Ionization properties of titratable groups in ribonuclease T1: I. pKa values in the native state determined by two-dimensional heteronuclear NMR spectroscopy

European Biophysics Journal

Volumn 30, Issue 3, 2001, Pages 186-197

  Spitzner, N ^a   Lohr F ^a   Pfeiffer, S ^a   Koumanov, A ^a   Karshikoff, A ^a   Ruterjans H ^a  

^a GOETHE UNIVERSITY   (Germany)

Author keywords

Histidine tautomer formation; Nuclear magnetic resonance; PH titration; PKa values; Ribonuclease T1

Indexed keywords

CARBON 13; CARBOXYLIC ACID DERIVATIVE; HISTIDINE; IMIDAZOLE DERIVATIVE; NITROGEN 15; PROTEIN D76; PROTEIN E28; PROTEIN E82; PROTEIN H27; PROTEIN H40; PROTEIN R77; PROTEIN SUBUNIT; RIBONUCLEASE T1; UNCLASSIFIED DRUG;

AMINO ACID ANALYSIS; ARTICLE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; ENZYME ACTIVE SITE; ENZYME STRUCTURE; IONIZATION; MATHEMATICAL ANALYSIS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; PH; PROTEIN INTERACTION; PROTON TRANSPORT; RESIDUE ANALYSIS; TITRIMETRY;

ESCHERICHIA COLI; HISTIDINE; HYDROGEN-ION CONCENTRATION; IONS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, MOLECULAR; MODELS, STATISTICAL; PROTEIN CONFORMATION; PROTONS; RIBONUCLEASE T1; SPECTROPHOTOMETRY;

EID: 0034886835     PISSN: 01757571     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002490100138     Document Type: Article

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