Identification of residues critical for enzymatic activity in the domain encoded by exons 8 and 9 of the human inducible nitric oxide synthase

American Journal of Respiratory Cell and Molecular Biology

Volumn 24, Issue 5, 2001, Pages 616-620

  Eissa, N Tony ^a,b   Haggerty, Cynthia M ^a,b,c   Palmer, Celeste D ^a,b   Patton, Walter ^a,b,d   Moss, Joel ^a,b  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b NATIONAL HEART LUNG AND BLOOD INSTITUTE   (United States)

^c NATIONAL CANCER INSTITUTE   (United States)

^d LEBANON VALLEY COLLEGE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMINO ACID; AMINO ACID SUBSTITUTION; CHEMICAL MUTAGENESIS; DIMERIZATION; ENZYME ACTIVITY; EXON; IMMUNOBLOTTING; MUTANT PROTEIN; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; PATHOGENICITY; PROTEIN PRODUCTION; SDS POLYACRYLAMIDE GEL ELECTROPHORESIS; WILD RELATIVE;

EID: 0035033291     PISSN: 10441549     EISSN: None     Source Type: Journal    
DOI: 10.1165/ajrcmb.24.5.4324     Document Type: Article

References (28)

1. 1. Palmer, R. M., A. G. Ferrige, and S. Moncada. 1987. Nitric oxide release accounts for the biological activity of endothelium-derived relaxing factor. Nature 327:524-526.
2. 2. Guo, F. H., S. A. A. Comhair, S. Zheng, R. A. Dweik, N. T. Eissa, M. J. Thomassen, W. Calhoun, and S. C. Erzurum. 2000. Molecular mechanisms of increased nitric oxide (NO) in asthma: evidence for transcriptional and post-translational regulation of NO synthesis. J. Immunol. 164:5970-5980.
3. 3. Marletta, M. A. 1994. Nitric oxide synthase: aspects concerning structure and catalysis. Cell 78:927-930.
4. 4. Nathan, C., and Q.-W. Xie. 1994. Nitric oxide synthases: roles, tolls, and controls. Cell 78:915-918.
5. 5. Xie, Q.-W., H. J. Cho, J. Calaycay, R. A. Mumford, K. M. Swiderek, T. D. Lee, A. Ding, T. Troso, and C. Nathan. 1992. Cloning and characterization of inducible nitric oxide synthase from mouse macrophages. Science 256: 225-228.
6. 6. Abu-Soud, H. M., and D. J. Stuehr. 1993. Nitric oxide synthases reveal a role for calmodulin in controlling electron transfer. Proc. Natl. Acad. Sci. USA 90:10769-10772.
7. 7. Cho, H. J., Q.-W. Xie, J. Calaycay, R. A. Mumford, K. M. Swiderek, T. D. Lee, and C. Nathan. 1992. Calmodulin is a subunit of nitric oxide synthase from macrophages. J. Exp. Med. 176:599-604.
8. 8. Kobzik, L., D. S. Bredt, C. J. Lowenstein, J. Drazen, B. Gaston, D. Sugarbaker, and J. S. Stamler. 1993. Nitric oxide synthase in human and rat lung: immunocytochemical and histochemical localization. Am. J. Respir. Cell Mol. Biol. 9:371-377.
9. 9. Guo, F. H., H. R. De Raeve, T. W. Rice, D. J. Stuehr, F. B. J. M. Thunissen, and S. C. Erzurum. 1995. Continuous nitric oxide synthesis by inducible nitric oxide synthase in normal human airway epithelium in vivo. Proc. Natl. Acad. Sci. USA 92:7809-7813.
10. 10. Nathan, C. 1997. Inducible nitric oxide synthase: what difference does it make? J. Clin. Invest. 100:2417-2423.
11. 11. Schmidt, H. H. H.W., and U. Walter. 1994. NO at work. Cell 78:919-925.
12. 12. Gunasegaran, K., J.-H. Chen, S. Mahalingam, C. F. Nathan, and J. D. MacMicking. 1998. Rapid Interferon-γ-dependent clearance of influenza A virus and protection from consolidating pneumonitis in nitric oxide synthase 2-deficient mice. J. Exp. Med. 188:1541-1546.
13. 13. Hobbs, A. J., A. Higgs, and S. Moncada. 1999. Inhibition of nitric oxide synthase as a potential therapeutic target. Annu. Rev. Pharamacol. Toxicol. 39:191-220.
14. 14. Stuehr, D. J., and O. W. Griffith. 1992. Mammalian nitric oxide synthases. Adv. Enzymol. Relat. Areas Mol. Biol. 65:287-346.
15. 15. Geller, D. A., C. J. Lowenstein, R. A. Shapiro, A. K. Nussler, M. Di Silvio, S. C. Wang, D. K. Nakayama, R. L. Simmons, S. H. Snyder, and T. R. Billiar. 1993. Molecular cloning and expression of inducible nitric oxide synthase from human hepatocytes. Proc. Natl. Acad. Sci. USA 90:3491-3495.
16. 16. Chartrain, N. A., D. A. Geller, P. P. Koty, N. F. Sitrin, A. K. Nussler, E. P. Hoffman, T. R. Billiar, N. I. Hutchinson, and J. S. Mudgett. 1994. Molecular cloning, structure, and chromosomal localization of the human inducible nitric oxide synthase gene. J. Biol. Chem. 269:6765-6772.
17. 17. Crane, B. R., A. S. Arvai, R. Gachhui, C. Wu, D. K. Ghosh, E. D. Getzoff, D. J. Stuehr, and J. A. Tainer. 1997. The structure of nitric oxide synthase oxygenase domain and inhibitor complexes. Science 278:425-431.
18. 18. Eissa, N. T., A. J. Strauss, C. M. Haggerty, E. K. Choo, S. C. Chu, and J. Moss. 1996. Alternative splicing of human inducible nitric-oxide synthase mRNA; tissue-specific regulation and induction by cytokines. J. Biol. Chem. 271:27184-27187.
19. 19. Eissa, N. T., J. Yuan, C. M. Haggerty, E. K. Choo, and J. Moss. 1998. Cloning and characterization of human inducible nitric oxide synthase splice variants: a domain, encoded by exons 8 and 9, is critical for dimerization. Proc. Natl. Acad. Sci. USA 95:7625-7630.
20. 20. Klatt, P., K. Schmidt, D. Lehner, O. Glatter, H. P. Bächinger, and B. Mayer. 1995. Structural analysis of porcine brain nitric oxide synthase reveals a role for tetrahydrobiopterin and L-arginine in the formation of an SDS-resistant dimer. EMBO J. 14:3687-3695.
21. 21. Xie, Q.-W., M. Leung, M. Fuortes, S. Sassa, and C. Nathan. 1996. Complementation analysis of mutants of nitric oxide synthase reveals that the active site requires two hemes. Proc. Natl. Acad. Sci. USA 93:4891-4896.
22. 15N]nitrate in biological fluids. Anal. Biochem. 126:131-138.
23. 23. Crane, B. R., A. S. Arvai, D. K. Ghosh, C. Wu, E. D. Getzoff, D. J. Stuehr, and J. A. Tainer. 1998. Structure of nitric oxide synthase oxygenase dimer with pterin and substrate. Science 279:2121-2126.
24. 24. Cunningham, B. C., and J. A. Wells. 1989. High-resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science 244:1081-1085.
25. 25. Ghosh, D. K., and D. J. Stuehr. 1995. Macrophage NO synthase: characterization of isolated oxygenase and reductase domains reveals a head-to-head subunit interaction. Biochemistry 34:801-807.
26. 26. Baek, J. K., B. A. Thiel, S. Lucas, and D. J. Stuehr. 1993. Macrophage nitric oxide synthase subunits: purification, characterization, and role of prosthetic groups and substrate in regulating their association into a dimeric enzyme. J. Biol. Chem. 268:21120-21129.
27. 27. Gachhui, R., D. K. Gosh, C. Wu., J. Parkinson, B. R. Crane, and D. J. Stuehr. 1997. Mutagenesis of acidic residues in the oxygenase domain of inducible nitric-oxide synthase identifies a glutamate involved in arginine binding. Biochemistry 36:5097-5103.
28. 28. Cho, H. J., E. Martin, Q.-W. Xie, S. Sassa, and C. Nathan. 1995. Inducible nitric oxide synthase: identification of amino acid residues essential for dimerization and binding of tetrahydrobiopterin. Proc. Natl. Acad. Sci. USA 92:11514-11518.