An improved method for conjugating monoclonal antibodies with N-hydroxysulfosuccinimidyl DOTA

Bioconjugate Chemistry

Volumn 12, Issue 2, 2001, Pages 320-324

  Lewis, M R ^a   Kao, J Y ^a   Anderson, A L J ^a   Shively, J E ^a   Raubitschek, A ^a  

^a CITY OF HOPE NATIONAL MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SURFACE PLASMON RESONANCE;

CARCINOEMBRYONIC ANTIGEN; CROSS LINKING; HIGH SPECIFIC ACTIVITIES; LARGE SCALE PRODUCTIONS; PH BUFFERS; PH CONDITION; SIMPLE++; TEMPERATURE CONDITIONS; TETRAACETIC ACID; WATERSOLUBLE;

MONOCLONAL ANTIBODIES;

ANIMALIA; MURINAE;

EID: 0035083298     PISSN: 10431802     EISSN: None     Source Type: Journal    
DOI: 10.1021/bc0000886     Document Type: Article

References (26)

1. Monoclonal antibodies in diagnosis and therapy
2. Vehicles, chelators, and radionuclides: Choosing the "building blocks" of an effective therapeutic radioimmunoconjugate
3. Chelating agents for the binding of metal ions to antibodies
4. Newer approaches to the radiolabeling of monoclonal antibodies by use of metal chelates
5. Radiolabeling of monoclonal antibodies with metal chelates
6. Nature of the bifunctional chelating agent used for radioimmunotherapy with yttrium-90 monoclonal antibodies: Critical factors in determining in vivo survival and organ toxicity
7. Coordination of lanthanides by two polyamino polycarboxylic macrocycles: Formation of highly stable lanthanide complexes
8. Thermodynamic study of lanthanide complexes of 1,4,7-triazacyclononane-N,N′,N″-triacetic acid and 1,4,7,10-tetraazacyclododecane-N,N′,N″,N‴-tetraacetic Acid
9. Kinetics of interaction of metal ions with two tetraaza tetraacetate macrocycles
10. A kinetic investigation of the lanthanide DOTA chelates. Stability and rates of formation and of dissociation of a macrocyclic gadolinium(III) polyaza polycarboxylic MRI contrast agent
11. Yttrium-90-labeled monoclonal antibody for therapy: Labeling by a new macrocyclic bifunctional chelating agent
12. 90Y from cyclic and acyclic ligand-antibody conjugates
13. Pharmacokinetics of chimeric L6 conjugated to indium-111 and yttrium-90-DOTA-peptide in tumor-bearing mice
14. 90Y-labeled DOTA and maleimidocysteineamido-DOTA conjugated to chimeric anti-carcinoembryonic antigen antibody in xenograft-bearing nude mice: Comparison of stable and chemically labile linker systems
15. A facile, water-soluble method for modification of proteins with DOTA. Use of elevated temperature and optimized pH to achieve high specific activity and high chelate stability in radiolabeled immunoconjugates
16. Cloning of the genes for T84.66, an antibody that has a high specificity and affinity for carcinoembryonic antigen, and expression of chimeric human/mouse T84.66 genes in myeloma and Chinese hamster ovary cells
17. Conjugation of antibodies with bifunctional chelating agents: Isothiocyanate and bromoacetamide reagents, methods of analysis, and subsequent addition of metal ions
18. Isolation and characterization of carcinoembryonic antigen
19. Characterization of carcinoembryonic antigen fractionated by concanavalin A chromatography
20. Stability of water-soluble carbodiimides in aqueous solution
21. Mechanism of amide formation by carbodiimide for bioconjugation in aqueous media
22. Reactions of N-hydroxysulfosuccinimide active esters
23. Zero-length crosslinking procedure with the use of active esters
24. A method for the high efficiency of water-soluble carbodiimide-mediated amidation
25. Enhancement by N-hydroxysulfosuccinimide of water-soluble carbodiimide-mediated coupling reactions
26. Effect of the extent of chelate substitution on the immunoreactivity and biodistribution of 2IT-BAT-Lym-1 immunoconjugates