Purification and kinetic characterization of CTP:phosphocholine cytidylyltransferase from saccharomyces cerevisiae

Protein Expression and Purification

Volumn 21, Issue 1, 2001, Pages 141-148

  Friesen, Jon A ^a   Seo Park, Young ^a   Kent, Claudia ^a  

^a UNIVERSITY OF MICHIGAN HEALTH SYSTEM   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACULOVIRUS EXPRESSION SYSTEM; CHOLINE PHOSPHATE CYTIDYLYLTRANSFERASE; CONCENTRATION (PARAMETERS); ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME REGULATION; FUNGAL ENZYME; GENE TAGGING; GENOMIC DNA; HISTIDINE; LIPID BINDING PROTEIN; MAMMAL CELL; PHOSPHATIDYLCHOLINE; PHOSPHORYLCHOLINE; POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 0034951395     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1354     Document Type: Article

References (35)

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14. In vitro studies of phospholipid biosynthesis in Saccharomyces cerevisiae
15. Yeast mutant defective in phosphatidylserine synthesis
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17. Comparison of the lipid regulation of yeast and rat CTP:phosphocholine cytidylyltransferase expressed in COS cells
18. Molecular cloning and characterization of the gene encoding cholinephosphate cytidylyltransferase in Saccharomyces cerevisiae
19. Expression in Escherichia coli of the Saccharomyces cerevisiae CCT gene encoding cholinephosphate cytidylyltransferase
20. DNA sequencing with chain-terminating inhibitors
21. Rapid high-efficiency site-directed mutagenesis by the phosphorothioate approach
22. Expression, purification, and characterization of CTP:glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis
23. Multiple antigenic peptide method for producing antipeptide site-specific antibodies
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27. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
28. Using the Saccharomyces Genome Database (SGD) for analysis of protein similarities and structure
29. Sequencing of a 17.6 kb segment on the right arm of yeast chromosome VII reveals 12 ORFs, including CCT, ADE3 and TR-I genes, homologues of the yeast PMT and EF1G genes, of the human and bacterial electron-transferring flavoproteins (beta-chain) and of the phosphoserine phosphohydrolase, and five new ORFs
30. Suppression of the negative effect of minor arginine codons on gene expression: Preferential usage of minor codons within the first 25 codons of the Escherichia coli genes
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34. A prototypical cytidylyltransferase:CTP:glycerol-3-phosphate cytidylyltransferase from Bacillus subtilis