Control of the efficiency of agonist-induced information transfer and stability of the ternary complex containing the δ opioid receptor and the α subunit of Gi1 by mutation of a receptor/G protein contact interface

Neuropharmacology

Volumn 41, Issue 3, 2001, Pages 321-330

  Moon, H E ^a   Bahia, D S ^a   Cavalli, A ^a   Hoffmann, M ^a   Milligan, G ^a  

^a UNIVERSITY OF GLASGOW   (United Kingdom)

Author keywords

Fusion proteins; G protein; Opiate; Ternary complex

Indexed keywords

AMINO ACID; CYSTEINE; DELTA OPIATE RECEPTOR; ENKEPHALIN[2 DEXTRO ALANINE 5 DEXTRO LEUCINE]; GLYCINE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE DIPHOSPHATE; HYBRID PROTEIN; ISOLEUCINE; NALTRINDOLE; OCTANOL; PROTEIN SUBUNIT; TRITIUM;

ALPHA CHAIN; ARTICLE; BINDING AFFINITY; BINDING KINETICS; CONTROLLED STUDY; DISSOCIATION; HUMAN; HYDROLYSIS; MEMBRANE BINDING; MUTATION; PRIORITY JOURNAL; PROTEIN STABILITY;

CELL MEMBRANE; CELLS, CULTURED; ENKEPHALIN, LEUCINE-2-ALANINE; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; HETEROTRIMERIC GTP-BINDING PROTEINS; HUMANS; KINETICS; NALTREXONE; NARCOTIC ANTAGONISTS; OLIGONUCLEOTIDES, ANTISENSE; RECEPTORS, SIGMA; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 0034891058     PISSN: 00283908     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0028-3908(01)00076-4     Document Type: Article

References (30)

1. 2A-adrenoceptor
2. Inhibition of receptor/G protein coupling by suramin analogues
3. Differential effects of suramin on the coupling of receptors to individual species of pertussis toxin-sensitive guanine nucleotide binding proteins
4. The heterotrimeric G protein G alpha i2 mediates lysophosphatidic acid-stimulated induction of the c-fos gene in mouse fibroblasts
5. Analysis of agonist function at fusion proteins between the IP prostanoid receptor and cognate, unnatural and chimaeric G proteins
6. G protein antagonists
7. G protein-coupled receptors. II. Mechanism of agonist activation
8. Receptor-stimulated hydrolysis of guanosine 5′-triphosphate in membrane preparations
9. G proteins: Transducers of receptor-generated signals
10. The many faces of G protein signaling
11. i1α
12. i1α
13. The role of members of the pertussis toxin-sensitive family of G proteins in coupling receptors to the activation of the G protein-gated inwardly rectifying potassium channel
14. i2
15. Techniques used in the identification and analysis of function of pertussis toxin-sensitive guanine nucleotide binding proteins
16. Specificity and functional applications of antipeptide antisera which identify G-protein α subunits
17. Insights into ligand pharmacology using receptor-G protein fusion proteins
18. Chimaeric Gα proteins: Their potential use in drug discovery
19. Structural basis of G protein-coupled receptor function
20. sα has properties of a constitutively active receptor
21. Effects of guanine, inosine, and xanthine nucleotides on beta(2)-adrenergic receptor/G(s) interactions: Evidence for multiple receptor conformations
22. GPCR-Galpha fusion proteins: Molecular analysis of receptor-G-protein coupling
23. The D2 dopamine receptor isoforms signal through distinct Gi alpha proteins to inhibit adenylyl cyclase. A study with site-directed mutant Gi alpha proteins
24. Identification of receptor contact site involved in receptor-G protein coupling
25. Kinetics of ternary complex formation with fusion proteins composed of the A1-adenosine receptor and G protein α-subunits
26. Molecular basis of receptor/G-protein-coupling selectivity
27. i1α fusion protein
28. i-family G-proteins: Studies with pertussis toxin-resistant G-protein mutants
29. The rat D4 dopamine receptor couples to cone transducin (Galpha t2) to inhibit forskolin-stimulated cAMP accumulation
30. Conformational changes at the carboxyl terminus of Galpha occur during G protein activation