Human telomerase reverse transcriptase-immortalized MRC-5 and HCA2 human fibroblasts are fully permissive for human cytomegalovirus

Journal of General Virology

Volumn 82, Issue 4, 2001, Pages 855-863

  McSharry, B P ^a   Jones, C J ^a   Skinner, J W ^a   Kipling, D ^a   Wilkinson, G W G ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; BETA GALACTOSIDASE; CD34 ANTIGEN; COMPLEMENTARY DNA; DNA POLYMERASE; GUANINE; PROTIRELIN DERIVATIVE; RAP PROTEIN; RESTRICTION ENDONUCLEASE; RNA DIRECTED DNA POLYMERASE; TANKYRASE; TELOMERASE; THYMINE; VIRUS DNA; VIRUS ENZYME; VIRUS RNA; VIRUS VACCINE; VIRUS VECTOR;

ARTICLE; BIOTECHNOLOGY; CELL CLONING; CELL GROWTH; CELL IMMORTALIZATION; CELL LINE; CELL POPULATION; CELL STRUCTURE; CONTROLLED STUDY; CYTOLOGY; CYTOMEGALOVIRUS INFECTION; DELETION MUTANT; DIPLOIDY; ENZYME ACTIVITY; ENZYME ANALYSIS; EPISOME; EPSTEIN BARR VIRUS; FIBROBLAST CULTURE; GENE EXPRESSION; GENE TARGETING; HELPER CELL; HUMAN; HUMAN CELL; HUMAN CYTOMEGALOVIRUS; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE; RETROVIRUS; SENESCENCE; TRANSGENE; VACCINE PRODUCTION; VIROGENESIS; VIRUS DETECTION; VIRUS PLAQUE; VIRUS REPLICATION;

CYTOMEGALOVIRUS; DNA VIRUSES; HUMAN HERPESVIRUS 4; HUMAN HERPESVIRUS 5; RNA VIRUSES; UNIDENTIFIED RETROVIRUS;

EID: 0035063440     PISSN: 00221317     EISSN: None     Source Type: Journal    
DOI: 10.1099/0022-1317-82-4-855     Document Type: Article

References (41)

1. HIV-1 indicator cell lines
2. Human CD8+ T cell responses to EBV EBNA1: HLA class I presentation of the (Gly-Ala)-containing protein requires exogenous processing
3. Extension of life-span by introduction of telomerase into normal human cells
4. Comparison of MRC-5 and U-373MG astrocytoma cells for detection of cytomegalovirus in shell vial centrifugation cultures
5. Human cytomegalovirus clinical isolates carry at least 19 genes not found in laboratory strains
6. An immortalized human fibroblast cell line is permissive for human cytomegalovirus infection
7. Safe and efficient generation of recombinant retroviruses with amphotropic and ecotropic host ranges
8. How might replicative senescence contribute to human ageing?
9. Growth kinetics of human cytomegalovirus are altered in monocyte-derived macrophages
10. Human cytomegalovirus persistently infects aortic endothelial cells
11. Cytomegalovirus replicates in differentiated but not in undifferentiated human embryonal carcinoma cells
12. Cytomegalovirus infection of human teratocarcinoma cells in culture
13. Characteristics of a human cell line transformed by DNA from human adenovirus type 5
14. Defective growth correlates with reduced accumulation of a viral DNA replication protein after low-multiplicity infection by a human cytomegalovirus ie1 mutant
15. Practical protocol for cytomegalovirus isolation: Use of MRC-5 cell monolayers incubated for 2 weeks
16. Mammalian telomeres end in a large duplex loop
17. Human trophoblast cells are permissive to the complete replicative cycle of human cytomegalovirus
18. Association of human cytomegalovirus (HCMV) with mink and rabbit lung cells
19. Characteristics of a human diploid cell designated MRC-5
20. Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype
21. Specific association of human telomerase activity with immortal cells and cancer
22. TIN2, a new regulator of telomere length in human cells
23. In-vitro cultivation of human cytomegalovirus in thyroid epithelial cells
24. Human cytomegalovirus inhibits human immunodeficiency virus replication in cells productively infected by both viruses
25. Up to 100-fold increase of apparent gene expression in the presence of Epstein-Barr virus oriP sequences and EBNA1: Implications of the nuclear import of plasmids
26. Identification of human Rap1: Implications for telomere evolution
27. Comparison of MRC-5 and continuous cell lines for detection of cytomegalovirus in centrifugation cultures
28. Absence of cancer-associated changes in human fibroblasts immortalized with telomerase
29. Advanced mammalian gene transfer: High titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging cell line
30. Use of recombinant plasmids to investigate the structure of the human cytomegalovirus genome
31. Cell types involved in replication and distribution of human cytomegalovirus
32. Fibroblasts, epithelial cells, endothelial cells and smooth muscle cells are major targets of human cytomegalovirus infection in lung and gastrointestinal tissues
33. Modification of human cytomegalovirus tropism through propagation in vitro is associated with changes in the viral genome
34. Tankyrase, a poly(ADP-ribose) polymerase at human telomeres
35. Reactivation of latent human cytomegalovirus by allogeneic stimulation of blood cells from healthy donors
36. Control of telomere length by the human telomeric protein TRF1
37. TRF2 protects human telomeres from end-to-end fusions
38. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT
39. Telomerase prevents the accelerated cell ageing of Werner syndrome fibroblasts
40. Cell-cycle-regulated association of RAD50/MRE11/NBS1 with TRF2 and human telomeres