-overhanging T residues
6. Direct cloning of polymerase chain reaction products in an XcmI T-vector
7. A simple and efficient method for direct cloning of PCR products using ddT-tailed vectors
8. Construction of new T vectors for direct cloning of PCR products
9. General method for direct cloning of DNA fragments generated by the polymerase chain reaction
10. A universal method for the direct cloning of PCR amplified nucleic acid
11. Cloning unmodified PCR products using engineered XcmI restriction sites in a portable cassette
12. The use of synthetic oligonucleotides as specific hybridization probes in the diagnosis of genetic disorders