New series of vectors for phage display and prokaryotic expression of proteins

BioTechniques

Volumn 30, Issue 4, 2001, Pages 720-726

  Paschke, M ^a   Zahn, G ^a   Warsinke, A ^a   Hohne W ^a  

^a HUMBOLDT UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GREEN FLUORESCENT PROTEIN;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; DNA VECTOR; PHAGE DISPLAY; POLYMERASE CHAIN REACTION; PROKARYOTE; PROTEIN DEGRADATION; PROTEIN EXPRESSION;

ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENES, REPORTER; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; INDICATORS AND REAGENTS; LUMINESCENT PROTEINS; MUTAGENESIS, INSERTIONAL; PECTOBACTERIUM CAROTOVORUM; PEPTIDE LIBRARY;

PROKARYOTA;

EID: 0035046537     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (17)

1. A surface expression vector for antibody screening
2. Phage display
3. Improved green fluorescent protein by molecular evolution using DNA shuffling
4. A family of vectors for surface display and production of antibodies
5. Phage-display libraries of murine and human antibody Fab fragments
6. Reliable cloning of functional antibody variable domains from hybridomas and spleen cell repertoires employing a reengineered phage display system
7. Characterization of neutralizing anti-pre-S1 and anti-pre-S2 (HBV) monoclonal antibodies and their fragments
8. By-passing immunization. Human antibodies from V-gene libraries displayed on phage
9. Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase
10. The identity of the base following the stop codon determines the efficiency of in vivo translational termination in Escherichia coli
11. Use of the tetracycline promoter for the tightly regulated production of a murine antibody fragment in Escherichia coli
12. Filamentous fusion phage: Novel expression vectors that display cloned antigens on the virion surface
13. Phage display technology in antibody engineering: Design of phagemid vectors and in vitro maturation systems
14. The downstream box: An efficient and independent translation initiation signal in Escherichia coli
15. Site-directed mutagenesis of double-stranded DNA by the polymerase chain reaction
16. Phage on display
17. Investigation of a tetracycline-regulated phage display system