Paired cloning of the T cell receptor α and β genes from a single T cell without the establishment of a T cell clone

Clinical and Experimental Immunology

Volumn 123, Issue 2, 2001, Pages 340-345

  Kurokawa, M ^a   Tong, J ^a   Matsui, T ^a   Masuko Hongo, K ^a   Yabe, T ^a,b   Nishioka, K ^a   Yamamoto, K ^a,b   Kato, T ^a  

^a ST MARIANNA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

^b UNIVERSITY OF TOKYO   (Japan)

Author keywords

Single cell PCR; Systemic lupus erythematosus; T cell; T cell receptor

Indexed keywords

COMPLEMENTARY DNA; MAJOR HISTOCOMPATIBILITY ANTIGEN; RECOMBINANT RECEPTOR; T LYMPHOCYTE RECEPTOR ALPHA CHAIN; T LYMPHOCYTE RECEPTOR BETA CHAIN; T LYMPHOCYTE RECEPTOR DELTA CHAIN; T LYMPHOCYTE RECEPTOR GAMMA CHAIN;

ANTIGEN RECOGNITION; ANTIGEN SPECIFICITY; ARTICLE; CELL SELECTION; CLONE; CONTROLLED STUDY; GENE SEQUENCE; HUMAN; HUMAN CELL; MOLECULAR CLONING; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; T LYMPHOCYTE;

CELL DIFFERENTIATION; CLONE CELLS; CLONING, MOLECULAR; HUMANS; POLYMERASE CHAIN REACTION; RECEPTORS, ANTIGEN, T-CELL, ALPHA-BETA; T-LYMPHOCYTES;

EID: 0035114741     PISSN: 00099104     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2249.2001.01437.x     Document Type: Article

References (17)

1. Accumulation of multiple T cell clonotypes in the synovial lesions of patients with rheumatoid arthritis revealed by a novel clonality analysis
2. Correlation of clonal T cell expansion with disease activity in systemic lupus erythematosus
3. Clonotype analysis of T cells in patients with autoimmune and viral hepatitis
4. Frequency of clonally expanding T cells evaluated by PCR from a single cell
5. Interaction of Staphylococcus aureus toxin 'superantigens' with human T cells
6. Human T-cell receptor variable gene segment families
7. Mammalian expression of single chain variable region fragments dimerized by Fc regions
8. Highly efficient recovery of functional single-chain Fv fragments from inclusion bodies overexpressed in Escherichia coli by controlled introduction of oxidizing reagent - Application to a human single-chain Fv fragment
9. Characterization of T cell receptor single-chain Fv fragments secreted by myeloma cells
10. Binding of soluble natural ligands to a soluble human T-cell receptor fragment produced in Escherichia coli
11. Construction of a functional disulfide-stabilized TCR Fv indicates that antibody and TCR Fv frameworks are very similar in structure
12. A three-domain T cell receptor is biologically active and specifically stains cell surface MHC/peptide complexes
13. Whole genome amplification from a single cell: Implications for genetic analysis
14. Preimplantation single-cell analysis of multiple genetic loci by whole-genome amplification
15. Detailed chromosomal and molecular genetic analysis of single cells by whole genome amplification and comparative genomic hybridisation
16. Induction and exhaustion of lymphocytic choriomeningitis virus-specific cytotoxic T lymphocytes visualized using soluble tetrameric major histocompatibility complex class I-peptide complexes
17. Direct analysis of viral-specific CD8+ T cells with soluble HLA-AS/Tax11-19 tetramer complexes in patients with human T cell lymphotrophic virus-associated myelopathy