The physiological effects and metabolic alterations caused by the expression of Rhizobium etli pyruvate carboxylase in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 56, Issue 1-2, 2001, Pages 188-195

  Gokarn, R R ^a   Evans, J D ^a   Walker, J R ^a   Martin, S A ^a   Eiteman, M A ^a   Altman, E ^a  

^a University of Georgia   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCOSE; HOLOENZYME; LACTATE DEHYDROGENASE; OXALOACETIC ACID; PHOSPHOENOLPYRUVATE CARBOXYLASE; PYRUVATE CARBOXYLASE; SUCCINIC ACID; TRICARBOXYLIC ACID;

MICROORGANISM;

ANAEROBIC METABOLISM; ARTICLE; BACTERIAL GROWTH; BIOMASS; BIOSYNTHESIS; BIOTINYLATION; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; GLUCOSE TRANSPORT; GROWTH RATE; NONHUMAN; PROTEIN EXPRESSION; RHIZOBIUM ETLI;

ACETATES; AEROBIOSIS; ANAEROBIOSIS; ESCHERICHIA COLI; L-LACTATE DEHYDROGENASE; PYRUVATE CARBOXYLASE; RECOMBINANT PROTEINS; RHIZOBIUM; SUCCINIC ACID;

EID: 0034918307     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530100661     Document Type: Article

References (42)

1. S gene product: Identification and membrane localization of a lysis control protein
2. Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli
3. The structure and the mechanism of action of pyruvate carboxylase
4. Genetic and biochemical characterization of the birA gene and its products: Evidence for a direct role of biotin holoenzyme synthase in repression of biotin operon in Escherichia coli
5. Improved expression of human interleukin-2 in high-cell-density fermentation cultures of Escherichia coli K-12 by a phosphotransacetylase mutant
6. The ldhA gene encoding the fermentative lactate dehydrogenase of Escherichia coli
7. Membrane protein variations associated with in vitro passage of Borrelia burgdorferi
8. Effects of adenosine phosphates and nicotinamide nucleotides on pyruvate carboxylase from baker's yeast
9. Alteration of growth yield by overexpression of phosphoenolpyruvate carboxylase and phosphoenolpyruvate carboxykinase in Escherichia coli
10. Expression of the biotin biosynthetic operon of Escherichia coli is regulated by the rate of protein biotination
11. Inverse flux analysis for reduction of acetate excretion in Escherichia coli
12. Effect of alteration of the acetic acid synthesis pathway on the fermentation pattern of Escherichia coli
13. Pyruvate carboxylase from Rhizobium etli: Mutant characterization, nucleotide sequence, and physiological role
14. Optimization of the ion-exchange analysis of organic acids from fermentation
15. Reduction of aerobic acetate production by Escherichia coli
16. Pyruvate carboxylase of Aspergillus niger: Kinetic study of a biotin-containing carboxylase
17. Expression of PEP carboxylase from Escherichia coli complements the phenotypic effects of pyruvate carboxylase mutations in Saccharomyces cerevisiae
18. Identification of a sex-factor-affinity site in E. coli as γδ
19. Pyruvate formate-lyase-deactivase and acetyl-CoA reductase activities of Escherichia coli reside on a polymeric protein particle encoded by adhE
20. Ultrastructure and pyruvate fromate-lyase radical quenching property of the multienzymic AdhE protein of Escherichia coli
21. Acetyl-CoA carboxylase from Escherichia coli: Gene organization and nucleotide sequence of the biotin carboxylase subunit
22. The role and control of the glyoxylate cycle in Escherichia coli
23. Pyruvate-formate lyase of Escherichia coli: The acetyl-enzyme intermediate
24. Pyruvate carboxylase from thermophilic Bacillus: Some molecular characteristics
25. Protein measurement with the folin phenol reagent
26. Properties of a mutant Escherichia coil phosphoenolpyruvate carboxylase deficient in coregulation by intermediary metabolites
27. Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli
28. Some properties of the pyruvate carboxylase from Pseudomonas fluorescens
29. Acetyl-CoA-dependent pyruvate carboxylase from the photosynthetic bacterium Rhodobacter capsulatus: Rapid and efficient purification using dye-ligand affinity chromatography
30. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis
31. Pyruvate carboxylase in Rhodopseudomonas spheroides
32. Pyruvate carboxylase from Corynebacterium glutamicum: Characterization, expression and inactivation of the pyc gene
33. Energetics of the bacterial phosphotransferase system in sugar transport and regulation of carbon metabolism
34. - levels and pH on growth, succinate production and enzyme activities of Anaerobiospirillum succiniproducens
35. Anaerobic regulation of pyruvate formate-lyase from Escherichia coli K-12
36. Isolation and characterization of pyruvate carboxylase from Azotobacter vinelandii OP
37. Integration host factor is required for anaerobic pyruvate induction of pfl operon expression in Escherichia coli
38. Kinetics of Escherichia coli B D-lactate dehydrogenase and evidence for pyruvate-controlled change in conformation
39. Chemical characterization of D-lactate dehydrogenase from Escherichia coli B
40. Site directed mutagenesis of phosphoenolpyruvate carboxylase from E. coli; the role of his-579 in the catalytic and regulatory functions
41. Construction and characterization of a highly regulable expression vector, pLAC11, and its multipurpose derivatives, pLAC22 and pLAC33