Evaluation and mapping of the DNA binding and oligomerization domains of the IE2, regulatory protein of human cytomegalovirus using yeast one and two hybrid interaction assays

Gene

Volumn 210, Issue 1, 1998, Pages 25-36

  Ahn, Jin Hyun ^a   Chiou, Chuang Jiun ^b   Hayward, Gary S ^a,b  

^a Dept Pharmacol Molec Sci J   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Autoregulation; Cis repression signal; Domain mapping; HCMV; Transactivation

Indexed keywords

DNA; DNA BINDING PROTEIN; HYBRID PROTEIN; IMMEDIATE EARLY PROTEIN; REGULATOR PROTEIN; VIRUS PROTEIN;

ARTICLE; ASSAY; AUTOREGULATION; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DIMERIZATION; DNA TRANSFECTION; HUMAN CYTOMEGALOVIRUS; NONHUMAN; OLIGOMERIZATION; POINT MUTATION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN DNA BINDING; PROTEIN DOMAIN; TRANSACTIVATION; VERO CELL; YEAST;

BACTERIA (MICROORGANISMS); HUMAN HERPESVIRUS 5; MAMMALIA;

EID: 0032571556     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(98)00056-0     Document Type: Article

References (34)

1. Ahn J.-H., Hayward G.S. The major immediate-early proteins IE1 and IE2 of human cytomegalovirus co-localize with and disrupt PML-associated nuclear bodies at very early times in infected permissive cells. J. Virol. 71:1997;4599-4613.
2. Caswell R., Hagemeier C., Chiou C.-J., Haywrd G.S., Kouzarides T., Sinclair J. The human cytomegalovirus 86K immediate early (IE) 2 protein requires the basic region of the TATA-box binding protein (TBP) for binding and interacts with TBP and trascription factor TFIIB via regions of IE2 required for transcriptional regulation. J. Gen. Virol. 74:1993;2691-2698.
3. Chang Y.-N., Jeang J.-T., Lietman T., Hayward G.S. Structural organization of the spliced immediate-early gene complex that encodes the major acidic nuclear (IE1) and trans-activator (IE2) proteins of African green monkey cytomegalovirus. J. Biomed. Sci. 2:1995;105-130.
4. Cherrington J.M., Khoury E.L., Mocarski E.S. Human cytomegalovirus 'ie'2 negatively regulates alpha gene expression via a short target sequence near the transcription start site. J. Virol. 65:1991;887-896.
5. Chiou C.-J., Zong J., Waheed I., Hayward G.S. Identification and mapping of dimerization and DNA-binding domains in the C-terminus of the IE2 regulatory protein of human cytomegalovirus. J. Virol. 67:1993;6201-6214.
6. Chiou, C.-J., Ahn, J.-H., Hayward, G.S., in preparation. The central modulator domain of the human cytomegalovirus IE2 protein interacts with the C-terminus of p53 and the B/C pocket of Rb in vitro and is required for both the transactivator and repressor functions of the protein.
7. Durfee T., Becherer K., Chen P.L., Yeh S.H., Yang U., Kilburn A., Lee W.E., Elledge S.J. The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit. Genes Dev. 7:1993;555-569.
8. Furnari B., Poma E., Kowalik T.F., Huong S.-M., Huang E.-S. Human cytomegalovirus immediately-eary 2 protein interacts with itself and with several novel cellular proteins. J. Virol. 67:1993;4981-4991.
9. Guarente L., Ptashne M. Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA. 78:1981;2199-2203.
10. Hagemeier C., Walker S., Caswell R., Kouzarides T., Sinclair J. The human cytomegalovirus 80-kilodalton but not the 72-kilodalton immediate-early protein transactivates heterologous promoters in a TATA box-dependent mechanim and interacts directly with TFIID. J. Virol. 66:1992;4452-4456.
11. Hagemeier C., Caswell R., Hayhurst G., Sinclair J., Kouzarides T. Functional interaction between the HCMV IE2 transactivator and the retinoblastoma protein. EMBO J. 13:1994;2897-2903.
12. Hermiston T.W., Malone C.L., Witte P.R., Stinski M.F. Identification and characterization of the human cytomegalovirus immediate-early region 2 gene that stimulates gene expression from an inducible promoter. J. Virol. 61:1987;3214-3221.
13. Jupp R., Hoffmann S., Depto A., Stenberg R.M., Ghazal P., Nelson J.A. Direct interaction of the human cytomegalovirus IE86 protein with the cis repression signal does not preclude TBP from binding to the TATA box. J. Virol. 67:1993;5595-5604.
14. Jupp R., Hoffmann S., Stenberg R.M., Nelson J.A., Ghazal P. Human cytomegalovirus IE86 protein interacts with promoter-bound TATA-binding protein via a specific region distinct from the autorepression domain. J. Virol. 67:1993;7539-7546.
15. Lang D., Stamminger T. The 86-kilodalton IE-2 protein of human cytomegalovirus is a sequence-specific DNA-binding protein that interacts directly with the negative autoregulatory response element located near the cap site of the IE-1,2 enhancer-promoter. J. Virol. 67:1993;323-331.
16. Lang D., Stamminger T. Minor groove contacts are essential for an interaction of the human cytomegalovirus IE2 protein with its DNA target. Nucleic Acids Res. 22:1994;3331-3338.
17. Lang D., Gebert S., Arlt H., Stamminger T. Functional interaction between the human cytomegalovirus 86-kilodalton IE2 protein and the cellular transcription factor CREB. J. Virol. 69:1995;6030-6037.
18. Liu B., Hermiston T.W., Stinski M.F. A 'cis'-acting element in the major immediate-early (IE) promoter of human cytomegalovirus is required for negative regulation by IE2. J. Virol. 65:1991;897-903.
19. Macias M.P., Stinski M.F. An in vitro system for human cytomegalovirus immediate early 2 protein (IE2)-mediated site-dependent repression of transcription and direct binding of IE2 to the major immediate early promoter. Proc. Natl. Acad. Sci. USA. 90:1993;707-711.
20. Pizzorno M.C., O'Hare P., Sha L., LaFemina R.L., Hayward G.S. Trans-activation and autoregulation of gene expression by the immediate-early region 2 gene products of human cytomegalovirus. J. Virol. 62:1988;1167-1179.
21. Pizzorno M.C., Hayward G.S. The IE2 gene products of human cytomegalovirus specifically down-regulate expression from the major immediate-early promoter through a target sequence located near the cap site. J. Virol. 64:1990;6154-6165.
22. Pizzorno M.C., Mullen M.-A., Chang Y.-N., Haywrd G.S. The functionally active IE2 immediately-early regulatory protein of human cytomegalovirus is an 80-kilodalton polypeptide that contains two distinct activator domains and a duplicated nuclear localization signal. J. Virol. 65:1991;3839-3852.
23. Rose, M.D., Winston, F., Hieter, P., 1990. Methods in Yeast Genetics. A Laboratory Course Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
24. Sommer M.H., Scully A.L., Spector D.H. Transactivation by human cytomegalovirus IE 86-kilodalton protein requires a domain that binds to both the TATA box-binding protein and the retinoblastoma protein. J. Virol. 68:1994;6223-6231.
25. Spaete R.R., Stinski M.F. Regulation of cytomegalovirus gene expression; α and β promoters are trans-activated by viral functions in permissive human fibroblast. J. Virol. 56:1985;135-143.
26. Spector D.J., Tevethia M.J. Identification of a human cytomegalovirus virus DNA segment that complements an adenovirus 5 immediate-early mutant. Virology. 151:1986;329-338.
27. Spector D.J., Tevethhia M.J. Protein-protein interactions between human cytomegalovirus IE2-580aa and pUL84 in lytically infected cells. J. Virol. 68:1994;7549-7553.
28. Speir E., Modali R., Huang E.-S., Leon M.B., Shawl F., Finkel T., Epstein E.S. Potential role of human cytomegalovirus and p53 interaction in coronary restenosis. Science. 265:1994;391-394.
29. Stenberg R.M., Witte P.R., Stinski M.F. Multiple spliced and unspliced transcripts from human cytomegalovirus immediate-early region 2 and evidence for a common initiation site within immediate-early region 1. J. Virol. 56:1985;665-675.
30. Stenberg R.M., Fortney J., Barlow S.W., Magrane B.P., Nelson J.A., Ghazal P. Promoter-specific trans activation and repression by human cytomegalovirus immediate-early proteins involves common and unique protein domains. J. Virol. 64:1990;1556-1565.
31. Wang M.M., Reed R.R. Molecular cloning of the olfactory neuronal transcription factor Olf-1 by genetic selection in yeast. Nature. 364:1993;121-126.
32. Wathen M.W., Stinski M.F. Temporal patterns of human cytomegalovirus transcription: mapping the viral RNAs synthesized at immediate early, early, and late times after infection. J. Virol. 41:1982;462-477.
33. Yoo Y.D., Chiou C.-J., Choi K.S., Yi Y., Michelson S., Kim S., Haywrd G.S., Kim S.-J. The IE2 regulatory protein of human cytomegalovirus induces expression of the human TGF-β1 gene through an Egr-1 binding site. J. Virol. 70:1996;7062-7070.
34. Zhu H., Shen Y., Shenk T. Human cytomegalovirus IE1 and IE2 proteins block apoptosis. J. Virol. 69:1995;7960-7970.