Confocal microscopic estimation of GABAergic nerve terminals in the central nervous system

Journal of Neuroscience Methods

Volumn 95, Issue 1, 2000, Pages 1-11

  Hiscock, J J ^a   Murphy, S ^b   Willoughby, J O ^a  

^a FLINDERS UNIVERSITY   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Dentate nucleus; GAD; Hippocampus; Neocortex; Synaptophysin; Terminal area; Ultrastructure

Indexed keywords

4 AMINOBUTYRIC ACID RECEPTOR; GLUTAMATE DECARBOXYLASE; SYNAPTOPHYSIN;

ANIMAL TISSUE; ARTICLE; BRAIN REGION; CONFOCAL MICROSCOPY; DENTATE NUCLEUS; ELECTRON MICROSCOPY; GABAERGIC SYSTEM; HIPPOCAMPUS; IMMUNOCYTOCHEMISTRY; MICROSCOPE; NEOCORTEX; NERVE ENDING; NONHUMAN; PRIORITY JOURNAL; PROTEIN LOCALIZATION; RAT; VISUAL CORTEX;

ANIMALS; DENTATE GYRUS; GAMMA-AMINOBUTYRIC ACID; HIPPOCAMPUS; MICROSCOPY, CONFOCAL; PRESYNAPTIC TERMINALS; RATS; VISUAL CORTEX;

EID: 0033963618     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(99)00163-6     Document Type: Article

References (25)

1. Babb T.L., Pretorius J.K., Kupfer W.R., Jann Brown W. Distribution of glutamate-decarboxylase-immunoreactive neurons and synapses in the rat and monkey hippocampus: light and electronmicroscopy. J. Comp. Neurol. 278:1988;121-138.
2. Beaulieu C., Campistron G., Crevier C. Quantitative aspects of the GABA circuitry in the primary visual cortex of the adult rat. J. Comp. Neurol. 339:1994;559-572.
3. Calhoun M.E., Jucker M., Martin L.J., Thinakaran G., Price D.L., Mouton P.R. Comparative evaluation of synaptophysin-based methods for quantitation of synapses. J. Neurocytol. 25:1996;821-828.
4. Fykse E.M., Takei K., Walch-Solimena C., Geppert M., Jahn R., De Camilli P., Sudhof T.C. Relative properties and localizations of synaptic vesicle protein isoforms: The case of the synaptophysins. J. Neurosci. 13:1993;4997-5007.
5. Gabbot P.L., Somogyi J., Stewart M.G., Hamari J. GABA-immunoreactive neurons in the rat cerebellum: a light and electronmicroscope study. J. Comp. Neurol. 251:1986;474-490.
6. Gamrani H., Onteniente B., Seguela P., Geffard M., Calas A. Gamma-aminobutyric acid-immunoreactivity in the rat hippocampus. A light and electronmicroscopic study with anti-GABA antibodies. Brain Res. 364:1986;30-38.
7. Hennig G., Costa M., Nichols K., Brookes S.J.H. Descending cholinergic and non-cholinergic myenteric interneurons have NOS immunoreactivity in the guinea-pig small intestine. J. Autonomic Nervous Sys. 65:1997;131.
8. Jahn R., Schiebler W., Ouimet C., Greengard P. A 38,000-dalton membrane protein present in synaptic vesicles. Proc. Natl. Acad. Sci. USA. 82:1985;4137-4141.
9. Kaufman D.L., Houser C.R., Tobin A.J. Two forms of the gammaaminobutyric acid synthetic enzyme glutamate decarboxylase have distinct intraneuronal distributions and cofactor interactions. J. Neurochem. 56:1991;720-723.
10. Leclerc N., Beesley P.W., Brown I., Colonnier M., Gurd J.W., Paladino T., Hawkes R. Synaptophysin expression during synaptogenesis in the rat cerebellar cortex. J. Comp. Neurol. 280:1989;197-202.
11. Llewellyn-Smith I.J., Minson J.B., Pilowsky P.M. Retrograde tracing with cholera toxin B-gold or with immunocytochemically detected cholera toxin B in central nervous system. Meth. Neurosci. 8:1992;180-201.
12. Masliah E., Terry R.D., Alford M., DeTeresa R. Quantitative immunohistochemistry of synaptophysin in human neocortex: An alternative method to estimate density of presynaptic terminals in paraffin sections. J. Histochem. Cytochem. 38:1990;837-844.
13. McLaughlin B.J., Wood J.G., Saito K., Barber R., Vaughn J.E., Roberts E, Wu J. The fine structural localization of glutamate decarboxylase in synaptic terminals of rodent cerebellum. Brain Res. 76:1974;377-391.
14. McLaughlin B.J., Wood J.G., Saito K., Barber R., Vaughn J.E., Roberts E, Ribak R. Aspinous and sparsely-spinous stellate neurons in the visual cortex of rats contain glutamic acid decarboxylase. J. Neurocytol. 7:1978;461-478.
15. Mouton P.R., Price D.L., Walker L.C. Empirical assessment of synapse numbers in primate neocortex. J. Neurosci. Meth. 75:1997;119-126.
16. Mugnaini E., Oertel W.H. An atlas of the distribution of GABA-ergic neurons and terminals in the rat CNS as revealed by GAD immunohistochemistry. Bjorklund A., Hokfelt T. GABA and Neuropeptides in the CNS. 1985;436-608 Elsevier, Amsterdam.
17. Nemoto T., Konno A., Chiba T. Synaptic contact of neuropeptide- and amine-containing axons on parasympathetic preganglionic neurons in the superior salivatory nucleus of the rat. Brain Res. 685:1995;33-45.
18. Niedermeyer E. Epileptic seizure disorders. Neidermeyer E., Lopes da Silva F. Electroencephalography: basic principles, clinical applications and related fields. 1982;339-428 Urban & Schwarzenberg, Baltimore, USA.
19. Paxinos G., Watson C. The Rat Brain in Stereotaxic Coordinates. 1986;Paxinos, Sydney.
20. Peters A., Harriman K. Different kinds of axon terminals forming symmetric synapses with the cell bodies and initial axon segments of layer II/III pyramidal cells. I. Morphometric analysis. J. Neurocytol. 19:1990;154-174.
21. Sandmann D., Engelmann R., Peichl L. Starburst cholinergic amacrine cells in the tree shrew retina. J. Comp. Neurol. 389:1997;161-176.
22. Shinoda Y., Suguichi Y., Futami T. Organisation of excitatory inputs from the cerebral cortex to the cerebellar dentate nucleus. Can. J. Neurol. Sci. 3:1993;19-S28.
23. Sudhof T.C., Jahn R. Proteins of synaptic vesicles involved in exocytosis and membrane recycling. Neuron. 6:1991;665-677.
24. Sze C., Troncoso J.T., Kawas C.H., Mouton P.R., Price D.L., Martin L.J. Loss of presynaptic vesicle protein synaptophysin in hippocampus correlates with early cognitive decline in aged humans. Soc. Neurosci. 22:1996;199.
25. Wiedenmann B., Franke W.W. Identification and localization of synaptophysin, an intergral membrane glycoprotein of M 38,000 characteristic of presynaptic vesicles. Cell. 41:1985;1017-1028.