Molecular cloning and characterization of WdPKS1, a gene involved in dihydroxynaphthalene melanin biosynthesis and virulence in Wangiella (Exophiala) dermatitidis

Infection and Immunity

Volumn 69, Issue 3, 2001, Pages 1781-1794

  Feng, B ^a   Wang, X ^a   Hauser, M ^a   Kaufmann, S ^a   Jentsch, S ^a   Haase, G ^a   Becker, J M ^a   Szaniszlo, P J ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

1,8 DIHYDROXYNAPHTHALENE MELANIN; 3 OXOACYL ACYL CARRIER PROTEIN SYNTHASE; FUNGAL PROTEIN; GENE PRODUCT; MELANIN; NAPHTHALENE DERIVATIVE; PROTEIN WDPKS1; UNCLASSIFIED DRUG;

ALBINISM; ANIMAL EXPERIMENT; ARTICLE; CELL WALL; CONTROLLED STUDY; EXON; FUNGUS; GENE AMPLIFICATION; GENETIC ANALYSIS; MALE; MELANOGENESIS; MOLECULAR CLONING; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PHAEOHYPHOMYCOSIS; PHENOTYPE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DOMAIN; SEQUENCE ANALYSIS; VIRULENCE; WANGIELLA DERMATIDIS;

AMINO ACID SEQUENCE; ANIMALS; CLONING, MOLECULAR; EXOPHIALA; GENE TARGETING; GENES, FUNGAL; HUMANS; MELANINS; MICE; MOLECULAR SEQUENCE DATA; MULTIENZYME COMPLEXES; MYCOSES; NAPHTHOLS; NEUTROPHILS; PHAGOCYTOSIS; POLYMERASE CHAIN REACTION; RESPIRATORY BURST; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; VIRULENCE;

EID: 0035114569     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/IAI.69.3.1781-1794.2001     Document Type: Article

References (58)

1. Optical measurements of invasive forces exerted by appressoria of a plant pathogenic fungus
2. The multifunctional 6-methylsalicylic acid synthase gene of Penicillium patulum: Its gene structure relative to that of other polyketide synthases
3. Use of mutants to establish (+)-scytalone as an intermediate in melanin biosynthesis by Verticillium dahliae
4. Invasive hyphal growth in Wangiella dermatitidis is induced by stab inoculation and shows dependence upon melanin biosynthesis
5. Improved vectors for selecting resistance to hygromycin
6. The Aspergillus parasiticus polyketide synthase gene pksA, a homolog of Aspergillus nidulans wA, is required for aflatoxin B1 biosynthesis
7. Pigment production by Cryptococcus neoformans and other Cryptococcus species from aminophenols and diaminobenzenes
8. Genetic analysis of melanin-deficient, non-pathogenic mutants of Magnaporthe grisea
9. Evidence for two cell division cycle (CDC) genes that govern yeast bud emergence in the pathogenic fungus Wangiella dermatitidis
10. Melanin as a virulence factor in dematiaceous pathogenic fungi
11. Pleoanamorphic life cycle of Exophiala (Wangiella) dermatitidis
12. Pathogenicity and virulence of wild-type and melanin-deficient Wangiella dermatitidis
13. - mutants of Wangiella dermatitidis in mice: Evaluation of multiple mouse and fungal strains
14. Melanized and non-melanized multicellular form mutants of Wangiella dermatitidis in mice: Mortality and histopathology studies
15. Dihydroxynaphthalene (DHN) melanin and its relationship with virulence in the early stages of phaeohyphomycosis
16. Heterologous expression and product identification of Colletotrichum lagenarium polyketide synthase encoded by the PKS1 gene involved in melanin biosynthesis
17. Cloning of the polyketide synthase gene atX from Aspergillus terreus and its identification as the 6-methylsalicylic acid synthase gene by heterologous expression
18. Enzymatic synthesis of 1.3,6.8-tetrahydroxynaphthalene solely from malonyl coenzyme A by a fungal iterative type I polyketide synthase PKS1
19. Pentaketide metabolites of melanin synthesis in the dematiaceous fungus Wangiella dermatitidis
20. Molecular genetics of polyketides and its comparison to fatty acid biosynthesis
21. Antioxidant function of melanin in black fungi
22. Gene cluster involved in melanin biosynthesis of the filamentous fungus Alternaria alternata
23. Melanin biosynthesis as a prerequisite for penetration by appressoria of Colletotrichum lagenarium: Site of inhibition by melanin-inhibiting fungicides and their action on appressoria
24. Molecular genetics analysis of melanin biosynthetic genes essential for appressorium function in Colletotrichum lagenarium
25. Cloning and structural analysis of the melanin biosynthesis gene SCD1 encoding scytalone dehydratase in Colletotrichum lagenarium
26. Free CoA as activator of 6-hydroxymellein synthase, a multifunctional polyketide biosynthetic enzyme in carrot
27. Encapsulation and melanin in Cryptococcus neoformans
28. Identification of a polyketide synthase gene (pksP) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence
29. Crystal structure of scytalone dehydratase: The disease determinant of a rice pathogen. Magnaporthe grisea
30. Laccase and tyrosinase oxidation of spot test reagents
31. Clinical and mycological spectra of Wangiella dermatitidis infections
32. Developments in hyalohyphomycosis and phaeohyphomycosis
33. The developmentally regulated Aspergillus nidulans wA gene encodes a polypeptide homologous to polyketide and fatty acid synthases
34. β-Glucosylated proteins in the cell wall of the black yeast Exophiala (Wangiella) dermatitidis
35. A versatile shuttle cosmid vector for the efficient construction of genomic libraries and for the cloning of fungal genes
36. Cloning and characterization of a melanin biosynthetic THR reductase gene essential for appressorial penetration of Colletotrichum lagenarium
37. Measuring resistance to phagocytosis of group A and G streptococci: Comparison of direct bactericidal assay and flow cytometry
38. Effect of melanin and carotenoids of Exophiala (Wangiella) dermatitidis on phagocytosis, oxidative burst, and killing by human neutrophils
39. Clues about chromoblastomycotic and other dematiaceous fungal pathogens based on Wangiella as a model
40. Purification of a melanin biosynthetic enzyme converting scytalone to 1,3,8-trihydroxynaphthalene from Cochliobolus miyabeanus
41. Structural analysis of PKS1, a polyketide synthase gene involved in melanin biosynthesis in Colletotrichum lagenarium
42. Evidence for pentaketide melanin biosynthesis in dematiaceous human pathogenic fungi
43. The developmentally regulated alb1 gene of Aspergillus fumigatus: Its role in modulation of conidial morphology and virulence
44. Polyhydroxynapthalene reductase involved in melanin biosynthesis in Magnaporthe grisea
45. WdCHS3, a gene that encodes a class III chitin synthase in Wangiella (Exophiala) dermatitidis, is expressed differentially under stress conditions
46. WdChs4p, a homolog of chitin synthase 3 in Saccharomyces cerevisiae, alone cannot support growth of Wangiella (Exophiala) dermatitidis at the temperature of infection
47. Product identification of polyketide synthase coded by Aspergillus nidulans wA gene
48. Melanin biosynthesis in Verticillium dahliae: Dehydration and reduction reactions in cell-free homogenates
49. Melanins and their importance in pathogenic fungi
50. Melanin biosynthesis and the metabolism of flaviolin and 2-hydroxyjuglone in Wangiella dermatitidis
51. Tricyclazole, pyroquilon, tetrachlorophthalide, PCBA, coumarine and related compounds inhibit melanization and epidermal penetration by Pyricularia oryzae
52. A color-selectable and site-specific integrative transformation system for gene expression studies in the dematiaceous fungus Wangiella (Exophiala) dermatitidis
53. A cosmid for selecting genes by complementation in Aspergillus nidulans: Selection of the developmentally regulated yA locus
54. Sterigmatocystin biosynthesis in Aspergillus nidulans requires a novel type I polyketide synthase
55. Cloning and use of they WdURA5 gene as a hisG cassette selection marker for potentially disrupting multiple genes in Wangiella dermatitidis