Elucidation of neuronal circuitry: Protocol(s) combining intracellular labeling, neuroanatomical tracing and immunocytochemical methodologies

Brain Research Protocols

Volumn 7, Issue 3, 2001, Pages 222-234

  Luo, Pifu ^a,b   Haines, Ashley ^a   Dessem, Dean ^a  

^a UNIVERSITY OF MARYLAND   (United States)

^b UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

Biotinamide; Biotinylated dextran amine; Horseradish peroxidase; Immunocytochemistry; Intracellular labeling; Neural tracing; Ultrastructure

Indexed keywords

ANTIBODY; CHOLINE ACETYLTRANSFERASE; HORSERADISH PEROXIDASE; NICKEL; PARVALBUMIN; SODIUM; TETRAMETHYLBENZIDINE; TUNGSTEN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BIOTINYLATION; CELL LABELING; COLOR; CONTROLLED STUDY; ELECTRON MICROSCOPY; IMMUNOCYTOCHEMISTRY; MALE; METHODOLOGY; MOTONEURON; MUSCLE SPINDLE AFFERENT NERVE; NONHUMAN; PERCEPTION; PRESYNAPTIC NERVE; PRIORITY JOURNAL; RAT; TECHNIQUE;

ANIMALS; BIOTIN; CHOLINE O-ACETYLTRANSFERASE; DEXTRANS; FLUORESCENT DYES; HORSERADISH PEROXIDASE; IMMUNOHISTOCHEMISTRY; INDICATORS AND REAGENTS; MALE; MICROELECTRODES; MICROSCOPY, ELECTRON; NERVE NET; NEURONS; PARVALBUMINS; PERFUSION; RATS; RATS, SPRAGUE-DAWLEY; STAINING AND LABELING; TISSUE FIXATION;

EID: 0034960013     PISSN: 1385299X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1385-299X(01)00065-4     Document Type: Article

References (51)

1. Dendritic architecture of hypoglossal motoneurons projecting to extrinsic tongue musculature in the rat
2. Anterograde tracing of trigeminal afferent pathways from the murine tooth pulp to cortex using herpes simplex virus type 1
3. Transneuronal labelling of CNS neurons with herpes simplex virus
4. Transneuronal transport of herpes simplex virus from the cervical vagus to brain neurons with axonal input to central vagal sensory nuclei in the rat
5. Intracellular lucifer yellow injection in fixed brain slices combined with retrograde tracing, light and electron microscopy
6. Combining retrograde tracing, intracellular injection, anterograde degeneration and electron microscopy to reveal synaptic links
7. Combining laser scanning confocal microscopy and electron microscopy to determine sites of synaptic contact between two identified neurons
8. Pseudorabies virus infection of the rat central nervous system: Ultrastructural characterization of viral replication, transport and pathogenesis
9. Intracellular labeling of cat spinal neurons using a tetramethylrhodamine-dextran amine conjugate
10. C-Fos expression in the auditory pathways related to the significance of acoustic in rats performing a sensory-motor task
11. Validation of a new antiserum directed towards the synthetic c-terminus of the FOS protein in avian species: Immunological, physiological and behavioral evidence
12. Use of c-fos functional mapping to identify the central baroreceptor reflex pathway: Advantages and limitations
13. Primary- and secondary-like jaw-muscle spindle afferents have characteristic topographic distributions
14. Jaw-muscle spindle afferent feedback to the cervical spinal cord in the rat
15. C-fos expression as a marker of central cardiovascular neurons
16. Tracing of neuronal connections with cholera toxin subunit B: Light and electron microscopic immunohistochemistry using monoclonal antibodies
17. Sequential double labelling with different fluorescent dyes coupled to dextran amines as a tool to estimate the accuracy of tracer application and of regeneration
18. Electron microscopical demonstration of horseradish peroxidase by use of tetramethylbenzidine as chromogen and sodium tungstate as stablizer (TMB-ST method): A tracing method with high sensitivity and well preserved ultrastructural tissue
19. Confocal microscopic estimation of GABAergic nerve terminals in the central nervous system
20. Central activation of the trigeminovascular pathway in the cat is inhibited by dihydroergotamine. A c-Fos and electrophysiological study
21. Neurobiotin™, a useful neuroanatomical tracer for in vivo anterograde, retrograde and transneuronal tract-tracing and for in vitro labeling of neurons
22. Contacts between serotinergic fibers and dorsal horn spinocerebellar tract neurons in the cat and rat: A confocal microscopic study
23. Specificity of pseudorabies virus as a retrograde marker of sympathetic preganglionic neurons: Implications for transneuronal labeling studies
24. Combined anterograde tracing with biotinylated dextran-amine, retrograde tracing with Fast Blue and intracellular filling of neurones with Lucifer Yellow: An electron microscopic method
25. Use of peroxidase substrate Vector VIP® for multiple staining in light microscopy
26. Complex brain circuits studied via simultaneous and permanent detection of three transported neuroanatomical tracers in the same histological section
27. Inputs from identified jaw-muscle spindle afferents to trigeminothalamic neurons in the rat: A double-labeling study using retrograde HRP and intracellular biotinamide
28. Transneuronal transport of intracellularly injected biotinamide in primary afferent axons
29. Ultrastructural anatomy of physiologically identified jaw-muscle spindle afferent terminations onto retrogradely labeled jaw-elevator motoneurons in the rat
30. The morphological evidence of the 'three neuron proprioceptive pathway' of the fifth nerve in the rat - A light and electron microscopic study of ricin transganglionic degeneration combined with HRP retrograde tracing
31. Ultrastructural basis for synaptic transmission between jaw-muscle spindle afferents and trigeminothalamic neurons in the rostral trigeminal sensory nuclei of the rat
32. Serotonergic and noradrenergic axonal contacts associated with premotor interneurons in spinal pathways from group II muscle afferents
33. Sensitivity in horseradish peroxidase neurohistochemistry: A comparative and quantitative study of nine methods
34. Spatiotemporal responses of astrocytes, ramified microglia, and brain macrophages to central neuronal infection with pseudorabies virus
35. Stabilization of the tetramethylbenzidine (TMB) reaction product: Application for retrograde and anterograde tracing, and combination with immunocytochemistry
36. Comparison of cerebellothalamic and pallidothalamic projections in the monkey (Macaca fuscata): A double anterograde labeling study
37. Biotinylated dextran amine and biocytin hydrochloride are useful tracers for the study of retinal projections in the frog
38. Fluoro-Gold: A new fluorescent retrograde axonal tracer with numerous unique properties
39. Projection from the cerebellar lateral nucleus to precerebellar nuclei in the mossy fiber pathway is glutamatergic: A study combining anterograde tracing with immunogold labeling in the rat
40. 14C]2-deoxyglucose uptake in the caudal cerebellum of the rat during motor/sensory cortex stimulation
41. A fos-lac Z transgenic mouse that can be used for neuroanatomic mapping
42. Electron microscopical identification of 3,3′,5,5′-tetramethylbenzidine-reacted horseradish peroxidase after retrograde axoplasmic transport
43. A method for combining confocal and electron microscopic examination of sections processed for double- or triple-labelling immunocytochemistry
44. Specificity of rabies virus as a transneuronal tracer of motor networks: Transfer from hypoglossal motoneurons to connected second-order and higher order central nervous system cell groups
45. Many diverse types of retinal neurons show tracer coupling when injected with biocytin or Neurobiotin
46. Double-label immunocytochemistry: Combination of anterograde neuroanatomical tracing with Phaseolus vulgaris leucoagglutinin and enzyme immunocytochemistry of target neurons
47. Simultaneous characterization of efferent and afferent connectivity, neuroactive substances, and morphology of neurons
48. The anterograde neuroanatomical tracer biotinylated dextran-amine: Comparison with the tracer Phaseolus vulgaris-leucoagglutinin in preparation for electron microscopy
49. Calretinin in the entorhinal cortex of the rat: Distribution, morphology, ultrastructure of neurons, and co-localization with γ-aminobutyric acid and parvalbumin
50. Fluorescent tracers as potential candidates for double labeling of descending brain neurons in larval lamprey
51. The use of peroxidase substrate vector VIP in electron microscopic single and double antigen localization