In situ reverse transcriptase-nested polymerase chain reaction to identify intracellular nucleic acids without the necessity of DNAse pretreatment and hybridisation

Analytical Cellular Pathology

Volumn 22, Issue 3, 2001, Pages 151-158

  Menschikowski, M ^a   Vogel, M ^a   Eckey, R ^a   Dinnebier, G ^a   Jaross, W ^a  

^a DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

DEOXYRIBONUCLEASE; MESSENGER RNA; NUCLEIC ACID; PHOSPHOLIPASE A2;

ARTICLE; CONTROLLED STUDY; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SOUTHERN BLOTTING;

EID: 0034914416     PISSN: 09218912     EISSN: None     Source Type: Journal    
DOI: 10.1155/2001/654016     Document Type: Article

References (19)

1. Intracellular amplification of proviral DNA in tissue sections using the polymerase chain reaction
2. Single step method of RNA isolation by acid guanidine isothiocyanate-phenol-chloroform extraction
3. Isolation and characterization of cDNA clones from human placenta coding for phospholipase A2
4. 2 gene expression in human hepatoma cells by mediators of the acute phase response
5. Amplification and detection of lentiviral DNA inside cells
6. Feasibility of archival non-buffered formalin-fixed and paraffin-embedded tissues for PCR amplification: An analysis of resected gastric carcinoma
7. Evaluation of the effects of DNase treatment on signal specificity in RT-PCR and in situ RT-PCR
8. An improved method for chromosome-specific labeling of alpha satellite DNA in situ by using denatured double-stranded DNA probes as primers in a primed in situ labeling (PRINS) procedure
9. Evaluation of methods for hepatitis C virus detection in archival liver biopsies: Comparison of histology, immunohistochemistry, in-situ hybridization, reverse transcriptase polymerase chain reaction (RT-PCR) and in-situ RT-PCR
10. Target and signal amplification: Approaches to increase the sensitivity of in situ hybridisation
11. Comparison of indirect and direct in-situ polymerase chain reaction in cell preparations and tissue sections
12. 2 in human atherosclerotic plaques
13. 2-specific mRNA in cytokine-stimulated HepG2 cells by in situ-RT-nested PCR
14. 2 in relation to the presence of microbial agents, macrophage infiltrates, and transcripts of proinflammatory cytokines in human aortic tissues
15. Detection of genomic DNA with a high sensitivity in tissue sections using a two step cycling in situ PCR procedure
16. Importance of different variables for optimizing in situ detection of PCT-amplified DNA
17. Detection of rare RNA sequences by single-enzyme in situ reverse transcription-polymerase chain reaction. High-resolution analyses of interleukin-6 mRNA in paraffin sections of lymph nodes
18. Nonspecific amplification in in-situ PCR by direct incorporation of reporter molecules