Assignment of the disulfide bonds of huwentoxin-II by Edman degradation sequencing and stepwise thiol modification

European Journal of Biochemistry

Volumn 268, Issue 8, 2001, Pages 2301-2307

  Shu, Qin ^a   Huang, Renhuai ^b   Liang, Songping ^a,b  

^a PEKING UNIVERSITY   (China)

^b HUNAN NORMAL UNIVERSITY   (China)

Author keywords

Disulfide bond; Edman degradation sequencing; Huwentoxin II; MALDI TOF MS; Thiol modification

Indexed keywords

HUWENTOXIN II; INSECTICIDE; PEPTIDE; SPIDER VENOM; THIOL DERIVATIVE; UNCLASSIFIED DRUG;

ARTICLE; DISULFIDE BOND; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; SPIDER; TOXIN ANALYSIS;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CYSTEINE; DISULFIDES; KINETICS; MOLECULAR SEQUENCE DATA; PHENYLTHIOHYDANTOIN; PYRIDINES; SEQUENCE ANALYSIS, PROTEIN; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPIDER VENOMS; SULFHYDRYL COMPOUNDS; TRYPSIN;

ARANEAE; ORNITHOCTONUS HUWENA;

EID: 0035022764     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.2001.02109.x     Document Type: Article

References (16)

1. Purification and characterization of huwentoxin-II, a neurotoxic peptide from the venom of the Chinese bird spider Selenocosmia huwena
2. Tarantula (Eurypelma californicum) venom, a multicomponent system
3. Primary structures of two proteins from the venom of the Mexican red knee tarantula (Brachypelma smithii)
4. A common structural motif incorporating a cystine knot and a triple-stranded b-sheet in toxic and inhibitory polypeptides
5. Three dimensional structure analysis of μ-agatoxins: Further evidence for common motifs among neurotoxins with diverse ion channel specificities
6. The cystine knot structure of ion channel toxins and related polypeptides
7. Disulfide structure of highly bridged peptides: A new strategy for analysis
8. Echistatin disulfide bridges: Selective reduction and linkage assignment
9. A novel methodology for assignment disulfide bond pairings in proteins
10. Determination of disulfide structure in Agouti-related protein (AGRP) by stepwise reduction and alkaylation
11. Sequence analysis of cysteine and cystine containing peptides and proteins by a gas-phase sequencer with isocratic separation of PTH-amino acids
12. Assignment of the three disulfide bridges of Huwentoxin-I, a neurotoxin from the spider Seleno-cosmia huwena
13. Proton nuclear magnetic resonance studies on huwentoxin-I from the venom of the spider Selenocosmia huwena: 2. Three-dimensional structure in solution
14. Assignment of the three disulfide bonds of Selenocosmia huwena lectin-I from the venom of spider Selenocosmia huwena
15. Three-dimentional structure of Selenocosmia huwena lectin-I from the venom of the spider Selenocosmia huwena
16. Min-21 and Min-23, the smallest peptides that fold like a cystine-stabilized β-sheet motif: Design, solution structure, and thermal stability