XcmI site-containing vector for direct cloning and in vitro transcription of PCR product

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 12, Issue 3, 1999, Pages 281-283

  Arashi Heese, Noriko ^b   Miwa, Masanao ^a   Shibata, Hideo ^b  

^a UNIVERSITY OF TSUKUBA   (Japan)

^b NONE

Author keywords

In situ hybridization; In vitro transcription; PCR; Riboprobe; RT PCR; TA cloning; XcmI

Indexed keywords

RESTRICTION ENDONUCLEASE;

ARTICLE; COSMID VECTOR; DNA SEQUENCE; HYBRIDIZATION; IN SITU HYBRIDIZATION; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA PROBE; SEQUENCE ANALYSIS; TRANSCRIPTION REGULATION;

EID: 0033426926     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/mb:12:3:281     Document Type: Article

References (5)

1. 1. Chuang, S. E., Wang, K. C., and Cheng, A. L. (1995) Single-step direct cloning of PCR products. Trends in Genet. 11, 7-8.
2. 2. de Vries, E. (1998) pUCPCRI. A vector for direct cloning of PCR products in a double XcmI restriction site offering compatible single 3′-overhanging T residues. Mol. Biotechnol. 10, 273,274.
3. 3. Borovkov, A. Y. and Rivkin, M. I. (1997) XcmI-containing vector for direct cloning of PCR products. Biotechniques 22, 812-814.
4. 4. Testori, A. and Sollitti, P. (1997) Cloning unmodified PCR products using engineered XcmI restriction sites in a portable cassette. Methods Mol. Biol. 67, 89-100.
5. 5. Ido, E. and Hayami, M. (1997) Construction of T-tailed vectors derived from a pUC plasmid: a rapid system for direct cloning of unmodified PCR products. Biosci. Biotechnol. Biochem. 61, 1766,1767.