Evaluation of interactions of human cytomegalovirus immediate-early IE2 regulatory protein with small ubiquitin-like modifiers and their conjugation enzyme Ubc9

Journal of Virology

Volumn 75, Issue 8, 2001, Pages 3859-3872

  Ahn, J H ^a   Xu, Y ^a   Jang, W J ^a   Matunis, M J ^a   Hayward, G S ^a  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; LYSINE; PHOSPHOPROTEIN; PROTEIN SUMO 2; PROTEIN SUMO 3; REGULATOR PROTEIN; SMALL UBIQUITIN LIKE MODIFIER CONJUGATING ENZYME; SUMO 1 PROTEIN; UNCLASSIFIED DRUG; VIRUS PROTEIN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CELLULAR DISTRIBUTION; CONTROLLED STUDY; CYTOMEGALIC INCLUSION BODY DISEASE; CYTOMEGALOVIRUS; DNA REPLICATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN PROTEIN INTERACTION; TUMOR PROMOTION; VIRUS RECOMBINATION;

AMINO ACID SEQUENCE; ANIMALS; B-LYMPHOCYTES; BINDING SITES; CELL LINE; CYTOMEGALOVIRUS; DNA, VIRAL; HUMANS; IMMEDIATE-EARLY PROTEINS; LIGASES; MEMBRANE GLYCOPROTEINS; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN BINDING; PROTEIN PROCESSING, POST-TRANSLATIONAL; SEQUENCE ALIGNMENT; SMALL UBIQUITIN-RELATED MODIFIER PROTEINS; SUMO-1 PROTEIN; TIME FACTORS; TRANS-ACTIVATION (GENETICS); TRANS-ACTIVATORS; TWO-HYBRID SYSTEM TECHNIQUES; UBIQUITIN-CONJUGATING ENZYMES; UBIQUITINS; VIRAL ENVELOPE PROTEINS; VIRAL PROTEINS; VIRUS REPLICATION;

EID: 0035082279     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.75.8.3859-3872.2001     Document Type: Article

References (64)

1. Disruption of PML subnuclear domains by the acidic IE1 protein of human cytomegalovirus is mediated through interaction with PML and may modulate a RING finger-dependent cryptic transactivator function of PML
2. Evaluation and mapping of the DNA binding and oligomerization domains of the IE2 regulatory protein of human cytomegalovirus using yeast one and two hybrid interaction assays
3. Disruption of PML-associated nuclear bodies by IE1 correlates with efficient early stages of viral gene expression and DNA replication in human cytomegalovirus infection
4. The major immediate-early proteins IE1 and IE2 of human cytomegalovirus colocalize with and disrupt PML-associated nuclear bodies at very early times in infected permissive cells
5. The human cytomegalovirus IE2 and UL112-113 proteins accumulate in viral DNA replication compartments that initiate from the periphery of promyelocytic leukemia protein-associated nuclear bodies (PODs or ND10)
6. PIC 1, a novel ubiquitin-like protein which interacts with the PML component of a multiprotein complex that is disrupted in acute promyelocytic leukaemia
7. The cyclin E promoter is activated by human cytomegalovirus 86-kDa immediate early protein
8. Cytomegalovirus
9. SUMO-1 modification of Mdm2 prevents its self-ubiquitination and increases Mdm2 ability to ubiquitinate p53
10. The human cytomegalovirus 86K immediate early (IE) 2 protein requires the basic region of the TATA-box binding protein (TBP) for binding, and interacts with TBP and transcription factor TFIIB via regions of IE2 required for transcriptional regulation
11. Identification and mapping of dimerization and DNA-binding domains in the C terminus of the IE2 regulatory protein of human cytomegalovirus
12. SUMO-1 modification of IκBα inhibits NF-κB activation
13. Ubch9 conjugates SUMO but not ubiquitin
14. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit
15. Preferential interaction of sentrin with a ubiquitin-conjugating enzyme, Ubc9
16. Activation of p53 by conjugation to the ubiquitin-like protein SUMO-1
17. Defective growth correlates with reduced accumulation of a viral DNA replication protein after low-multiplicity infection by a human cytomegalovirus ie1 mutant
18. Functional interaction between the HCMV IE2 transactivator and the retinoblastoma protein
19. The human cytomegalovirus 80-kilodalton but not the 72-kilodalton immediate-early protein transactivates heterologous promoters in a TATA box-dependent mechanism and interacts directly with TFIID
20. Covalent modification of the transactivator protein IE2-p86 of human cytomegalovirus by conjugation to the ubiquitin-homologous proteins SUMO-1 and hSMT3b
21. The periphery of nuclear domain 10 (ND10) as site of DNA virus deposition
22. PML is critical for ND10 formation and recruits the PML-interacting protein Daxx to this nuclear structure when modified by SUMO-1
23. Human cytomegalovirus immediate early interaction with host nuclear structures: Definition of an immediate transcript environment
24. Cytomegalovirus infection induces high levels of cyclins, phosphorylated Rb, and p53, leading to cell cycle arrest
25. Cell cycle-regulated attachment of the ubiquitin-related protein SUMO to the yeast septins
26. Ubc9p is the conjugating enzyme for the ubiquitin-like protein Smt3p
27. SUMO-1: Ubiquitin gains weight
28. Direct interaction of the human cytomegalovirus IE86 protein with the cis repression signal does not preclude TBP from binding to the TATA box
29. Human cytomegalovirus IE86 protein interacts with promoter-bound TATA-binding protein via a specific region distinct from the autorepression domain
30. Differences in cell-type-specific blocks to immediate early gene expression and DNA replication of human, simian and murine cytomegalovirus
31. Expression of the acidic nuclear immediate-early protein (IE1) of human cytomegalovirus in stable cell lines and its preferential association with metaphase chromosomes
32. Structure and function in the nucleus
33. Functional interaction between the human cytomegalovirus 86-kilodalton IE2 protein and the cellular transcription factor CREB
34. Sequestration and inhibition of Daxx-mediated transcriptional repression by PML
35. A cis-acting element in the major immediate-early promoter of human cytomegalovirus is required for negative regulation by IE2
36. Human cytomegalovirus contains a tegument protein that enhances transcription from promoters with upstream ATF and AP-1. cis-acting elements
37. Formation of herpes simplex virus type 1 replication compartments by transfection: Requirements and localization to nuclear domain 10
38. A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2
39. A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex
40. SUMO-1 modification and its role in targeting the Ran GTPase-activating protein, RanGAP1, to the nuclear pore complex
41. Nuclear domain 10 as preexisting potential replication start sites of herpes simplex virus type-1
42. Dysregulation of cyclin E gene expression in human cytomegalovirus-infected cells requires viral early gene expression and is associated with changes in the Rb-related protein p130
43. Covalent modification of p73alpha by SUMO-1. Two-hybrid screening with p73 identifies novel SUMO-l-interacting proteins and a SUMO-1 interaction motif
44. Cytomegalovirus and their replication
45. c-Jun and p53 activity is modulated by SUMO-1 modification
46. Conjugation with the ubiquitin-related modifier SUMO-1 regulates the partitioning of PML within the nucleus
47. The IE2 gene products of human cytomegalovirus specifically down-regulate expression from the major immediate-early promoter through a target sequence located near the cap site
48. The functionally active IE2 immediate-early regulatory protein of human cytomegalovirus is an 80-kilodalton polypeptide that contains two distinct activator domains and a duplicated nuclear localization signal
49. trans-Activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus
50. SUMO-1 modification activates the transcriptional response of p53
51. Functional heterogeneity of small ubiquitin-related protein modifiers SUMO-1 versus SUMO-2/3
52. SUMO-1: Wrestling with a new ubiquitin-related modifier
53. The human cytomegalovirus IE2 86-kilodalton protein interacts with an early gene promoter via site-specific DNA binding and protein-protein associations
54. Transactivation by the human cytomegalovirus IE2 86-kilodalton protein requires a domain that binds to both the TATA box-binding protein and the retinoblastoma protein
55. Insertion and deletion mutagenesis of the human cytomegalovirus genome
56. Potential role of human cytomegalovirus and p53 interaction in coronary restenosis
57. Multiple spliced and unspliced transcripts from human cytomegalovirus immediate-early region 2 and evidence for a common initiation site within immediate-early region 1
58. The nuclear dot protein spl00, characterization of domains necessary for dimerization, subcellular localization, and modification by small ubiquitin-like modifiers
59. Organization and expression of the immediate early genes of human cytomegalovirus
60. Binding of the human cytomegalovirus 80-kD immediate-early protein (IE2) to minor groove A/T-rich sequences bounded by CG dinucleotides is regulated by protein oligomerization and phosphorylation
61. Cytomegalovirus IE2 protein stimulates interleukin-1β gene transcription via tethering to Spi-1/PU.1
62. The IE2 regulatory protein of human cytomegalovirus induces expression of the human transforming growth factor β1 gene through an Egr-1 binding site
63. Role of SUMO-1-modified PML in nuclear body formation