Hexosaminidase-altered aberrant crypts, carrying decreased hexosaminidase α and β subunit mRNAs, in colon of 1,2-dimethylhydrazine-treated rats

Japanese Journal of Cancer Research

Volumn 92, Issue 2, 2001, Pages 109-118

  Tsukamoto, Tetsuya ^a   Fukami, Hiroko ^a   Yamanaka, Shoji ^b   Yamaguchi, Akira ^b   Nakanishi, Hayao ^a   Sakai, Hiroki ^a,c   Aoki, Ichiro ^b   Tatematsu, Masae ^a  

^a AICHI CANCER CENTER HOSPITAL   (Japan)

^b YOKOHAMA CITY UNIVERSITY   (Japan)

^c GIFU UNIVERSITY   (Japan)

Author keywords

Aberrant crypt foci; Crypt isolation; Enzyme (hexosaminidase) altered foci; LightCycler; Real time PCR

Indexed keywords

1,2 DIMETHYLHYDRAZINE; BETA ACTIN; BETA N ACETYLHEXOSAMINIDASE; BETA N ACETYLHEXOSAMINIDASE A; BETA N ACETYLHEXOSAMINIDASE B; BIOLOGICAL MARKER; MESSENGER RNA; PROTEIN SUBUNIT;

ALPHA CHAIN; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BETA CHAIN; CELL ISOLATION; CELL STRUCTURE; COLON CANCER; CONTROLLED STUDY; CRYPT CELL; DOWN REGULATION; DRUG INDUCED DISEASE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME HISTOCHEMISTRY; INTESTINE CRYPT; MALE; MOLECULAR BIOLOGY; NONHUMAN; PRECANCER; PRIORITY JOURNAL; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STAINING; STROMA CELL; STRUCTURE ANALYSIS;

1,2-DIMETHYLHYDRAZINE; ANIMALS; BETA-N-ACETYLHEXOSAMINIDASE; COLON; COLONIC NEOPLASMS; DOWN-REGULATION; HISTOCYTOCHEMISTRY; MALE; RATS; RATS, INBRED F344; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TUMOR MARKERS, BIOLOGICAL;

EID: 0035079936     PISSN: 09105050     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1349-7006.2001.tb01072.x     Document Type: Article

References (51)

1. Colorectal tumors
2. Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: Preliminary findings
3. Aberrant crypts: Potential preneoplastic lesions in the murine colon
4. Sequential analyses of the growth and morphological characteristics of aberrant crypt foci: Putative preneoplastic lesions
5. Dose response and proliferative characteristics of aberrant crypt foci: Putative preneoplastic lesions in rat colon
6. β-Hexosaminidase isozymes in human colonic carcinoma
7. Isoenzymes of β-hexosaminidase from normal rat colon and colonic carcinoma
8. Glycoprotein metabolism in normal proximal and distal rat colon and changes associated with 1,2-dimethylhydrazine-induced colonic neoplasia
9. Colonic aberrant crypts in azoxymethane-treated F344 rats have decreased hexosaminidase activity
10. Enzyme-altered foci in colons of carcinogen-treated rats
11. Two types of putative preneoplastic lesions identified by hexosaminidase activity in whole-mounts of colons from F344 rats treated with carcinogen
12. Aberrant crypt foci and colon tumors in F344 rats have similar increases in proliferative activity
13. Use of azoxymethane-induced foci of aberrant crypts in rat colon to identify potential cancer chemopreventive agents
14. Aberrant crypt foci in the colonic mucosa of rats treated with a genotoxic and nongenotoxic colon carcinogen
15. Chemopreventive agents-induced regression of azoxymethane-induced aberrant crypt foci with the recovery of hexosaminidase activity
16. The GM2 gangliosidosis
17. Structure and expression of the mouse β-hexosaminidase genes, Hexa and Hexb
18. Targeted disruption of the Hexa gene results in mice with biochemical and pathologic features of Tay-Sachs disease
19. Mouse models of Tay-Sachs and Sandhoff diseases differ in neurologic phenotype and ganglioside metabolism
20. Methods for the isolation of intact epithelium from the mouse intestine
21. Development of aberrant crypt foci involves a fission mechanism as revealed by isolation of aberrant crypts
22. Development and distribution of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-induced aberrant crypt foci in the rat large intestine
23. Histochemical demonstration of N-acetyl-β-glucosaminidase employing naphthol AS-BI N-acetyl-β-glucosaminide as substrate
24. A quantitative study of N-acetyl-β-glucosaminidase activity in unfixed tissue sections of the guinea-pig thyroid gland
25. "Fundamentals of Biostatistics"
26. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenolchloroform extraction
27. In situ hybridization with non-radioactive digoxigenin-11-UTP-labeled cRNA probes: Localization of developmentally regulated mouse tenascin mRNAs
28. A novel methodological approach to study the level of specific protein and gene expression in aberrant crypt foci putative preneoplastic colonic lesions by Western blotting and RT-PCR
29. Molecular analysis of the protein tyrosine phosphatase gamma gene in human lung cancer cell lines
30. Isolation of oncogenes from rat mammary tumors by a highly efficient retrovirus expression cloning system
31. The nucleotide sequence of the rat cytoplasmic β-actin gene
32. Quantitative RT-PCR: Pitfalls and potential
33. Role of aberrant crypt foci in understanding the pathogenesis of colon cancer
34. Alkaline phosphatase activity and basophilia in hepatic cells following administration of butter yellow to rats
35. Histochemistry of gamma-glutamyl transpeptidase in rat liver during aflatoxin B1-induced carcinogenesis
36. Effects of a choline-devoid diet on the emergence of gamma-glutamyltranspeptidase-positive foci in the liver of carcinogen-treated rats
37. Multistage hepatocarcinogenesis
38. Effects of a high-sucrose diet on the development of enzyme-altered foci in chemical hepatocarcinogenesis in rats
39. Inhibition by pentachlorophenol of the initiating and promoting activities of 1′-hydroxysafrole for the formation of enzyme-altered foci and tumors in rat liver
40. Examination of enzyme-altered foci with gamma-glutamyl transpeptidase, aldehyde dehydrogenase, glucose-6-phosphate dehydrogenase, and other markers in methacrylate-embedded liver
41. Immunohistochemical demonstration of induction of pyloric glands with low pepsinogen 1 (Pg 1) content in rat stomach by N-methyl-N′-nitro-N-nitrosoguanidine
42. Immunohistochemical demonstration of pyloric gland-type cells with low-pepsinogen isozyme 1 in preneoplastic and neoplastic tissues of rat stomachs treated with N-methyl-N′-nitro-N-nitrosoguanidine
43. Synergism between sodium chloride and sodium taurocholate and development of pepsinogen-altered pyloric glands: Relevance to a medium-term bioassay system for gastric carcinogens and promoters in rats
44. Proliferation kinetics of pepsinogen altered pyloric gland cells in rats treated with N-methyl-N′-nitro-N-nitrosoguanidine
45. Enhancing effects of various gastric carcinogens on development of pepsinogen-altered pyloric glands in rats
46. DNA methylation of the pepsinogen 1 gene during rat glandular stomach carcinogenesis induced by N-methyl-N′-nitro-N-nitrosoguanidine or catechol
47. Dose-dependent induction of both pepsinogen-altered pyloric glands and adenocarcinomas in the glandular stomach of C3H mice treated with N-methyl-N-nitrosourea
48. The LightCycler: A micro-volume multisample fluorimeter with rapid temperature control
49. Continuous fluorescence monitoring of rapid cycle DNA amplification
50. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification
51. Real time quantitative PCR