The theoretical relationship of immunogold labeling on acrylic sections and epoxy sections

Micron

Volumn 27, Issue 3-4, 1996, Pages 193-201

  Brorson, Sverre Henning ^a   Skjørten, Fredrik ^a  

^a ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

Author keywords

acrylic resin; epoxy resin; immunocytochemistry; immunoelectron microscopy immunogold; LR White

Indexed keywords

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0030158746     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/0968-4328(96)00034-0     Document Type: Article

References (9)

1. Brorson, S. H. and Skjørten, F., 1995. Mechanism for antigen detection on deplasticized epoxy sections. Micron, 26, 301-310.
2. Brorson, S. H., Roos, N. and Skjørten, F., 1994. Antibody penetration into LR-White sections. Micron, 25, 453-460.
3. Holm, R., Nesland, J. M., Attramadal, A. and Johannessen, J. V., 1988. Double-staining methods at the ultrastructural level applying colloidal gold conjugates. Ultrastruct. Path., 12(3), 279-290.
4. Kellenberger, E., Dürrenberger, M., Villiger, W., Carlemalm, E. and Wurtz, M., 1987. The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions. J. Histochem. Cytochem., 35, 959-969.
5. Linke, R. P., Huhn, D., Casanova, S. and Donini, U., 1989. Immunoelectron microscopic identification of human AA type amyloid: Exploration of various monoclonal AA-antibodies, methods of fixation, embedding and of other parameters for the protein A gold method. Lab. Invest., 61, 691-697.
6. Newman, G. R., 1989. LR-White embedding medium for colloidal gold methods. In: Colloidal Gold: Principles, Methods and Applications Vol. 2. Hayat M. A. (ed.). Academic Press, pp. 47-73.
7. Newman, G. R., 1987. Use and abuse of LR-White. Histochem. J., 19, 118-120.
8. Newman, G. R. and Hobot, J. A., 1987. Modern acrylics for postembedding immunostaining techniques. J. Histochem. Cytochem., 35, 971-981.
9. Skjørten, F. J., Brorson, S. H., Roald, B., Strøm, E. H. and Lund, B., 1992. The use of postembedding immunoelectron microscopy in the diagnosis of glomerular diseases. APMIS, 100, 1001-1007.