A comparative study of the immunogold labeling on H2O2-treated and heated epoxy sections

Micron

Volumn 32, Issue 2, 2001, Pages 147-151

  Brorson, S H ^a   Hansen, A R ^b   Nielsen, H Z ^b   Woxen, I K ^b  

^a ULLEVÅL UNIVERSITY HOSPITAL   (Norway)

^b OSLO METROPOLITAN UNIVERSITY   (Norway)

Author keywords

Antigen retrieval; Electron microscopy; Epoxy resin; Hydrogen peroxide; Immunoelectron microscopy; Immunogold

Indexed keywords

ANIMALIA; GLOMERULUS; SUS SCROFA;

EID: 0035239167     PISSN: 09684328     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0968-4328(99)00107-9     Document Type: Article

References (20)

1. Bovine serum albumin (BSA) as a reagent against non-specific immunogold labeling on LR-white and epoxy resin
2. Comparison of the immunogold labeling of single light chains and whole immunoglobulins with anti-κ on LR-white and epoxy sections
3. Antigen detection on resin sections and methods for improving the immunogold labeling by manipulating the resin
4. The combination of high-accelerator epoxy resin and antigen retrieval to obtain more intense immunolabeling on epoxy sections than on LR-white sections for large proteins
5. The fixative-dependent increase in immunogold labeling following antigen retrieval on acrylic and epoxy sections
6. The theoretical relationship of immunogold labeling on acrylic sections and epoxy sections
7. Improved technique for immunoelectron microscopy. How to prepare epoxy resin to obtain approximate the same immunogold labeling for epoxy sections as for acrylic sections without any etching
8. Increased yield of immunogold labeling of epoxy sections by adding para-phenylendiamine in the tissue processing
9. Antigen retrieval on epoxy sections based on tissue infiltration with moderately increased amount of accelerator to detect immune complex deposits in glomerular tissue
10. Antibody penetration into LR-white sections
11. Polar or apolar lowicryl resin for immunolabeling
12. Resin embedding for quantitative immunoelectron microscopy. A comparative computerized image analysis
13. Comparasion of LR white and Unicryl as embedding media for light and electron immunomicroscopy of chromaffin cells
14. Ultrastructural and electron immunohistochemical features of medullary thyroid carcinoma
15. The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions
16. LR-White embedding medium for colloidal gold methods
17. Quantitative electron microscopical immunocytochemistry of neuroactive aminoacids
18. Use of hydrogen peroxide to accelerate staining of ultrathin spurr sections
19. Antigen unmasking for immunoelectron microscopy: Labeling is improved by treating with sodium ethoxide or sodium metaperiodate, then heating on retrieval medium
20. Antigen retrieval for electron microscopy using a microwave technique for epithelial and basal lamina antigens