Optimization of transformation procedures in avermectin high-producing streptomyces avermitilis

Biotechnology Letters

Volumn 23, Issue 6, 2001, Pages 457-462

  Hwang, Yong Soon ^a,b   Lee, Jae Young ^a,b   Kim, Eung Soo ^c   Choi, Cha Yong ^a,b  

^a Seoul National University   (South Korea)

^b Korea Biotechnology Center   (South Korea)

^c Hankuk University of Foreign Studies   (South Korea)

Author keywords

Avermectin; Electroporation; Protoplast; Streptomyces avermitilis; Transformation

Indexed keywords

AVERMECTIN;

ARTICLE; BACTERIUM MUTANT; BACTERIUM TRANSFORMATION; ELECTROPORATION; NONHUMAN; PH; PRODUCTIVITY; PROTOPLAST; STREPTOMYCES; TECHNIQUE;

BACTERIA (MICROORGANISMS); STREPTOMYCES; STREPTOMYCES AVERMITILIS;

EID: 0035065152     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1010377219368     Document Type: Article

References (14)

1. Screening of spontaneous and induced mutants in Streptornyces avermitilis enhances avermectin production
2. Transformation of plasmid DNA into Streptomyces at high frequency
3. Avermectins, new family of potent anthelmintic agents: Producing organisms and fermentation
4. Plasmid transformation of Streptomyces tendae after heat attenuation of restriction
5. Genetic relationships among actinomycetes that produce the immuno-suppressant macrolides FK506, FK520/FK523 and rapamycin
6. Organization of the biosynthetic gene cluster for the polyketide anthelmintic macrolide avermectin in Streptomyces avermitilis
7. Effect of a global regulatory gene, afsR2, from Streptomyces lividans on avermectin production in Streptomyces avermitilis
8. Transformation of Streptomyces avermitilis by plasmid DNA
9. Electroporation of intact cells of Streptomyces parvulus and Streptomyces vinaceus
10. A simple and rapid method of transformation of Streptomyces rimosus R6 and other streptomycetes by electroporation
11. Cloning and sequence analysis of the putative rifamycin polyketide synthase gene cluster from Amycolatopsis mediterranei
12. Genetic instability of the Streptomyces chromosome