The Bacillus subtilis AddAB helicase/nuclease is regulated by its cognate Chi sequence in vitro

Journal of Molecular Biology

Volumn 298, Issue 1, 2000, Pages 7-20

  Chédin, Frédéric ^a,b   Ehrlich, S Dusko ^b   Kowalczykowski, Stephen C ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b CEMAGREF   (France)

Author keywords

AddAB; Chi; Helicase; Homologous recombination; Nuclease

Indexed keywords

BACTERIAL ENZYME; DOUBLE STRANDED DNA; HELICASE; NUCLEASE;

ARTICLE; BACILLUS SUBTILIS; CONTROLLED STUDY; DNA DEGRADATION; DNA RECOMBINATION; DNA STRAND BREAKAGE; ENZYME ACTIVITY; ENZYME SUBUNIT; ESCHERICHIA COLI; GENE TRANSLOCATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN INTERACTION; SEQUENCE ANALYSIS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0034696750     PISSN: 00222836     EISSN: None     Source Type: Journal    
DOI: 10.1006/jmbi.2000.3556     Document Type: Article

References (49)

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2. The recombination hot spot χ is a regulatory element that switches the polarity of DNA degradation by the RecBCD enzyme
3. The translocating RecBCD enzyme stimulates recombination by directing RecA protein onto ssDNA in a χ-regulated manner
4. Chi-activated RecBCD enzyme possesses 5' → 3' nucleolytic activity, but RecBC enzyme does not: Evidence suggesting that the alteration induced by Chi is not simply ejection of the RecD subunit
5. Clustering of meiotic double-strand breaks on yeast chromosome III
6. A seven-base-pair sequence protects DNA from exonucleolytic degradation in Lactococcus lactis
7. A five-nucleotide sequence protects DNA from exonucleolytic degradation by AddAB, the RecBCD analogue of Bacillus subtilis
8. Recombinational hotspot activity of Chi-like sequences
9. Cutting of Chi-like sequences by the RecBCD enzyme of E. coli
10. The RecBC enzyme loads RecA protein onto ssDNA asymmetrically and independently of chi, resulting in constitutive recombination activation
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12. Homologous pairing in vitro stimulated by the recombination hotspot, Chi
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14. Role of the Escherichia coli recombination hotspot, χ, in RecABCD-dependent homologous pairing
15. Reversible inactivation of the Escherichia coli RecBCD enzyme by the recombination hotspot χ in vitro: Evidence for functional inactivation or loss of the RecD subunit
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19. Heteroduplex joint formation in Escherichia coli recombination is initiated by pairing of a 3'-ending strand
20. Effects of lysine-to-glycine mutations in the ATP-binding consensus sequences in the AddA and AddB subunits on the Bacillus subtilis AddAB enzyme activities
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22. 3' Homologous free ends are required for stable joint molecule formation by the RecA and single-stranded binding proteins of Escherichia coli
23. The preference for a 3' homologous end is intrinsic to RecA-promoted strand exchange
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26. Interaction with the recombination hot spot χ in vivo converts the RecBCD enzyme of Escherichia coli into a χ-independent recombinase by inactivation of the RecD subunit
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29. Statistical determination of the average values of the extinction coefficients of tryptophan and tyrosine in native proteins
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35. Characterization of the helicase activity of the Escherichia coli RecBCD enzyme using a novel helicase assay
36. Homologous recombination in procaryotes
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39. Rec-mediated recombinational hot spot activity in bacteriophage lambda. III. Chi mutations are site-mutations stimulating rec-mediated recombination
40. Further tests of a recombination model in which Chi removes the RecD subunit from the RecBCD enzyme of Escherichia coli
41. Double-strand breaks at an initiation site for meiotic gene conversion
42. Extensive 3'-overhanging, single-stranded DNA associated with the meiosis-specific double-strand breaks at the ARG4 recombination initiation site
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48. Identification of the nuclease active site in the multifunctional RecBCD enzyme by creation of a chimeric enzyme