Botulinum neurotoxin types B and E: Purification, limited proteolysis by endoproteinase Glu-C and pepsin, and comparison of their identified cleaved sites relative to the three-dimensional structure of type A neurotoxin

Toxicon

Volumn 39, Issue 10, 2001, Pages 1515-1531

  Prabakaran, S ^a   Tepp, W ^a   DasGupta, B R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Amino acid residues exposed; Botulinum neurotoxin; Crystal structure; Folding; Fragments sequences; Proteolysis

Indexed keywords

ASPARAGINE; BOTULINUM TOXIN; BOTULINUM TOXIN A; BOTULINUM TOXIN B; BOTULINUM TOXIN E; LEUCINE; PEPSIN A; PROTEINASE; PROTEINASE GLUTAMATE C; UNCLASSIFIED DRUG;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CLOSTRIDIUM BOTULINUM; CONTROLLED STUDY; CRYSTALLIZATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN DOMAIN; PROTEIN ISOLATION; PROTEIN PURIFICATION; TOXIN STRUCTURE;

AMINO ACID SEQUENCE; BINDING SITES; BOTULINUM TOXIN TYPE A; BOTULINUM TOXINS; CHROMATOGRAPHY, AGAROSE; CHROMATOGRAPHY, DEAE-CELLULOSE; CLOSTRIDIUM BOTULINUM; CRYSTALLOGRAPHY, X-RAY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; NEUROMUSCULAR AGENTS; PEPSIN A; PEPTIDE FRAGMENTS; PROTEIN CONFORMATION; SEQUENCE ALIGNMENT; SERINE ENDOPEPTIDASES;

EID: 0034908275     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0041-0101(01)00124-6     Document Type: Article

References (52)

1. Chains and fragments of tetanus toxin, and their contribution to toxicity
2. Covalent structure of botulinum neurotoxin type E. Location of sulfhydryl groups, and disulfide bridges and identification of C-termini of light and heavy chains
3. Covalent structure of botulinum neurotoxin type B; location of sulfhydryl groups and disulfide bridge and identification of C-termini of light and heavy chains
4. Status of Cys residues in the covalent structure of botulinum neurotoxin types A, B, and E
5. Preclinical update on BoTox (botulinum toxin type A)-purified neurotoxin complex relative to other botulinum neurotoxin preparations
6. Role of the heavy and light chains of botulinum neurotoxin in neuromuscular paralysis
7. Botulinum neurotoxin type A. Limited proteolysis by endoproteinase Glu-C and alpha-chymotrypsin enhanced following reduction; identification of the cleaved sites and fragments
8. Proteolytic cleavage of tetanus toxin increases activity
9. The N-terminal half of the heavy chain of botulinum type A neurotoxin forms channels in planar phospholipid bilayers
10. Structure and biological activity of botulinum neurotoxin
11. Structure of botulinum neurotoxin, its functional domains, and perspectives on the crystalline type A toxin
12. C. botulinum neurotoxin types A and E: Isolated light chain breaks down into two fragments. Comparison of their amino acid sequences with tetanus toxin
13. Purification and amino acid composition of type E botulinum neurotoxin
14. Purification and amino acid composition of type A botulinum neurotoxin
15. A common subunit structure in Clostridium botulinum type A, B and E toxins
16. Molecular forms of neurotoxins in proteolytic Clostridium botulinum Type B cultures
17. Amino acid composition of Clostridium botulinum type B neurotoxin
18. Nicking of single chain Clostridium botulinum type A neurotoxin by an endogenous protease
19. Tyrosine phosphorylation modulates the activity of clostridial neurotoxins
20. Botulinum neurotoxin type E fragmented with endoproteinase Lys-C reveals the site trypsin nicks and homology with tetanus neurotoxin
21. Pepsin fragmentation of botulinum type E neurotoxin: Isolation and characterization of 112, 48, 46 and 16 kDa fragments
22. Botulinum type A neurotoxin digested with pepsin yields 132, 97, 72, 45, 42 and 18 kDa fragments
23. Simplified purification method for Clostridium botulinum type E toxin
24. Cocrystal structure of synaptobrevin-II bound to botulinum neurotoxin type B at 2.0 Å resolution
25. C-terminal half of tetanus toxin fragment C is sufficient for neuronal binding and interaction with a putative protein receptor
26. How botulinum and tetanus neurotoxin block neurotransmitter release
27. Purification and some properties of Clostridium botulinum type-E toxin
28. Induction of an immune response by oral administration of recombinant botulinum toxin
29. Antigenicities of fragments of Clostridium botulinum type B derivative toxin
30. Purification and some properties of progenitor toxins of Clostridium botulinum type B
31. Activation of Clostridium botulinum type B and E derivative toxins with lysine-specific proteases
32. Covalent structure of botulinum neurotoxin type A: Location of sulfhydrl groups, and disulfide bridges and identification of C-termini of light and heavy chains
33. Sequence homology and structural analysis of the clostridial neurotoxins
34. Crystal structure of botulinum neurotoxin type A and implications for toxicity
35. A single mutation in the recombinant light chain of tetanus toxin abolishes its proteolytic activity and removes the toxicity seen after reconstitution with native heavy chain
36. Probing the mechanistic role of glutamate residue in the zinc-binding motif of type A botulinum neurotoxin light chain
37. Sequence from picomole quantities of proteins electroblotted onto polyvinylidene difluoride membranes
38. Molecular genetics of clostridial neurotoxins
39. Identification of an ion channel-forming motif in the primary structure of tetanus and botulinum neurotoxins
40. Structure and function of tetanus and botulinum neurotoxins
41. Formation of ion channels in lipid bilayers by a peptide with the predicted transmembrane sequence of botulinum neurotoxin A
42. Dichain structure of botulinum neurotoxin: Identification of cleavage sites in types C, D, and F neurotoxin molecules
43. Separation, purification, partial characterization and comparison of the heavy and light chains of botulinum neurotoxin types A, B, and E
44. Partial amino acid sequences of the heavy and light chains of botulinum neurotoxin type E
45. 2-terminus of the heavy subunit of Clostridium botulinum type A neurotoxin forms channels in lipid vesicles
46. 1, and E produce proteins with or without hemagglutinating activity: Do they share common amino acid sequences and genes?
47. Crystallization and preliminary X-ray analysis of botulinum neurotoxin type A
48. Structural analysis of the catalytic and binding sites of Clostridium botulinum neurotoxin B
49. Molecular cloning of the Clostridium botulinum structural gene encoding the type B neurotoxin and determination of its entire nucleotide sequence
50. Fast protein liquid chromatography of botulinum neurotoxin types A, B and E
51. Effect of tetranitromethane on the biological activities of botulinum neurotoxin types A, B and E