Cloning and sequence analysis of the putative rifamycin polyketide synthase gene cluster from Amycolatopsis mediterranei

FEMS Microbiology Letters

Volumn 159, Issue 2, 1998, Pages 201-207

  Schupp, Thomas ^a   Toupet, Christiane ^a   Engel, Nathalie ^a   Goff, Stephen ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

Amycolatopsis mediterranei; Ansamycin; Cloning; Polyketide synthase; Polyketide synthase gene cluster; Rifamycin; Sequence analysis

Indexed keywords

ANSAMYCIN DERIVATIVE; BACTERIAL ENZYME; HYDROLYASE; LIGASE; OXIDOREDUCTASE; POLYKETIDE; POLYKETIDE SYNTHASE; PROPIONIC ACID; RIFAMYCIN; SYNTHETASE; TRANSFERASE; UNCLASSIFIED DRUG;

ACTINOBACTERIA; ANTIBIOTIC BIOSYNTHESIS; ARTICLE; BACTERIAL GENETICS; CONTROLLED STUDY; DNA SEQUENCE; ESCHERICHIA COLI; GENE CLUSTER; MOLECULAR CLONING; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN DOMAIN; SEQUENCE ANALYSIS; SOIL MICROFLORA;

ACTINOMYCETALES; CLONING, MOLECULAR; GENES, BACTERIAL; MULTIENZYME COMPLEXES; MULTIGENE FAMILY; OPEN READING FRAMES; RIFAMYCINS;

ACTINOBACTERIA (CLASS); AMYCOLATOPSIS MEDITERRANEI; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; UNCULTURED ACTINOMYCETE; UNIDENTIFIED BACTERIOPHAGE;

EID: 0032519914     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00569-7     Document Type: Article

References (20)

1. Wehrli, W. (1977) Ansamycins. Chemistry, Biosynthesis and Biological Activity. Topics Curr. Chem. 72, 21-49.
2. Brogden, R.N. and Fitton, A. (1994) Rifabutin. A review of its antimicrobial activity, pharmacokinetic properties and therapeutic efficacy. Drugs 47, 983-1009.
3. Oppenheim, B., Koornhof, H.J. and Austrian, R. (1986) Antibiotic-resistant pneumococcal disease in children at Baragwanath Hospital, Johannesburg. Pediatr. Infect. Dis. 5, 520-524.
4. Szabo, C., Bissel, M.J. and Calvin, M. (1976) Inhibition of infectious rous sarcoma virus production by a rifamycin derivative. J. Virol. 18, 445-453.
5. Ghisalba, O., Auden, A.L., Schupp, T. and Nüesch, J. (1984) The Rifamycins: Properties, Biosynthesis, and Fermentation. In: Biotechnology and Industrial Antibiotics (Vandamme, E.J., Ed.) pp. 281-327, Decker Inc., New York.
6. Schupp, T. and Divers, M. (1986) Protoplast preparation and regeneration in Nocardia mediterranei. FEMS Microbiol. Lett. 36, 159-162.
7. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
8. Pospiech, A. and Neumann, B. (1995) A versatile quick-prep of genomic DNA from Gram-positive bacteria. Trends Genet. 11, 217.
9. Hopwood, D.A., Bibb, M.J., Chater, K.F., Kieser, T., Bruton, C., Kieser, H., Lydiate, D.J., Smith, C.P., Ward, J.M. and Schrempf, H. (1985) Genetic manipulation of Streptomyces. A laboratory manual. John Innes Foundation, Norwich, UK.
10. Kieser, T. and Melton, R.E. (1988) Plasmid pIJ699, a multi-copy positive-selection vector for Streptomyces. Gene 65, 83-91.
11. Schupp, T., Toupet, C., Cluzel, B., Neff, S., Hill, S., Beck, J. and Ligon, J. (1995) A Sorangium cellulosum (Myxobacterium) gene cluster for the biosynthesis of the macrolide antibiotic soraphen A: cloning, characterization, and homology to polyketide synthase genes from Actinomycetes. J. Bacteriol., 3673-3679.
12. Birnboim, H.C. (1983) A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol. 100, 243-255.
13. Genetics Computer Group (1996) Sequencing analysis sofware package, version 9. University of Wisconsin Genetics Computer Group, Madison.
14. Donadio, S., Staver, M.J., McAlpine, J.B., Swanson, S.J. and Katz, L. (1991) Modular organization of the genes required for complex polyketide biosynthesis. Science 252, 675-679.
15. Donadio, S. and Katz, L. (1992) Organization of the enzymatic domains in the multifunctional polyketide synthase involved in erythromycin formation in Saccharopolyspora erythraea. Gene 111, 51-60.
16. Aparicio, J.F., Molnar, I., Schwecke, T., Konig, A., Haydock, S.F., Khaw, L.E., Staunton, J. and Leadlay, P.F. (1996) Organization of the biosynthetic gene cluster for rapamycin in Streptomyces hygroscopicus: analysis of the enzymatic domains in the modular polyketide synthase. Gene 169, 9-16.
17. Haydock, S.F., Aparicio, J., Molnar, I., Schwecke, T., Khaw, L.E., Konig, A., Marsden, A.F., Galloway, I.S., Staunton, J. and Leadlay, P.F. (1995) Divergent sequence motifs correlated with the substrate specificity of (methyl)malonyl-CoA:acyl carrier protein transacylase domains in modular polyketide synthases. FEBS Lett. 374, 246-248.
18. Ghisalba, O., Fuhrer, H., Richter, W.J. and Moss, S. (1981) A genetic approach to the biosynthesis of the rifamycin-chromophore in Nocardia mediterranei. III. Isolation and identification of an early aromatic ansamycin-precursor containing the seven-carbon amino starter-unit and three initial acetate/propionate-units of the ansa chain. J. Antibiot. 34, 58-63.
19. Ghisalba, O., Traxler, P. and Nüesch, J. (1978) Early intermediates in the biosynthesis of ansamycins. I. Isolation and identification of protorifamycin I. J. Antibiot. 31, 1124-1131.
20. Schupp, T. and Nüesch, J. (1979) Chromosomal mutations in the final step of rifamycin B biosynthesis. FEMS Microbiol. Lett. 6, 23-27.