In vivo evidence for two active nuclease motifs in the double-strand break repair enzyme RexAB of Lactococcus lactis

Journal of Bacteriology

Volumn 183, Issue 13, 2001, Pages 4071-4078

  Quiberoni, A ^a   Biswas, I ^a   El Karoui, M ^a   Rezaiki L ^a   Tailliez, P ^a   Gruss, A ^a  

^a CEMAGREF   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL ENZYME; DOUBLE STRANDED DNA; EXONUCLEASE; NUCLEASE; PROTEIN REXAB; UNCLASSIFIED DRUG;

ARTICLE; CELL SURVIVAL; CHROMOSOME BREAKAGE; CONTROLLED STUDY; DNA REPAIR; DNA STRAND; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME INACTIVATION; ESCHERICHIA COLI; GENE FUNCTION; GENE MUTATION; LACTOBACILLUS; LACTOCOCCUS LACTIS; MOLECULAR RECOGNITION; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS;

AMINO ACID MOTIFS; AMINO ACID SEQUENCE; CATALYTIC DOMAIN; CONSERVED SEQUENCE; DNA HELICASES; DNA REPAIR; ESCHERICHIA COLI PROTEINS; EXODEOXYRIBONUCLEASE V; EXODEOXYRIBONUCLEASES; LACTOCOCCUS LACTIS; MODELS, GENETIC; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; RECOMBINATION, GENETIC; REGULATORY SEQUENCES, NUCLEIC ACID; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 0034971260     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.13.4071-4078.2001     Document Type: Article

References (54)

1. Genetic dissection of the biochemical activities of RecBCD enzyme
2. The RecD subunit of the Escherichia coli RecBCD enzyme inhibits RecA loading, homologous recombination and DNA repair
3. A single mutation; RecBD1080A, eliminates RecA protein loading but not Chi recognition by RecBCD enzyme
4. The recombination hot spot chi is a regulatory element that switches the polarity of DNA degradation by the RecBCD enzyme
5. Conserved domains in DNA repair proteins and evolution of repair systems
6. Translocation step size and mechanism of the RecBC DNA helicase
7. Codon usage can explain GT-rich islands surrounding Chi sites on the Escherichia coli genome
8. A 7-base-pair sequence protects DNA from exonucleolytic degradation in Lactococcus lactis
9. The Bacillus subtilis AddAB helicase/nuclease is regulated by its cognate Chi sequence in vitro
10. A five-nucleotide sequence protects DNA from exonucleolytic degradation by AddAB, the RecBCD analogue of Bacillus subtilis
11. Functions of the ATP hydrolysis subunits (RecB and RecD) in the nuclease reactions catalyzed by the RecBCD enzyme from Escherichia coli
12. The RecBC enzyme loads RecA protein onto ssDNA asymmetrically and independently of chi, resulting in constitutive recombination activation
13. Identification of the RecA protein-loading domain of RecBCD enzyme
14. Chi sequence protects against RecBCD degradation of DNA in viva
15. Gene replacement with linear DNA fragments in wild-type Escherichia coli: Enhancement by Chi sites
16. Role of the Escherichia coli recombination hotspot, χ, in RecABCD-dependent homologous pairing
17. High efficiency transformation of E. coli by high voltage electroporation
18. Characterization of Lactococcus lactis UV-sensitive mutants obtained by ISS1 transposition
19. Use of degenerate primers for polymerase chain reaction cloning and sequencing of the Lactococcus lactis subsp, lactis recA gene
20. Gene replacement with linear DNA in electroporated wild-type Escherichia coli
21. Characteristics of Chi distribution on different bacterial genomes
22. Identification of the lactococcal exonuclease/recombinase and its modulation by the putative Chi sequence
23. Orientation specificity of the Lactococcus lactis Chi site
24. The phylogeny of prokaryotes
25. Plasmid complements of Streptococcus lactis NCDO 712 and other lactic streptococci after protoplast-induced curing
26. Insertion of foreign DNA into plasmids from gram-positive bacteria induces formation of high-molecular-weight plasmid multimers
27. Protein phylogenies and signature sequences: A reappraisal of evolutionary relationships among archaebacteria, eubacteria, and eukaryotes
28. The C terminus of the AddA subunit of the Bacillus subtilis ATP-dependent DNase is required for the ATP-dependent exonuclease activity but not for the helicase activity
29. Orientation of cohesive end site cos determines the active orientation of chi sequence in stimulating RecA. RecBC-mediated recombination in phage lambda lytic infections
30. A conserved helicase motif of the AddA subunit of the Bacillus subtilis ATP-dependent nuclease (AddAB) is essential for DNA repair and recombination
31. The Bacillus subtilis addAB genes are fully functional in Escherichia coli
32. Biochemistry of homologous recombination in Escherichia coli
33. Recombinational repair of DNA damage in Escherichia coli and bacteriophage lambda
34. Cloning and identification of bacteriophage T4 gene 2 product gp2 and action of gp2 on infecting DNA in vivo
35. Tailed duplex DNA is the preferred substrate for Rad51 protein-mediated homologous pairing
36. DNA double-strand breaks caused by replication arrest
37. Use of bacteriophage recombination functions to promote gene replacement in Escherichia coli
38. The RecB protein of Escherichia coli translocates along single-stranded DNA in the 3′ to 5′ direction: A proposed ratchet mechanism
39. Evidence for both 3′ and 5′ single-strand DNA ends in intermediates in chi-stimulated recombination in vivo
40. Evidence that recBC-dependent degradation of duplex DNA in Escherichia coli recD mutants involves DNA unwinding
41. Inhibition of the recBCD-dependent activation of Chi recombinational hot spots in SOS-induced cells of Escherichia coli
42. Conservation of Chi cutting activity in terrestrial and marine enteric bacteria
43. RuvAB acts at arrested replication forks
44. Structure of chi hotspots of generalized recombination
45. Identification of the Chi site of Haemophilus influenzae as several sequences related to the Escherichia coli Chi site
46. Rec-mediated recombinational hot spot activity in bacteriophage lambda. III. Chi mutations are site-mutations stimulating rec-mediated recombination
47. RecBC enzyme nicking at Chi sites during DNA unwinding: Location and orientation-dependence of the cutting
48. A hypothesis: Chi-activation of RecBCD enzyme involves removal of the RecD subunit
49. Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism
50. A variant of the staphylococcal chloramphenicol resistance
51. A single nuclease active site of the Escherichia coli RecBCD enzyme catalyzes single-stranded DNA degradation in both directions
52. Functional analyses of Proteus mirabilis wild-type and mutant RecBCD enzymes in Escherichia coli reveal a new mutant phenotype
53. Identification of the nuclease active site in the multifunctional RecBCD enzyme by creation of a chimeric enzyme
54. The 30-kDa C-terminal domain of the RecB protein is critical for the nuclease activity, but not the helicase activity, of the RecBCD enzyme from Escherichia coli