A method for combining confocal and electron microscopic examination of sections processed for double- or triple-labelling immunocytochemistry

Journal of Neuroscience Methods

Volumn 73, Issue 2, 1997, Pages 149-157

  Todd, A J ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Confocal laser scanning microscopy; Electron microscopy; Neurokinin 1 receptor; Spinal cord; Substance P

Indexed keywords

CALCITONIN GENE RELATED PEPTIDE; DIAMINOBENZIDINE; NEUROKININ 1 RECEPTOR; SUBSTANCE P;

ANIMAL TISSUE; ARTICLE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ELECTRON MICROSCOPY; IMMUNOCYTOCHEMISTRY; IMMUNOPEROXIDASE STAINING; MALE; NONHUMAN; PRIORITY JOURNAL; RAT; SPINAL CORD DORSAL HORN; SYNAPSE;

ANIMALS; CALCITONIN GENE-RELATED PEPTIDE; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOENZYME TECHNIQUES; IMMUNOHISTOCHEMISTRY; MALE; MICROSCOPY, CONFOCAL; MICROSCOPY, ELECTRON; NEUROSCIENCES; RATS; RATS, INBRED STRAINS; RECEPTORS, NEUROKININ-1; SPINAL CORD; STAINING AND LABELING; SUBSTANCE P; SYNAPSES;

EID: 0030985172     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(97)02222-X     Document Type: Article

References (22)

1. Balercia, G., Chen, S. and Bentivoglio, M. (1992) Electron microscopic analysis of fluorescent neuronal labelling after photoconversion. J. Neurosci., Methods, 45: 87-98.
2. Bleazard, L., Hill, R.G. and Morris, R. (1994) The correlation between the distribution of NK1 receptor and the actions of tachykinin agonists in the dorsal horn of the rat indicates that substance P does not have a functional role on substantia gelatinosa (lamina II) neurons. J. Neurosci., 14: 7655-7664.
3. Bolam, J.P. (1992) Preparation of central nervous system tissue for light and electron microscopy. In Bolam J.P. (Ed.), Experimental Neuroanatomy: A Practical Approach, IRL Press, Oxford, pp. 1-30.
4. Brelje, T.C., Wessendorf, M.W. and Sorenson, R.L. (1993) Multicolor laser scanning confocal immunofluorescence microscopy: practical application and limitation. In Matsumoto B.(Ed.), Cell Biological Applications of Confocal Microscopy, Academic Press, San Diego, pp. 98-181.
5. Brown, J.L., Liu, H., Maggio, J.E., Vigna, S.R., Mantyh, P.W. and Basbaum, A.I. (1995) Morphological characterization of substance P receptor-immunoreactive neurons in rat spinal cord and trigeminal nucleus caudalis. J. Comp. Neurol., 356: 327-344.
6. Chan, J., Aoki, C. and Pickel, V.M. (1990) Optimization of differential immunogold-silver and peroxidase labeling with maintenance of ultrastructure in brain sections before plastic embedding. J. Neurosci. Methods, 33: 113-127.
7. Ju, G., Hökfelt, T., Brodin, E., Fahrenkrug, J., Fischer, J.A., Frey, P., Eide, R.P. and Brown, J.C. (1987) Primary sensory neurons of the rat showing calcitonin gene-related peptide immunoreactivity and their relation to substance P-, somatostatin-, galanin-, vasoactive intestinal polypeptide- and cholecystokinin-immunoreactive ganglion cells. Cell Tissue Res., 247: 417-431.
8. Kosaka, T., Nagatsu, I., Wu, J.-Y. and Hama, K. (1986) Use of high concentrations of glutaraldehyde for immunocytochemistry of transmitter-synthesizing enzymes in the central nervous system. Neuroscience, 18: 975-990.
9. Littlewood, N.K., Todd, A.J., Spike, R.C., Watt, C. and Shehab, S.A.S. (1995) The types of neuron in spinal dorsal horn which possess neurokinin-1 receptors. Neuroscience, 66: 597-608.
10. Liu, H., Brown, J.L., Jasmin, L., Maggio, J.E., Vigna, S.R., Mantyh, P.W. and Basbaum, A.I. (1994) Synaptic relationship between substance P and the substance P receptor: light and electron microscopic characterization of the mismatch between neuropeptides and their receptors. Proc. Natl. Acad. Sci., USA, 91: 1009-1013.
11. Llewellyn-Smith, I.J. and Minson, J.B. (1992) Complete penetration of antibodies into Vibratome sections after glutaraldehyde fixation and ethanol treatment: light and electron microscopy for neuropeptides. J. Histochem. Cytochem., 40: 1741-1749.
12. Mantyh, P.W., DeMaster, E., Malhotra, A., Ghilardi, J.R., Rogers, S.D., Mantyh, C.R., Liu, H., Basbaum, A.I., Vigna, S.R., Maggio, J.E. and Simone, D.A. (1995) Receptor endocytosis and dendrite reshaping in spinal neurons after somatosensory stimulation. Science, 268: 1629-1632.
13. Marshall, G.E., Shehab, S.A.S., Spike, R.C. and Todd, A.J. (1996) Neurokinin 1 receptors on lumbar spinothalamic neurons in the rat. Neuroscience, 72: 255-263.
14. Nakaya, Y., Kaneko, T., Shigemoto, R., Nakanishi, S. and Mizuno, N. (1994) Immunohistochemical localization of substance P receptor in the central nervous system of the adult rat. J. Comp. Neurol., 347: 249-274.
15. Pickel, V.M., Chan, J. and Aoki, C. (1993) Electron microscopic immunocytochemical labelling of endogenous and/or transported antigens in rat brain using silver-intensified one-nanometre colloidal gold. In Cuello A.C. (Ed.), Immunohistochemistry II, Wiley and Sons, Chichester, pp. 263-280.
16. Ribeiro-da-Silva, A., Priestley, J.V. and Cuello, A.C. (1993) Pre-embedding ultrastructural immunocytochemistry. In Cuello A.C. (Ed.), Immunohistochemistry II, Wiley and Sons, Chichester, pp. 181-228.
17. Sandell, J.H. and Masland, R.H. (1988) Photoconversion of some fluorescent markers to a diaminobenzidine product. J. Histochem. Cytochem., 36: 555-559.
18. Todd, A.J. and Spike, R.C. (1993) The localization of classical transmitters and neuropeptides within neurons in laminae I-III of the mammalian spinal dorsal horn. Prog. Neurobiol., 41: 609-646.
19. Todd, A.J., Watt, C., Spike, R.C. and Sieghart, W. (1996) Co-localisation of GABA, glycine and their receptors at synapses in the rat spinal cord. J. Neurosci., 16: 974-982.
20. Totterdell, S., Ingham, C.A. and Bolam, J.P. (1992) Immunocytochemistry I: pre-embedding staining. In Bolam J.P.(Ed.), Experimental Neuroanatomy. A practical approach, IRL Press, Oxford, pp. 103-128.
21. Vigna, S.R., Bowden, J.J., McDonald, D.M., Fisher, J., Okamoto, A., McVey, D.C., Payan, D.G. and Bunnett, N.W. (1994) Characterization of antibodies to the rat substance P (NK-1) receptor and to a chimeric substance P receptor expressed in mammalian cells. J. Neurosci., 14: 834-845.
22. Willis, W.D. and Coggeshall, R.E. (1991) Sensory mechanisms of the spinal cord, 2nd ed., Wiley, New York.