High-performance liquid chromatographic separation of natural and synthetic desulphoglucosinolates and their chemical vadation by UV, NMR and chemical ionisation-MS methods

Phytochemical Analysis

Volumn 12, Issue 4, 2001, Pages 226-242

  Kiddle, Guy ^a,c   Bennett, Richard N ^b   Botting, Nigel P ^a   Davidson, Nicola E ^a   Robertson, Avril A B ^b   Wallsgrove, Roger M ^a  

^a ROTHAMSTED RESEARCH   (United Kingdom)

^b INSTITUTE OF FOOD RESEARCH   (United Kingdom)

^c UNIVERSITY OF ST ANDREWS   (United Kingdom)

Author keywords

Chemical synthesis; Desulphoglucosinolates; Extraction; Glucosinolates; Mass spectrometry; Nuclear magnetic resonance spectroscopy; Reverse phase high performance liquid chromatography

Indexed keywords

BINARY ALLOYS; EXTRACTION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IONIZATION; MASS SPECTROMETRY;

CHEMICAL IONIZATION; DESULFATION; DESULFO-GLUCOSINOLATE; EXTRACTION/SEPARATION; GLUCOSINOLATES; HPLC SEPARATION; LIQUID CHROMATOGRAPHIC SEPARATIONS; PERFORMANCE; REVERSE PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; UV IONIZATION;

NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY;

DESULFOGLUCOSINOLATE; GLUCOSINOLATE; NATURAL PRODUCT; PLANT EXTRACT; UNCLASSIFIED DRUG;

ARTICLE; BRASSICA; CARBON NUCLEAR MAGNETIC RESONANCE; CHEMICAL ANALYSIS; CHEMICAL COMPOSITION; CHEMICAL STRUCTURE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DESULFURIZATION; EXTRACTION; MASS SPECTROMETRY; METHODOLOGY; PHASE SEPARATION; PLANT; PROTON NUCLEAR MAGNETIC RESONANCE; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; STRUCTURE ANALYSIS; SYNTHESIS; ULTRAVIOLET RADIATION; VALIDATION PROCESS;

EID: 0034927357     PISSN: 09580344     EISSN: None     Source Type: Journal    
DOI: 10.1002/pca.589     Document Type: Article

References (58)

1. High performance liquid-phase separation of glycosides: Analytical and micro-preparative HPLC combined with spectroscopic and enzymatic methods for generating a glucosinolate library
2. The palladium test and the thymol test for the analysis of glucosinolates in green matter of rape (Brassica napus L.)
3. Effects of wounding on glucosinolates in the cotyledons of oilseed rape and mustard
4. Potent effects of jasmonates on indole glucosinolates in oilseed rape and mustard
5. Structure-activity relationships of cyclopentanone analogues of jasmonic acid for induced responses of canola seedlings
6. The myrosinase-glucosinolate system, its organisation and biochemistry
7. Isolation of intact glucosinolates by column chromatography and determination of their purity
8. Separation of desulphoglucosinolates by micellar electrokinetic capillary chromatography based on a bile salt
9. Exploring an alternative approach to the synthesis of arylalkyl and indolylmethyl glucosinolates
10. Biological affects of glucosinolates
11. 13C) of glucosinolates
12. Glucosinolate composition of seeds from 297 species of wild plants
13. Selective induction of glucosinolates in oilseed rape leaves by methyl jasmonate
14. Glucosinalbin in Brassica napus-a re-evaluation
15. Glucosinolates and their breakdown products in food and food plants
16. Glucosinolates of Lepidium sativum and 'garden cress'
17. Evaluation of liquid chromatography-atmospheric pressure chemical ionisation-mass spectrometry for the identification and quantification of desulphoglucosinolates
18. Enzyme linked immunosorbent assays for alkenyl glucosinolates
19. Methods for glucosinolate analysis
20. Improved method for the determination of total glucosinolate content of rapeseed by determination of enzymatically released glucose
21. Specific screening methods for glucosinolates in sprout extracts using online thermospray liquid-chromatography tandem mass-spectroscopy
22. HPLC separation of glucosinolates from leaves and seeds of Arabidopsis thaliana and their detection using thermospray liquid-chromatography mass-spectroscopy
23. Glucosinolate content of common Brassicaceae family vegetables
24. Separation and identification of desulphoglucosinolates in Japanese rapeseed by LC/APCI-MS
25. Determination of glucosinolates using their alkaline degradation and reaction with ferricyanide
26. Total isothiocyanate contents in cooked vegetables frequently consumed in Singapore
27. High performance liquid phase separation of glycosides. II. Capillary electrophoresis of the fluorescently labelled acid hydrolysis products of glucosinolates-profiling of glucosinolates in white and red cabbages
28. High-performance liquid chromatographic method for the separation and quantification of intact glucosinolates
29. Salicylic acid-induced accumulation of glucosinolates in oilseed rape (Brassica napus L.) leaves
30. Identification of intact glucosinolates using direct coupling of high performance liquid-chromatography with continuous-flow frit fast-atom bombardment tandem mass-spectroscopy
31. HPLC-analysis of intact glucosinolates of vegetable Brassicaceae and their enzymatic detection by myrosinase degradation
32. Local and systemic changes in glucosinolates in Chinese and European cultivars of oilseed rape (Brassica napus L.) after inoculation with Sclerotinia sclerotiorum (stem rot)
33. The host specificity of Plasmodiophora brassicae and its relation to the endogenous glucosinolate content of the host plant
34. Indole glucosinolates and auxin biosynthesis in Arabidopsis thaliana glucosinolate mutants and the development of clubroot disease
35. Volatile flavour components of garden cress
36. Simultaneous determination of isothiocyanates, indoles, and oxazolidinethiones in myrosinase digests of rapeseeds and rapeseed meal by HPLC
37. Studies of mustard oil glucosides
38. Glucosinolates of wild and cultivated Brassica species
39. Quantitative analysis of individual glucosinolates in double low oilseed rape by HPLC of intact glucosinolates
40. Sinalbin and other glucosinolates in seeds of double low rape species and Brassica napus cv. Brownowski
41. Variation in the glucosinolate content of oilseed rape (Brassica napus L.) leaves. l. Effect of leaf age and position
42. Comprehensive chromatographic and spectroscopic methods for separation and identification of intact glucosinolates
43. A new approach to the study of glucosinolates by isocratic liquid chromatography. 1. Rapid determination of desulphated derivatives of rapeseed glucosinolates
44. Synthesis of deuterium labelled desulphoglucosinolates as internal standards for LC-MS analysis
45. Glucosinolates in crop plants
46. A method for the rapid and simple determination of total glucosinolate content in vegetative tissue and seeds of cruciferous crops by quantitative analysis of enzymatically released sulphate
47. Determination of total glucosinolate content in vegetative plant material by quantitative analysis of enzymatically released sulphate
48. Quantitative glucosinolate analysis in Brassica seeds by X-ray fluorescence spectroscopy
49. Identification of glucosinolates in radish (Raphanus sativus L.) and cross-breeds of R. sativus × Brassica oleracea L. (Raphanobrassica) by LC-MS
50. Separation and identification of glucosinolates in Brassica vegetables using negative-ion chemical ionisation capillary GC-MS and GC-MS-MS
51. Separation and identification of glucosinolates from Brassica vegetables using high-performance capillary gas-chromatography (GC)-positive-ion chemical ionisation mass-spectroscopy (PICIMS) and GC-PICIMS MS
52. The thymol method for glucosinolate determination
53. Quantitative determination of the glucosinolates sinigrin and progoitrin by specific antibody ELISA assays in Brussels sprouts
54. Analysis of total glucosinolate content and individual glucosinolates in Brassica ssp. by near-infrared reflectance spectroscopy
55. Rapid gaschromatographic determination of volatile degradation products of glucoisnolates in rapeseed oil
56. Synthetic studies on indole glucosinolates. Part 1. Synthesis of glucobrassicin and its 4- and 5-methoxy derivatives
57. Glucosinolates
58. Determination of glucosinolates in domestic and wild mustard by high-performance liquid chromatography with confirmation by electrospray mass spectroscopy and photo-diode array detection