Covalent structure of botulinum neurotoxin type E: Location of sulfhydryl groups, and disulfide bridges and identification of C-termini of light and heavy chains

Journal of Protein Chemistry

Volumn 16, Issue 8, 1997, Pages 787-799

  Antharavally, Babu S ^a   DasGupta, Bibhuti R ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Botulinum neurotoxin; C termini; CNBr, pepsin, clostripain fragmentation; Disulfide; HPLC; SDS PAGE separation; Sulfhydryl

Indexed keywords

BOTULINUM TOXIN; DISULFIDE; NEUROTOXIN; THIOL GROUP;

AMINO ACID SEQUENCE; ARTICLE; CLOSTRIDIUM BOTULINUM; COVALENT BOND; DISULFIDE BOND; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN PROCESSING; STRUCTURE ANALYSIS; TOXIN ANALYSIS; TOXIN STRUCTURE;

AMINO ACID SEQUENCE; BOTULINUM TOXINS; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; SEQUENCE ANALYSIS; SULFHYDRYL COMPOUNDS;

EID: 0030853242     PISSN: 02778033     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1026367917639     Document Type: Article

References (37)

1. Allen, G. (1989). In Laboratory Techniques in Biochemistry and Molecular Biology (Burdon, R. H., and Knippenberg, P. H., eds.) Amsterdam, Elsevier, pp. 56-61.
2. Antharavally, B. S., Tepp, W., and DasGupta, B. R. J. Protein Chem. (in press).
3. Bonventre, P. F., and Kempe, L. L. (1959). J. Bacteriol. 78, 892-893.
4. Bonventre, P. F., and Kempe, L. L. (1960). J. Bacteriol. 78, 24-32.
5. Charbonneau, H. (1989). In Practical Guide to Protein and Peptide Purification for Microsequencing (Matsudaira, P. T., ed.), Academic Press, New York, pp. 17-29.
6. DasGupta, B. R. (1971). J. Bacteriol. 108, 1051-1057.
7. DasGupta, B. R. (1990). J. Physiol. (Paris) 84, 220-228.
8. DasGupta, B. R., and Dekleva, M. L. (1990). Biochimie 72, 661-664.
9. DasGupta, B. R., and Sugiyama, H. (1972a). Biochim. Biophys. Acta 268, 719-729.
10. DasGupta, B. R., and Sugiyama, H. (1972b). Biochem. Biophys. Res. Commun. 48, 108-112.
11. DasGupta, B. R., and Sugiyama, H. (1972c). Infect. Immun. 6, 587-590.
12. Dekleva, M. L., and DasGupta, B. R. (1989). Biochem. Biophys. Res. Commun. 162, 767-772.
13. Dixon, H. B. F., and Perham, R. N. (1968). Biochem. J. 109, 312-314.
14. Duff, J. T., Wright, G. G., and Yarinsky, A. (1956). J. Bacteriol. 72, 455-460.
15. Dreyfuss, G., Adam, S. A., and Choi, Y. D. (1984). Mol. Cell. Biol. 4, 415-423.
16. Eklund, M. W., and Dowell, V. R., Jr., eds. (1987). Avian Botulism: An International Perspective. Charles C. Thomas, Springfield, Illinois.
17. Fontana, A., and Gross, E. (1986). In Practical Protein Chemistry - A Handbook (Darbre, A., ed.), Wiley-Interscience, New York, pp. 67-120.
18. Gimenez, J. A., and DasGupta, B. R. (1990). Biochimie 72, 213-217.
19. Gimenez, J. A., and DasGupta, B. R. (1992). J. Protein Chem. 11, 255-263.
20. Gimenez, J. A., and Sugiyama, H. (1987). Appl. Environ. Microbiol. 53, 2827-2830.
21. Gimenez, J. A., Lomneth, R., and DasGupta, B. R. (1994). Toxicon 32, 1137-1146.
22. Heimsch, R. C. (1973). Ph.D. Thesis, University of Wisconsin-Madison.
23. Hunkapiller, M. W., Lujan, E., Ostrunder, F., and Hood, L. E. (1983). Meth. Enzymol. 91, 227-236.
24. Kitamura, M., Sakaguchi, S., and Sakaguchi, G. (1969). J. Bacteriol. 98, 1173-1178.
25. Krieglstein, K. G., DasGupta, B. R., and Henschen, A. H. (1994). J. Protein Chem. 13, 49-57.
26. Matsudaira, p. (1987). J. Biol. Chem. 262, 10035-10038.
27. Minton, N. P. (1995). In Clostridial Neurotoxins (Montecucco, C., ed.), Springer, Berlin, pp. 161-194.
28. Montecucco, C., and Schiavo, G. (1995). Q. Rev. Biophys. 28, 423-472.
29. Poulet, S., Hauser, D., Quanz, M., Niemann, H., and Popoff, M. R. (1992). Biochem. Biophys. Res. Commun. 183, 107-113.
30. Sathyamoorthy, V., and DasGupta, B. R. (1985a). Biochem. Biophys. Res. Commun. 127, 768-772.
31. Sathyamoorthy, V., and DasGupta, B. R. (1985b). J. Biol. Chem. 260, 10461-10466.
32. Sathyamoorthy, V., Dasgupta, B. R., Foley, J., and Niece, R. L. (1988). Arch. Biochem. Biophys. 266, 142-151.
33. Schagger, H., and Jagow, G. V. (1987). Anal. Biochem. 166, 368-379.
34. Schmidt, J. J., Sathyamoorthy, V., and DasGupta, B. R. (1985). Arch. Biochem. Biophys. 238, 544-548.
35. Tacket, C. O., and Rogawski, M. A. (1989). In Botulinum Neurotoxin and Tetanus Toxin (Simpson, L. L., ed.), Academic Press, New York, pp. 351-378.
36. Villa, S., De Fazio, G., and Canosii, U. (1989). Anal. Biochem. 177, 161-164.
37. Whelan, S. M., Elmore, M. J., Bodsworth, N. J., Atkinson, T., and Minton, N. P. (1992). Eur. J. Biochem. 204, 657-667.