Subcloning, expression, purification, and characterization of Haemophilus influenzae glycerol kinase

Protein Expression and Purification

Volumn 22, Issue 1, 2001, Pages 52-59

  Pawlyk, Aaron C ^a   Pettigrew, Donald W ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLOSTERISM; AMINO ACID SEQUENCE; BACTERIAL DNA; BACTERIAL ENZYME; BINDING SITE; CHEMICAL MUTAGENESIS; DNA LIBRARY; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME SPECIFICITY; FRUCTOSE BISPHOSPHATASE; GLYCEROL KINASE; SEQUENCE HOMOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; HAEMOPHILUS INFLUENZAE;

EID: 0034976830     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2001.1408     Document Type: Article

References (33)

1. An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin, and hsp70 heat shock proteins
2. The actin fold
3. Glycerol dissimilation and its regulation in bacteria
4. Glycerol kinase, the pacemaker for the dissimilation of glycerol in Escherichia coli
5. Feedback inhibition of glycerol kinase, a catabolic enzyme in Escherichia coli
6. Interaction between IIIGlc of the phosphoenolpyruvate: Sugar phosphotransferase system and glycerol kinase of Salmonella typhimurium
7. Allosteric regulation of glycerol kinase by enzyme IIIglc of the phosphotransferase system in Escherichia coli and Salmonella typhimurium
8. Catalytic and allosteric properties of glycerol kinase from Escherichia coli
9. Crystal structures of Escherichia coli glycerol kinase variant S58→W in complex with nonhydrolyzable ATP analogues reveal a putative active conformation of the enzyme as a result of domain motion
10. Glycerol kinase from Escherichia coli and an Ala65→Thr mutant: The crystal structures reveal conformational changes with implications for allosteric regulation
11. Structure of the regulatory complex of Escherichia coli IIIGlc with glycerol kinase
12. Crystal structure of a complex of Escherichia coli glycerol kinase and an allosteric effector fructose 1,6-bisphosphate
13. Synthesis of phosphatides in isolated mitochondria
14. Glycerokinase: Its isolation and properties
15. Crystalline glycerokinase from Candida mycoderma
16. Purification and characterization of glycerol kinase from Trypanosoma brucei
17. Thermostable glycerol kinase from a hyperthermophilic archaeon: Gene cloning and characterization of the recombinant enzyme
18. A novel glycerol kinase from Flavobacterium meningosepticum: Characterization, gene cloning and primary structure
19. Cloning and sequencing of two enterococcal glpK genes and regulation of the encoded glycerol kinases by phosphoenolpyruvate-dependent, phosphotransferase system-catalyzed phosphorylation of a single histidyl residue
20. Cloning, sequencing, high expression, and crystallization of the thermophile Thermus aquaticus glycerol kinase
21. Thermostable glycerol kinase from Thermus flavus: Cloning, sequencing, and expression of the enzyme gene
22. The application of triazine dye affinity chromatography to the large-scale purification of glycerokinase from Bacillus stearothermophilus
23. Catabolite repression and inducer control in Gram-positive bacteria
24. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd
25. Escherichia coli glycerol kinase: Role of a tetramer interface in regulation by fructose 1,6-bisphosphate and phospho-transferase system regulatory protein IIIglc
26. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
27. Inactivation of Escherichia coli glycerol kinase by 5,5′-dithiobis(2-nitrobenzoic acid) and N-ethylmaleimide: Evidence for nucleotide regulatory binding sites
28. Conserved active site aspartates and domain-domain interactions in regulatory properties of the sugar kinase superfamily
29. Calculation of protein extinction coefficients from amino acid sequence data
30. An improved function for fitting sedimentation velocity data for low-molecular-weight solutes
31. Escherichia coli glycerol kinase: Cloning and sequencing of the glpK gene and the primary structure of the enzyme
32. Composition and subunit structure of glycerol kinase from Escherichia coli
33. Amino acid substitution matrices from protein blocks