Aspergillus fumigatus CYP51 sequence: Potential basis for fluconazole resistance

Medical Mycology

Volumn 39, Issue 3, 2001, Pages 299-302

  Edlind, T D ^a   Henry, K W ^a   Metera, K A ^a   Katiyar, S K ^a  

^a DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

Author keywords

Aspergillus fumigatus; Azoles; Candida albicans; CYP51

Indexed keywords

ALANINE; ANTIFUNGAL AGENT; CYTOCHROME P450; FLUCONAZOLE; PYRROLE DERIVATIVE; STEROL 14ALPHA DEMETHYLASE; THREONINE;

ANALYSIS; ANTIBIOTIC RESISTANCE; ARTICLE; ASPERGILLUS FUMIGATUS; CANDIDA ALBICANS; CLONE; COMPARATIVE STUDY; FUNGAL GENETICS; FUNGUS MUTATION; GENE SEQUENCE; HYDROGEN BOND; HYPOTHESIS; NONHUMAN; NUCLEOTIDE SEQUENCE;

ASPERGILLUS FUMIGATUS; CANDIDA ALBICANS; FUNGI; MYXOGASTRIA;

EID: 0034955638     PISSN: 13693786     EISSN: None     Source Type: Journal    
DOI: 10.1080/mmy.39.3.299.302     Document Type: Article

References (14)

1. Vanden Bossche J, Dromer F, Improvisi I, Lozano-Chiu M, Rex JH, Sanglard D. Antifungal drug resistance in pathogenic fungi. Med Mycol 1998; 36 (Suppl. 1): 119-128.
2. White TC, Marr KA, Bowden RA. Clinical, cellular, and molecular factors that contribute to antifungal drug resistance. Clin Microbiol Rev 1998; 11: 382-402.
3. Latgé J-P. Aspergillus fumigatus and aspergillosis. Clin Microbiol Rev 1999; 12: 310-350.
4. Warnock DW Fungal infections in neutropenia: current problems and chemotherapeutic control. / Antimicrob Chemother 1998; 41 (Suppl. D): 95-105.
5. Denning DW, Venkateswarlu K, Oakley KL, et al. Itraconazole resistance in Aspergillus fumigatus. Antimicrob Agents Chemother 1997; 41: 1364-1368.
6. Lai MH, Kirsch DR. Nucleotide sequence of cytochrome P450 LIAI (lanosterol 14a-demethylase) from Candida albicans. Nucleic Acids Res 1989; 17: 804.
7. Davis LG, Kuehl WM, Battey JF. Basic Methods in Molecular Biology, 2nd edn. Norwalk, CN.: Appleton & Lange, 1994.
8. Van Nistelrooy JGM, van den Brink JM, van Kan JAL, van Gorcom RFM, de Waard MA. Isolation and molecular characterization of the gene encoding eburicol 14a-demethylase (CYP51) from Pénicillium italicum. Mol Gen Genet 1996; 250: 725-733.
9. Ochman H, Medhora MM, Garza D, Hartl DL. Amplification of flanking sequences by inverse PCR. In: PCR Protocols, Innis MA, Gelfand DH, Sninsky JJ, White TJ, eds. San Diego: Academic Press, 1990: 219-227.
10. Marichal P, Koymans L, Willemsens S, et al. Contribution of mutations in the cytochrome P450 14a-demethylase (Ergllp, CypSlp) to azole resistance in Candida albicans. Microbiology 1999; 145: 2701-2713.
11. Lamb DC, Kelly DE, Schunck W-H, et al. The mutation T315A in Candida albicans sterol 14a-demethylase causes reduced enzyme activity and fluconazole resistance through reduced affinity. J Biol Chem 1997; 272: 5682-5688.
12. Boscott PE, Grant GH. Modeling cytochrome P450 14ademethylase (Candida albicans) from P450cam. / Mol Graph 1994; 12: 185-192.
13. Sanglard D, Ischer F, Koymans L, Bille J. Amino acid substitutions in the cytochrome P-450 lanosterol 14a-demethylase (CYP51A1) from azole-resistant Candida albicans clinical isolates contribute to resistance to azole antifungal agents. Antimicrob Agents Chemother 1998; 42: 241-253.
14. Loffler J, Kelly SL, Hebart H, Schumacher U, Lass-Florl C, Einsele H. Molecular analysis of cyp51 from fluconazoleresistant Candida albicans. F EM S Microbiol Lett 1997; 151: 263-268.