Proteins with β-(thienopyrrolyl)alanines as alternative chromophores and pharmaceutically active amino acids

Protein Science

Volumn 10, Issue 7, 2001, Pages 1281-1292

  Budisa, Nediljko ^a   Alefelder, Stefan ^a   Bae, Jae Hyun ^a   Golbik, Ralph ^b   Minks, Caroline ^a,c   Huber, Robert ^a   Moroder, Luis ^a  

^a MAX PLANCK INSTITUTE OF BIOCHEMISTRY   (Germany)

^b MARTIN LUTHER UNIVERSITY HALLE WITTENBERG   (Germany)

^c UNIVERSITY OF VIRGINIA   (United States)

Author keywords

Bioincorporation; Fluorescence; Protein engineering; Thia surrogates of tryptophan; UV absorbance

Indexed keywords

ALANINE DERIVATIVE; BARNASE; BETA (THIENOPYRROLYL)ALANINE DERIVATIVE; FLUORESCENT DYE; LIPOCORTIN 4; RECOMBINANT PROTEIN; TRYPTOPHAN DERIVATIVE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CIRCULAR DICHROISM; DRUG DESIGN; DRUG STABILITY; ENZYME INHIBITION; ESCHERICHIA COLI; FERMENTATION; FLUORESCENCE; GENETIC ENGINEERING; NONHUMAN; PHYSICAL CHEMISTRY; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN SECONDARY STRUCTURE; PROTEIN SYNTHESIS; RECOMBINANT DNA TECHNOLOGY; THERMODYNAMICS; ULTRAVIOLET SPECTROSCOPY;

ALANINE; ANNEXIN A5; BACTERIAL PROTEINS; BICYCLO COMPOUNDS, HETEROCYCLIC; CIRCULAR DICHROISM; ESCHERICHIA COLI; FERMENTATION; HEAT; HUMANS; MODELS, MOLECULAR; MOLECULAR MIMICRY; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; PROTEIN ENGINEERING; PROTEINS; TRYPTOPHAN;

ESCHERICHIA COLI; THIA;

EID: 0034973192     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.51601     Document Type: Article

References (36)

1. Structure-function analysis of the ion channel selectivity filter in human annexin V
2. A new aromaticity index and its application to five-member ring heterocycles
3. Thieno[3,2-b]- and Thieno[2,3-b]-pyrrole bioisosteric analogues of the hallucinogen and serotonin agonist N,N-dimethyltrytamine
4. Thiazole and thiophene analogues of donor-acceptor stilbenes: Molecular hyperpolarizibilities and structure-property relationships
5. Suppression of fluorescence of tryptophan residues in proteins by replacement with 4-fluorotryptophan
6. High level biosynthetic substitution of methionine in proteins by its analogues 2-aminohexanoic acid, selenomethionine, telluromethionine and ethionine in Escherichia coli
7. Atomic mutations in annexin V. Theromdynamic study of isomorphous protein variants
8. Residue-specific bioincorporation of non-natural, biologically active amino acids into proteins as possible drug carriers: Structure and stability of the per-thiaproline mutant of annexin V
9. Toward the experimental codon reassignment in vivo: Protein building with an expanded amino acid repertoire
10. Isosterism and bioisosterism in drug design
11. Remplacement total de la methionine par la selenomethionine dans les proteines d'Escherichia coli
12. 2+-induced conformational changes
13. Denatured states of proteins
14. Cation-π interactions in chemistry and biology: A new view of benzene, Phe, Tyr and Trp
15. Folding of barstar C40/C82A/ P27A and catalysis of the peptydil-prolyl cis/trans isomerisation by human cytosolic cyclophilin (Cyp 18)
16. The synthetic utility of heteroaromatic azido compounds: Preparation of some furo-, thieno-, and selenolo[3,2-b]pyrroles
17. The crystal and molecular structure of human annexin V, an anticoagulant protein that binds to calcium and membranes
18. Biosynthesis of a protein containing a nonprotein amino acid by Escherichia coli
19. Biosynthesis of a periodic protein containing 3-thienylalanine: A step toward genetically engineered conducting polymers
20. MOLSCRIPT - A program to produce both detailed and schematic plots of protein structures
21. Three-dimensional structure of annexins
22. Progress toward the evolution of an organism with an expanded genetic code
23. Room temperature phosphoroscence from tryptophan halogenated analogs in amorphous sucrose
24. X-ray structural analysis of compensating mutations at the barnase-barstar interface
25. On the aromatic stability of positional isomers consisting of bicyclic systems composed entirely of five-membered heterocycles
26. Atomic mutations at the single tryptophan residue of human recombinant annexin V: Effects on structure, stability and activity
27. Non-invasive tracing of recombinant proteins with "fluorophenylalanine-fingers."
28. Towards new protein engineering: In vivo building and folding of protein shuttles for drug delivery and targeting by the selective pressure incorporation (SPI) method
29. Folding of tryptophan mutants of barstar: Evidence for an initial hydrophobic collapse on the folding pathway
30. Enzymatic synthesis of thia-L-tryptophans
31. On the incorporation of 5′5′5′-Trifluoroleucine into protein of Escherichia coli
32. Enhancement of protein spectra with tryptophan analogues: Fluorescence spectroscopy of protein-protein and protein-nucleic acid interactions
33. The dynamic behaviour of annexin V as a function of calcium ion binding: A circular dichroism, UV absorption, and steady-state and time-resolved fluorescence study
34. Biosynthetic incorporation of 7-azatryptophan into the phage lambda lysozyme
35. Effects of α-deuteration and of aza and thia analogs of L-tryptophan and formations of intermediates on the reaction of Escherichia coli tryptophan idole-lyase
36. Aromatic and cystine side-chain circular dichroism in proteins