The rescue by phage display of human Fabs to gp120 HIV-1 glycoprotein using EBV transformed lymphocytes

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 17, Issue 2, 2001, Pages 97-108

  Kempf, Emmanuelle ^a   Weiss, Etienne ^a   Klein, Philippe ^b   Glacet, Arnaud ^b   Spratt, Spencer ^a   Bourel, Dominique ^b   Orfanoudakis, Georges ^a  

^a UNIVERSITÉ DE STRASBOURG   (France)

^b Etablissement de Lille   (France)

Author keywords

EBV infection; Fab phage culture; HIV 1 gp120; Human recombinant Fabs; Phage display

Indexed keywords

GLYCOPROTEIN GP 120; IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN HEAVY CHAIN; IMMUNOGLOBULIN LIGHT CHAIN; RECOMBINANT ANTIBODY;

ANTIBODY PRODUCTION; ARTICLE; B LYMPHOCYTE; CELL IMMORTALIZATION; EPSTEIN BARR VIRUS; ESCHERICHIA COLI; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; HYBRIDOMA; LYMPHOCYTE TRANSFORMATION; PHAGE DISPLAY;

ESCHERICHIA COLI; HUMAN HERPESVIRUS 4; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

EID: 0035008215     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/mb:17:2:097     Document Type: Article

References (33)

1. 1. Olsson, L., Kronstrom, H., Cambon-De Mouzon, A., Honsik, C., Brodin, T., and Jakobsen, B. (1983) Antibody producing human-human hybridomas, I. Technical aspects. J. Immunol. Methods 61, 17-32.
2. 2. Clackson, T., Hoogenboom, H. R., Griffiths, A. D., and Winter, G. (1991) Making antibody fragments using phage display libraries. Nature 352, 624-628.
3. 3. Burton, D. R., Barbas, C. F., Persson, M. A., Koenig, S., Chanock, R. M., and Lerner, R. A. (1991) A large array of human monoclonal antibodies to type 1 human immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals. Proc. Natl. Acad. Sci. USA 88, 10,134-10,137.
4. 4. Burton, D. R. (1991) Human and mouse monoclonal antibodies by repertoire cloning. Trends Biotechnol. 9, 169-175.
5. 5. Marks, J. D., Hoogenboom, H. R., Bonnert, T. P., McCafferty, J., Griffiths, A. D., and Winter, G. (1991) By-passing immunization. Human antibodies from V-gene libraries displayed on phage. J. Mol. Biol. 222, 581-597.
6. 6. Sheets, M. D., Amersdorfer, P., Finnern, R., et al. (1998) Efficient construction of a large nonimmune phage antibody library: the production of high-affinity human single-chain antibodies to protein antigens. Proc. Natl. Acad. Sci. USA 95, 6157-6162.
7. 7. Barbas, C. F. and Wagner, J. (1995) Synthetic human antibodies: selecting and evolving functional proteins, Methods 8, 94-103.
8. 8. Orfanoudakis, G., Karim, B., Bourel, D., and Weiss, E. (1993) Bacterially expressed Fabs of monoclonal antibodies neutralizing tumour necrosis factor alpha in vitro retain full binding and biological activity. Mol. Immunol. 30, 1519-1528.
9. 9. Barbas, C. F. and Lerner, R. A. (1991) Combinatorial immunoglobulin libraries on the surface of phage (phabs) : rapid selection of antigen-specific Fabs. Methods 2, 119-124.
10. 10. Barbas, C. F., Kang, A. S., Lerner, R. A., and Benkovic, S. J. (1991) Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. Proc. Natl. Acad. Sci. USA 88, 7978-7982.
11. 11. Lefranc, M. P., Giudicelli, V., Busin, C., et al. (1998) IMGT, the International ImMunoGeneTics database. Nucleic Acids Res. 26, 297-303.
12. 12. Kabat, E. A., Wu, T. T., Perry, H. M., Gottesman, K. S., and Foeller, C. (1991) Sequences of proteins of immunological interest. 5th ed. Department of Health and Human Services, Bethesda, MD.
13. 13. Persson, M. A., Caothien, R. H., and Burton, D. R. (1991) Generation of diverse high-affinity human monoclonal antibodies by repertoire cloning. Proc. Natl. Acad. Sci. USA 88, 2432-2436.
14. 14. Sastry, L., Alting-Mees, M., Huse, W.D., et al. (1989) Cloning of the immunological repertoire in Escherichia coli for generation of monoclonal catalytic antibodies: construction of a heavy chain variable region-specific cDNA library. Proc. Natl. Acad. Sci. USA 86, 5728-5732.
15. 15. Cook, G. P. and Tomlinson, I. M. (1995) The human imrnunoglobulin VH repertoire. Immunol. Today 16, 237-242.
16. 16. Frippiat, J. P., Williams, S. C., Tomlinson, I. M., et al. (1995) Organization of the human immunoglobulin lambda light-chain locus on chromosome 22q11.2. Hum. Mol. Genet. 4, 983-991.
17. 17. Victor, K., Andris, J., Pascual, V., and Capra, J. D. (1991) Some cautionary notes on interpreting immunoglobulin variable gene segment restriction in autoimmune disease. Immunol. Ser. 54, 1-12.
18. 18. Duenas, M. and Borrebaeck, C. A. (1994) Clonal selection and amplification of phage displayed antibodies by linking antigen recognition and phage replication. Biotechnology (N. Y.) 12, 999-1002.
19. 19. Coia, G., Ayres, A., Lilley, G. G., and Hudson, P. J., Irving, R.A. (1997) Use of mutator cells as a means for increasing production levels of a recombinant antibody directed against Hepatitis B. Gene 201, 203-209.
20. 20. Ohlin, M. and Borrebaeck, C. A. (1996) Characteristics of human antibody repertoires following active immune responses in vivo. Mol. Immunol. 33, 583-592.
21. 21. Kretzschmar, T. and Geiser, M. (1995) Evaluation of antibodies fused to minor coat protein III and major coat protein VIII of bacteriophage M13. Gene 155, 61-65.
22. 22. Hawkins, R. E. and Winter, G. (1992) Cell selection strategies for making antibodies from variable gene libraries: trapping the memory pool. Eur. J. Immunol. 22, 867-870.
23. 23. Yip, Y. L., Hawkins, N. J., Clark, M. A., and Ward, R. L. (1997) Evaluation of different lymphoid tissue sources for the construction of human immunoglobulin gene libraries. Immunotechnology 3, 195-203.
24. 24. Davenport, C., Bottazzo, G. F., and Todd, I. (1992) Stimulation of human B cells specific for Candida albicans for monoclonal antibody production. FEMS Microbiol. Immunol. 4, 335-343.
25. 25. Klinman, N. R. and Press, J. L. (1975) The B cell specificity repertoire: its relationship to definable subpopulations. Transplant. Rev. 24, 41-83.
26. 26. Rapoport, B., Portolano, S., and McLachlan, S. M. (1995) Combinatorial libraries: new insights into human organ-specific autoantibodies. Immunol. Today 16, 43-49.
27. 27. Siegel, D. L. and Silberstein, L. E. (1994) Expression and characterization of recombinant anti-Rh(D) antibodies on filamentous phage: a model system for isolating human red blood cell antibodies by repertoire cloning. Blood 83, 2334-2344.
28. 28. Wisnewski, A., Cavacini, L., and Posner, M. (1996) Human antibody variable region gene usage in HIV-1 infection. J. Acquir Immune Defic. Syndr. Hum. Retrovirol. 11, 31-38.
29. 29. Collet, T. A., Roben, P., O'Kennedy, R., Barbas, C. F., Burton, D. R., and Lerner, R. A. (1992) A binary plasmid system for shuffling combinatorial antibody libraries. Proc. Natl. Acad. Sci. USA 89, 10,026-10,030.
30. 30. Barbas, C. F., Collet, T. A., Amberg, W., et al. (1993) Molecular profile of an antibody response to HIV-1 as probed by combinatorial libraries. J. Mol. Biol. 230, 812-823.
31. 31. Layne, S. P., Merges, M. J., Dembo, M., Spouge, J. L., and Nara, P. L. (1990) HIV requires multiple gp120 molecules for CD4-mediated infection. Nature 346, 277-279.
32. 32. Zebedee, S. L., Barbas, C. F., Hom, Y. L., et al. (1992) Human combinatorial antibody libraries to hepatitis B surface antigen. Proc. Natl. Acad. Sci. USA 89, 3175-3179.
33. 33. Barbas, C. F., Crowe, J. E., Cababa, D., et al. (1992) Human monoclonal Fab fragments derived from a combinatorial library bind to respiratory syncytial virus F glycoprotein and neutralize infectivity. Proc. Natl. Acad. Sci. USA 89, 10,164-10,168.