Primer-independent RNA sequencing with bacteriophage φ6 RNA polymerase and chain terminators

RNA

Volumn 7, Issue 5, 2001, Pages 774-781

  Makeyev, E V ^a   Bamford, D H ^a  

^a UNIVERSITY OF HELSINKI   (Finland)

Author keywords

Bacteriophage 6; Bluetongue virus; Chain terminators; DsRNA virus; RNA sequencing; RNA dependent RNA polymerase

Indexed keywords

DNA POLYMERASE; DOUBLE STRANDED RNA; RNA; RNA POLYMERASE;

ARTICLE; BACTERIOPHAGE; BLUETONGUE ORBIVIRUS; CONTROLLED STUDY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RNA SEQUENCE; RNA VIRUS;

BACTERIOPHAGE PHI 6; BASE SEQUENCE; BLUETONGUE VIRUS; CODON, TERMINATOR; DEOXYADENINE NUCLEOTIDES; DEOXYCYTOSINE NUCLEOTIDES; DEOXYGUANINE NUCLEOTIDES; DEOXYURACIL NUCLEOTIDES; MOLECULAR SEQUENCE DATA; RNA; RNA REPLICASE; RNA, DOUBLE-STRANDED; RNA, VIRAL; SEQUENCE ANALYSIS, RNA; TEMPLATES, GENETIC;

BLUETONGUE VIRUS; DSRNA VIRUSES; RNA VIRUSES; UNIDENTIFIED BACTERIOPHAGE;

EID: 0035043182     PISSN: 13558382     EISSN: None     Source Type: Journal    
DOI: 10.1017/S1355838201002060     Document Type: Article

References (29)

1. Methods for subtyping and molecular comparison of human viral genomes
2. Strategies for the sequence determination of viral dsRNA genomes
3. Transcription from bacteriophage T7 and SP6 RNA polymerase promoters in the presence of 3′deoxyribonucleoside 5′-triphosphate chain terminators
4. Qβ replicase template specificity: Different templates require different GTP concentrations for initiation
5. A mechanism for initiating RNA-dependent RNA polymerization
6. Crystallization and preliminary X-ray crystallographic studies on the bacteriophage φ6 RNA-dependent RNA polymerase
7. Isolation and identification of bluetongue virus
8. Mapping adenines, guanines, and pyrimidines in RNA
9. Noncoding RNA genes
10. In vitro packaging and replication of individual genomic segments of bacteriophage φ6 RNA
11. Complete sequence of constant and 3′ noncoding regions of an immunoglobulin mRNA using the dideoxynucleotide method of RNA sequencing
12. RNA motifs that determine specificity between a viral replicase and its promoter
13. A long-range pseudoknot in Qβ RNA is essential for replication
14. Co-translational folding of an eukaryotic multidomain protein in a prokaryotic translation system
15. RNA sequencing with radioactive chain-terminating ribonucleotides
16. Replicase activity of purified recombinant protein P2 of double-stranded RNA bacteriophage φ6
17. The polymerase subunit of a dsRNA virus plays a central role in the regulation of viral RNA metabolism
18. A new method for sequencing DNA
19. Non-isotopic oligonucleotide probes: An effective replacement for isotopic probes in molecular biology techniques
20. Analysis of the terminal sequences of the genome segments of four orbiviruses
21. Minus-strand initiation by brome mosaic virus replicase within the 3′ tRNA-like structure of native and modified RNA templates
22. Marked infidelity of human immunodeficiency virus type 1 reverse transcriptase at RNA and DNA template ends
23. Direct chemical method for sequencing RNA
24. DNA sequencing with chain-terminating inhibitors
25. Transcriptional sequencing: A method for DNA sequencing using RNA polymerase
26. 3′-terminal nucleotide sequence of encephalomyocarditis virus RNA determined by reverse transcriptase and chain-terminating inhibitors