Sequence-specific termination by T7 RNA polymerase requires formation of paused conformation prior to the point of RNA release

Genes to Cells

Volumn 6, Issue 4, 2001, Pages 291-301

  Song, H ^a   Kang, C ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

CYTIDINE TRIPHOSPHATE; GUANOSINE TRIPHOSPHATE; NUCLEIC ACID BASE; RNA POLYMERASE; URACIL; URIDINE TRIPHOSPHATE;

ARTICLE; BACTERIOPHAGE T7; BASE PAIRING; COMPLEX FORMATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; ESCHERICHIA COLI; GENETIC CONSERVATION; MUTATION; NONHUMAN; PRIORITY JOURNAL; REGULATOR GENE; RNA CONFORMATION; RNA SEQUENCE; RNA STRUCTURE; RNA TRANSLATION; SIGNAL TRANSDUCTION;

BACTERIOPHAGE T7; BASE SEQUENCE; DNA PRIMERS; DNA, RECOMBINANT; DNA-DIRECTED RNA POLYMERASES; MOLECULAR SEQUENCE DATA; PLASMIDS; PROTEIN CONFORMATION; RNA, BACTERIAL; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 0035050155     PISSN: 13569597     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2443.2001.00420.x     Document Type: Article

References (37)

1. Pausing by bacterial RNA polymerase is mediated by mechanistically distinct classes of signals
2. Multiple interactions stabilize a single paused transcription intermediate in which hairpin to 3′ end spacing distinguishes pause and termination pathway
3. Structural basis for initiation of transcription from an RNA polymerase-promoter complex
4. Structure of a transcribing T7 RNA polymerase initiation complex
5. The 9S RNA precursor of Escherichia coli 5S RNA has three structural domains: Implications for processing
6. Single-molecule study of transcriptional pausing and arrest by E. coli RNA polymerase
7. Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements
8. The mechanism of intrinsic transcription termination
9. Intrinsic termination of T7 RNA polymerase mediated by either RNA or DNA
10. Characterization of an unusual, sequence-specific termination signal for T7 RNA polymerase
11. An integrated model of the transcription complex in elongation, termination, and editing
12. The elongation-termination decision in transcription
13. T7 RNA polymerase elongation complex structure and movement
14. Mechanisms by which T7 lysozyme specifically regulates T7 RNA polymerase during different phases of transcription
15. Interactions of the RNA polymerase of bacteriophage T7 with its promoter during binding and initiation of transcription
16. Transcription termination by bacteriophage T7 RNA polymerase at rho-independent terminators
17. Transcription termination in vitro by bacteriophage T7 RNA polymerase: The role of sequence elements within and surrounding a rho-independent transcription terminator
18. Structure of T7 RNA polymerase complexed to the transcriptional inhibitor T7 lysozyme
19. Transcriptional arrest: Escherichia coli RNA polymerase translocates backward, leaving the 3′ end of the RNA intact and extruded
20. Bipartite modular structure of intrinsic, RNA hairpin-independent termination signal for phage RNA polymerases
21. Mutant bacteriophage T7 RNA polymerase with altered termination properties
22. Pausing and termination by bacteriophage T7 RNA polymerase
23. Characterization of two types of termination signal for bacteriophage T7 RNA polymerase
24. Termination and slippage by bacteriophage T7 RNA polymerase
25. Single-stranded DNA 'blue' T7 promoter plasmids: A versatile tandem promoter system for cloning and protein engineering
26. The RNA-DNA hybrid maintains the register of transcription by preventing backtracking of RNA polymerase
27. Studies on the interaction of T7 RNA polymerase with a DNA template containing a site-specifically placed psoralen cross-link. I. Characterization of elongation complexes
28. Nuclease activity of T7 RNA polymerase and the heterogeneity of transcription elongation complexes
29. Two-site contact of elongating transcripts to phage T7 RNA polymerase at C-terminal regions
30. Crystal structure of bacteriophage T7 RNA polymerase at 3.3 angstrom resolution
31. Model for the mechanism of bacteriophage T7 RNAP transcription and termination
32. Use of T7 RNA polymerase to direct expression of cloned genes
33. Efficient recovery of infectious vesicular stomatitis virus entirely from cDNA clones
34. Mechanism of intrinsic transcription termination and antitermination
35. Nonequilibrium mechanism of transcription termination from observations of single RNA polymerase molecules
36. Isolation of transcriptionally active mutants of T7 RNA polymerase that do not support phage growth