Increased calpain I-mediated proteolysis, and preferential loss of dephosphorylated NF200, following traumatic spinal cord injury

Neuroscience

Volumn 91, Issue 2, 1999, Pages 733-744

  Schumacher, P A ^a   Eubanks, J H ^a   Fehlings, M G ^a  

^a TORONTO WESTERN HOSPITAL   (Canada)

Author keywords

Calpain; Nerve tissue proteins; Neurofilament; Spinal cord injury

Indexed keywords

CALPAIN; NEUROFILAMENT PROTEIN;

ANIMAL TISSUE; ARTICLE; CYTOSKELETON; DEPHOSPHORYLATION; IMMUNOBLOTTING; IMMUNOHISTOCHEMISTRY; NONHUMAN; PATHOPHYSIOLOGY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN PHOSPHORYLATION; RAT; SPINAL CORD INJURY; THORACIC SPINAL CORD; TIME;

ANIMALS; AXONS; CALPAIN; FEMALE; IMMUNOHISTOCHEMISTRY; NEUROFILAMENT PROTEINS; PHOSPHORYLATION; RATS; RATS, WISTAR; SPECTRIN; SPINAL CORD; SPINAL CORD INJURIES; TIME FACTORS;

EID: 0032909284     PISSN: 03064522     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0306-4522(98)00552-1     Document Type: Article

References (49)

1. Agrawal S.K., Fehlings M.G. Mechanisms of secondary injury to spinal cord axons in vitro: role of Na+, Na(+)-K(+)-ATPase, the Na(+)-H+ exchanger, and the Na(+)-Ca2+ exchanger. J. Neurosci. 16:1996;545-552.
2. Agrawal S.K., Fehlings M.G. Role of NMDA and non-NMDA ionotropic glutamate receptors in traumatic spinal cord axonal injury. J. Neurosci. 17:1997;1055-1063.
3. Arai A., Kessler M., Lee K., Lynch G. Calpain inhibitors improve the recovery of synaptic transmission from hypoxia in hippocampal slices. Brain Res. 532(1-2):1990;63-68.
4. Banik N.L., Hogan E.L., Hsu C.Y. The multimolecular cascade of spinal cord injury. Studies on prostanoids, calcium, and proteinases. Neurochem. Path. 7:1987;57-77.
5. Banik N.L., Hogan E.L., Powers J.M., Smith K.P. Proteolytic enzymes in experimental spinal cord injury. J. Neurol. Sci. 73:1986;245-256.
6. Banik N.L., Hogan E.L., Powers J.M., Whetstine L.J. Degradation of cytoskeletal proteins in experimental spinal cord injury. Neurochem. Res. 7:1982;1465-1475.
7. 2-induced myelopathy in rat spinal cord. Central nerv. Syst. Trauma. 1:1984;131-137.
8. Bartus R.T., Baker K.L., Heiser A.D., Sawyer S.D., Dean R.L., Elliott P.J., Straub J.A. Postischemic administration of AK275, a calpain inhibitor, provides substantial protection against focal ischemic brain damage. J. cerebr. Blood Flow Metab. 14:1994;537-544.
9. Bartus R.T., Hayward N.J., Elliott P.J., Sawyer S.D., Baker K.L., Dean R.L., Akiyama A., Straub J.A., Harbeson S.L., Li Z. Calpain inhibitor AK295 protects neurons from focal brain ischemia. Effects of postocclusion intra-arterial administration. Stroke. 25:1994;2265-2270.
10. Bi X., Tocco G., Baudry M. Calpain-mediated regulation of AMPA receptors in adult rat brain. NeuroReport. 6:1994;61-64.
11. Caner H., Collins J.L., Harris S.M., Kassell N.F., Lee K.S. Attenuation of AMPA-induced neurotoxicity by a calpain inhibitor. Brain Res. 607:1993;354-356.
12. Carden M.J., Trojanowski J.Q., Schlaepfer W.W., Lee V.M. Two-stage expression of neurofilament polypeptides during rat neurogenesis with early establishment of adult phosphorylation patterns. J. Neurosci. 7:1987;3489-3504.
13. Croall D.E., DeMartino G.N. Calcium-activated neutral protease (calpain) system: structure, function, and regulation. Physiol. Rev. 71:1991;813-847.
14. Fehlings M.G., Agrawal S. Role of sodium in the pathophysiology of secondary spinal cord injury. Spine. 20:1995;2187-2191.
15. Fehlings M.G., Nashmi R. Assessment of axonal dysfunction in an in vitro model of acute compressive injury to adult rat spinal cord axons. Brain Res. 677:1995;291-299.
16. Geisler N., Vandekerckhove J., Weber K. Location and sequence characterization of the major phosphorylation sites of the high molecular mass neurofilament proteins M and H. Fedn Eur. biochem. Socs Lett. 221:1987;403-407.
17. Goll D.E., Dayton W.R., Singh I., Robson R.M. Studies of the alpha-actinin/actin interaction in the Z-disk by using calpain. J. biol. Chem. 266:1991;8501-8510.
18. Greenwood J.A., Troncoso J.C., Costello A.C., Johnson G.V. Phosphorylation modulates calpain-mediated proteolysis and calmodulin binding of the 200-kDa and 160-kDa neurofilament proteins. J. Neurochem. 61:1993;191-199.
19. Hamakubo T., Kannagi R., Murachi T., Matus A. Distribution of calpains I and II in rat brain. J. Neurosci. 6:1986;3103-3111.
20. 2-induced myelopathy. Neurosci. Lett. 49:1984;279-283.
21. Happel R.D., Smith K.P., Banik N.L., Powers J.M., Hogan E.L., Balentine J.D. Ca2+-accumulation in experimental spinal cord trauma. Brain Res. 211:1981;476-479.
22. Hayes R.L., Yang K., Whitson J.S., Postmantur R. Cytoskeletal derangements following central nervous system injury: modulation by neurotrophic gene transfection. J. Neurotrauma. 12:1995;933-941.
23. Hoffman P.N., Cleveland D.W., Griffin J.W., Landes P.W., Cowan N.J., Price D.L. Neurofilament gene expression: a major determinant of axonal caliber. Proc. natn. Acad. Sci. U.S.A. 84:1987;3472-3476.
24. Hoffman P.N., Thompson G.W., Griffin J.W., Price D.L. Changes in neurofilament transport coincide temporally with alterations in the caliber of axons in regenerating motor fibers. J. Cell Biol. 101:1985;1332-1340.
25. Kaku Y., Yonekawa Y., Tsukahara T., Ogata N., Kimura T., Taniguchi T. Alterations of a 200 kDa neurofilament in the rat hippocampus after forebrain ischemia. J. cerebr. Blood Flow Metab. 13:1993;402-408.
26. Kampfl A., Posmantur R., Nixon R., Grynspan F., Zhao X., Liu S.J., Newcomb J.K., Clifton G.L., Hayes R.L. mu-Calpain activation and calpain-mediated cytoskeletal proteolysis following traumatic brain injury. J. Neurochem. 67:1996;1575-1583.
27. Kishimoto A. Limited proteolysis of protein kinase C by calpain, its possible implication. Adv. Second Mess. Phosphoprot. Res. 24:1990;472-477.
28. Li Z., Hogan E.L., Banik N.L. Role of calpain in spinal cord injury: increased μ-calpain immunoreactivity in spinal cord after compression injury in the rat. Neurochem. Int. 27:1995;425-432.
29. Li Z., Hogan E.L., Banik N.L. Role of calpain in spinal cord injury: increased calpain immunoreactivity in rat spinal cord after impact trauma. Neurochem. Res. 21:1996;441-448.
30. Lynch G., Baudry M. Brain spectrin, calpain and long-term changes in synaptic efficacy. Brain Res. Bull. 18:1987;809-815.
31. Melloni E., Pontremoli S. The calpains. Trends Neurosci. 12:1989;438-444.
32. Nakamura M., Araki M., Oguro K., Masuzawa T. Differential distribution of 68 Kd and 200 Kd neurofilament proteins in the gerbil hippocampus and their early distributional changes following transient forebrain ischemia. Expl Brain Res. 89:1992;31-39.
33. Nixon R.A., Sihag R.K. Neurofilament phosphorylation: a new look at regulation and function. Trends Neurosci. 14:1991;501-506.
34. Pant H.C. Dephosphorylation of neurofilament proteins enhances their susceptibility to degradation by calpain. Biochem. J. 256:1988;665-668.
35. Perlmutter L.S., Siman R., Gall C., Seubert P., Baudry M., Lynch G. The ultrastructural localization of calcium-activated protease "calpain" in rat brain. Synapse. 2:1988;79-88.
36. Peterson G.L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analyt. Biochem. 83:1977;346-356.
37. Posmantur R., Hayes R.L., Dixon C.E., Taft W.C. Neurofilament 68 and neurofilament 200 protein levels decrease after traumatic brain injury. J. Neurotrauma. 11:1994;533-545.
38. Posmantur R., Kampfl A., Siman R., Liu J., Zhao X., Clifton G.L., Hayes R.L. A calpain inhibitor attenuates cortical cytoskeletal protein loss after experimental traumatic brain injury in the rat. Neuroscience. 77:1997;875-888.
39. Rivlin A.S., Tator C.H. Effect of vasodilators and myelotomy on recovery after acute spinal cord injury in rats. J. Neurosurg. 50:1979;349-352.
40. Roberts-Lewis J.M., Savage M.J., Marcy V.R., Pinsker L.R., Siman R. Immunolocalization of calpain I-mediated spectrin degradation to vulnerable neurons in the ischemic gerbil brain. J. Neurosci. 14:1994;3934-3944.
41. Saatman K.E., Bozyczko-Coyne D., Marcy V., Siman R., McIntosh T.K. Prolonged calpain-mediated spectrin breakdown occurs regionally following experimental brain injury in the rat. J. Neuropath. exp. Neurol. 55:1996;850-860.
42. Saatman K.E., Murai H., Bartus R.T., Smith D.H., Hayward N.J., Perri B.R., McIntosh T.K. Calpain inhibitor AK295 attenuates motor and cognitive deficits following experimental brain injury in the rat. Proc. natn. Acad. Sci. U.S.A. 93:1996;3428-3433.
43. Saido T.C., Sorimachi H., Suzuki K. Calpain: new perspectives in molecular diversity and physiological-pathological involvement. Fedn Am. Soc. exp. Biol. J. 8:1994;814-822.
44. Siman R., Gall C., Perlmutter L.S., Christian C., Baudry M., Lynch G. Distribution of calpain I, an enzyme associated with degenerative activity, in rat brain. Brain Res. 347:1985;399-403.
45. Siman R., Noszek J.C. Excitatory amino acids activate calpain I and induce structural protein breakdown in vivo. Neuron. 1:1988;279-287.
46. Springer J.E., Azbill R.D., Kennedy S.E., George J., Geddes J.W. Rapid calpain I activation and cytoskeletal degradation following traumatic spinal cord injury: attenuation with riluzole pretreatment. J. Neurochem. 69:1997;1592-1600.
47. Suzuki K., Saido T.C., Hirai S. Modulation of cellular signals by calpain. Ann. N. Y. Acad. Sci. 674:1992;218-227.
48. Tator C.H., Fehlings M.G. Review of the secondary injury theory of acute spinal cord trauma with emphasis on vascular mechanisms. J. Neurosurg. 75:1991;15-26.
49. Yakovlev A.G., Faden A.I. Molecular biology of CNS injury. J. Neurotrauma. 12:1995;767-777.