Quantification of hepatitis C virus RNA by RT-PCR in comparison to the branched DNA method

Zeitschrift fur Gastroenterologie

Volumn 36, Issue 1, 1998, Pages 5-11

  Roth, W K ^a,c   Lee, J H ^b   Ruster B ^a   Zeuzem, S ^b  

^a INSTITUTE FOR TUMOR BIOLOGY AND EXPERIMENTAL THERAPY   (Germany)

^b Medizinische Klinik III   (Germany)

^c Inst F Transfusionsmedizin I   (Germany)

Author keywords

bDNA; Genotype; HCV; Monitoring; Quantification; RT PCR

Indexed keywords

DNA; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; GENOTYPE; HEPATITIS C; HEPATITIS C VIRUS; HUMAN; INTERMETHOD COMPARISON; MAJOR CLINICAL STUDY; MONITORING; QUANTITATIVE ASSAY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIREMIA;

DNA, VIRAL; GENOTYPE; HEPACIVIRUS; HEPATITIS C; HUMANS; POLYMERASE CHAIN REACTION; RANDOM AMPLIFIED POLYMORPHIC DNA TECHNIQUE; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIREMIA;

EID: 0031911507     PISSN: 00442771     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (23)

1. Choo QL, Kuo G, Weiner AJ et al. Isolation of a cDNA clone derived from a blood-borne non-A, non-B viral hepatitis genome. Science 1989; 244: 359-62
2. Choo QL, Richman KH, Han JH et al. Genetic organization and diversity of the hepatitis C virus. Proc Natl Acad Sci USA 1991; 88: 2451-5
3. Saito I, Miyamura T, Ohbayashi A et al. Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proc Natl Acad Sci USA 1990; 87: 6547-9
4. Davis GL, Balart L, Schiff ER et al. Treatment of chronic hepatitis C with recombinant interferon alfa. A multicenter randomized controlled trial. N Engl J Med 1989; 321: 1501-6
5. Di Bisceglie AM, Martin P, Kassianides C et al. Recombinant interferon alfa therapy for chronic hepatitis C. N Engl J Med 1989; 321: 1506-10
6. Besnard NC, Andre PM. Automated quantitative determination of hepatitis C virus viremia by reverse transcription-PCR. J Clin Microbiol 1994: 32: 1887-93
7. Collins ML, Zayati C, Detmer JJ et al. Preparation and characterization of RNA standards for use in quantitative branched DNA hybridization assays. Anal Biochem 1995; 226: 20-129
8. Hagiwara H, Hayashi N, Mita E et al. Quantitative analysis of hepatitis C virus RNA in serum during interferon alfa therapy. Gastroenterology 1993; 104: 877-83
9. Hino K, Sainokami S, Shimoda K et al. Genotypes and titers of hepatitis C virus for predicting response to interferon in patients with chronic hepatitis C. J Med Virol 1994; 42: 299-305
10. Kobayashi Y, Watanabe S, Konishi M et al. Quantification and typing of serum hepatitis C virus RNA in patients with chronic hepatitis C treated with interferon-β. Hepatology 1993; 18: 1319-25
11. Lau JYN, Davis GL, Kniffen J et al. Significance of serum hepatitis C virus RNA levels in chronic hepatitis C. Lancet 1993; 341: 1501-4
12. Rüster B, Zeuzem S, Roth WK. Quantification of hepatitis C virus RNA by competitive reverse transcription and polymerase chain reaction using a modified hepatitis C virus RNA transcript. Anal Biochem 1995; 224: 597-600
13. Wang AM, Doyle MV, Mark DF. Quantification of mRNA by polymerase chain reaction. Proc Natl Acad Sci USA 1989; 86: 9717-21
14. Sanchez-Pescador R, Sheridan PJ, Detmer JJ et al. Quantitative detection of HCV RNA in human sera by a chemiluminescence signal amplification oligonucleotide probe assay. Hepatology 1992; 16: 588 (abstract 298)
15. Urdea MS, Horn T, Fultz TJ et al. Branched DNA amplification multimers for the sensitive, direct detection of human hepatitis viruses. Nucleic Acids Res Symp Ser 1991; 197-200
16. Zeuzem S, Rüster B, Roth WK. Clinical evaluation of a new polymerase chain reaction assay (Amplicor™ HCV) for detection of hepatitis C virus. Z Gastroenterol 1994; 32: 342-7
17. Chomezynski PN, Sacchi N. Single step method of RNA isolation by acid guanidinium thiocyanatephenol-chloroform extraction. Anal Biochem 1987; 162:156-9
18. Zeuzem S, Rüster B, Lee JH, Stripf T, Roth WK. Evaluation of a reverse hybridization assay for genotyping of hepatitis C virus. J Hepatol 1996; 23: 654-61
19. Van de Peer Y, DeWachter R. Treecon for Windows: A software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Applic Biosc 1994; 10: 569-70
20. Bland JM. Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet I 1986; 307-10
21. Hawkins A, Davidson F, Simmonds P. Comparison of plasma virus loads among individuals infected with hepatitis C virus (HCV) genotypes 1. 2 and 3 by Quantiplex HCV RNA assay versions 1 and 2. Roche Monitor assay, and an in-house limiting dilution method. J Clin Microbiol 1997; 35: 187-92
22. Roth WK, Lee JH. Rüster B, Zeuzem S. Comparison of two quantitative hepatitis C virus reverse transcriptase PCR assays. J Clin Microbiol 1996; 34: 261-4
23. Jacob S, Baudy D, Jones E et al. Comparison of quantitative HCV RNA assays in chronic hepatitis C. Am J Clin Pathol 1997; 107: 362-7