Review: Protein function at thermal extremes: Balancing stability and flexibility

Comparative Biochemistry and Physiology - A Molecular and Integrative Physiology

Volumn 129, Issue 2-3, 2001, Pages 417-431

  Fields, Peter A ^a  

^a STANFORD UNIVERSITY   (United States)

Author keywords

Compatible solutes; Conformational microstates; Extremophile; Flexibility; Habitat temperature; Protein stability; Psychrophile; Thermal adaptation; Thermophile

Indexed keywords

AMINO ACID; PROTEIN;

AMINO ACID SUBSTITUTION; CATALYSIS; ENVIRONMENTAL TEMPERATURE; ENZYME POLYMORPHISM; LIGAND BINDING; METABOLIC RATE; NONHUMAN; PROTEIN DENATURATION; PROTEIN INTERACTION; PROTEIN MODIFICATION; PROTEIN MOTIF; PROTEIN STABILITY; REVIEW; SEQUENCE HOMOLOGY; TEMPERATURE ACCLIMATIZATION; TEMPERATURE SENSITIVITY; THERMOSTABILITY;

EID: 0034973280     PISSN: 10956433     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1095-6433(00)00359-7     Document Type: Review

References (50)

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2. Lys(13) plays a crucial role in the functional adaptation of the thermophilic triose-phosphate isomerase from Bacillus stearothermophilus to high temperatures
3. Lactate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima: The crystal structure at 2.1-angstrom resolution reveals strategies for intrinsic protein stabilization
4. Protein-folding intermediates: Native-state hydrogen-exchange
5. Enhanced thermal stability of Clostridium beijerinckii alcohol dehydrogenase after strategic substitution of amino acid residues with prolines from the homologous thermophilic Thermoanaerobacter brockii alcohol dehydrogenase
6. Elucidation of determinants of protein stability through genome sequence analysis
7. Occurrence and role of di-myo-inositol-1,1′-phosphate in Methanococcus igneus
8. The effect of metal-ions on the activity and thermostability of the extracellular proteinase from a thermophilic bacillus, strain EA-1
9. Species dependence of fish myosin stability to heat and frozen storage
10. Design and synthesis of new enzymes based on the lactate-dehydrogenase framework
11. Amino acid substitutions in the subunit interface enhancing thermostability of Thermoplasma acidophilum citrate synthase
12. Heat-shock proteins, molecular chaperones, and the stress response: Evolutionary and ecological physiology
13. Molecular adaptations of enzymes from psychrophilic organisms
14. Psychrophilic enzymes: Molecular basis of cold adaptation
15. Hot spots in cold adaptation: Localized increases in conformational flexibility in lactate dehydrogenase A4 orthologs of Antarctic notothenioid fishes
16. An additional aromatic interaction improves the thermostability and thermophilicity of a mesophilic family 11 xylanase: Structural basis and molecular study
17. Analysis of protein loop closure: Two types of hinges produce one motion in lactate dehydrogenase
18. Directed evolution of a thermostable esterase
19. Analysis of thermal stabilizing interactions in mesophilic and thermophilic adenylate kinases from the genus Methanococcus
20. Thermal adaptation analyzed by comparison of protein sequences from mesophilic and extremely thermophilic Methanococcus species
21. The extreme thermostable pyrophosphatase from Sulfolobus acidocaldarius: Enzymatic and comparative biophysical characterization
22. Thermoadaptation of methanogenic bacteria by intracellular ion concentration
23. Alpha-helix dipole and properties of proteins
24. Stability and stabilization of globular proteins in solution
25. Protein stability and molecular adaptation to extreme conditions
26. Investigation of the structural basis for thermostability of DNA-binding protein HU from Bacillus stearothermophilus
27. Folding funnels and binding mechanisms
28. The crystal structure of triosephosphate isomerase (TIM) from Thermotoga maritima: A comparative thermostability structural analysis of ten different TIM structures
29. New compatible solutes related to di-myo-inositol-phosphate in members of the order Thermotogales
30. Organic solutes in hyperthermophilic Archaea
31. Enhanced protein thermostability from site-directed mutations that decrease the entropy of unfolding
32. A comparative study of the thermal stability of the vertebrate eye lens: Antarctic ice fish to the desert iguana
33. Directed evolution study of temperature adaptation in a psychrophilic enzyme
34. Thermal stability and atomic-resolution crystal structure of the Bacillus caldolyticus cold shock protein
35. Subtilisin from psychrophilic Antarctic bacteria: Characterization and site-directed mutagenesis of residues possibly involved in the adaptation to cold
36. Two exposed amino acid residues confer thermostability on a cold shock protein
37. The hydrophobic effect: A reappraisal
38. Cloning and overexpression of the triosephosphate isomerase genes from psychrophilic and thermophilic bacteria: Structural comparison of the predicted protein sequences
39. Combined effect of the growth temperature and salinity of the medium on the accumulation of compatible solutes by Rhodothermus marinus and Rhodothermus obamensis
40. Proteins and temperature
41. A physicochemical basis for the selection of osmolytes by nature
42. Effect of temperature on stability and activity of elongation factor 2 proteins from Antarctic and thermophilic methanogens
43. Transproteomic evidence of a loop-deletion mechanism for enhancing protein thermostability
44. Why is one bacillus alpha-amylase more resistant against irreversible thermoinactivation than another?
45. Ensemble modulation as an origin of denaturant-independent hydrogen exchange in proteins
46. Electrostatic contributions to the stability of hyperthermophilic proteins
47. Differences in the chemical reactivity of individual molecules of an enzyme
48. How soft is a protein? A protein dynamics force constant measured by neutron scattering
49. Adjustment of conformational flexibility is a key event in the thermal adaptation of proteins