A direct streptavidin-binding assay does not accurately quantitate biotin in human urine

Journal of Nutrition

Volumn 131, Issue 8, 2001, Pages 2208-2214

  Mock, D M ^a   Nyalala, J O ^a   Raguseo, R M ^a  

^a UNIVERSITY OF ARKANSAS FOR MEDICAL SCIENCES   (United States)

Author keywords

Avidin; Biotin; Europium; Metabolites; Streptavidin; Urine

Indexed keywords

AVIDIN; BIOTIN; EUROPIUM; STREPTAVIDIN;

ARTICLE; CLINICAL ARTICLE; CORRELATION FUNCTION; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; METABOLITE; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN URINE LEVEL; QUANTITATIVE ASSAY; URINALYSIS;

SABA;

EID: 0034900082     PISSN: 00223166     EISSN: None     Source Type: Journal    
DOI: 10.1093/jn/131.8.2208     Document Type: Article

References (18)

1. Increased urinary excretion of 3-hydroxyisovaleric acid and decreased urinary excretion of biotin are sensitive early indicators of decreased status in experimental biotin deficiency
2. Determinations of biotin in biological fluids
3. Biotin
4. Biotin and biotin analogs in human urine: Biotin accounts for only half of the total
5. Bacterial degradation of biotin VI. Isolation and Identification of β-hydroxy and β-keto compounds
6. Identification of biotin sulfone, bisnorbiotin methyl ketone, and tetranorbiotin-I-sulfoxide in human urine
7. Advanced analysis of biotin metabolites in body fluids allows a more accurate measurement of biotin bioavailability and metabolism in humans
8. The biotin-(strept)avidin system: Principles and applications in biotechnology
9. Urinary biotin analogs increase in humans during chronic supplementation: The analogs are biotin metabolites
10. Avidin 3. The nature of the biotin-binding site
11. Introduction to symposium: Nutrition, biochemistry and molecular biology of biotin
12. Biotin status: Which are valid indicators and how do we know?
13. Profound biotinidase deficiency in two asymptomatic adults
14. Biotin deficiency results from long-term therapy with anticonvulsants
15. The metabolite profile of radioisotope labeled biotin in rats indicates that biotin metabolism is similar to that in humans
16. Conflicting indicators of biotin status from a cross-sectional study of normal pregnancy
17. Biotin status assessed longitudinally in pregnant women
18. Biotin catabolism is accelerated in adults receiving long-term therapy with anticonvulsants