Arginine-140 and isoleucine-141 determine the 17β-estradiol-binding specificity of the sex-steroid-binding protein (SBP, or SHBG) of human plasma

Protein Science

Volumn 10, Issue 9, 2001, Pages 1811-1821

  Petra, Philip H ^a   Adman, Elinor T ^b   Orr, William R ^a   Woodcock, Katherine T ^a   Groff, Christine ^a   Sui, Li Ming ^c  

^a UNIVERSITY OF WASHINGTON SCHOOL OF MEDICINE   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

^c ELI LILLY AND COMPANY   (United States)

Author keywords

17 estradiol; Dihydrotestosterone; Sex hormone binding globulin, SHBG; Sex steroid binding protein, SBP; Steroid binding site; Testosterone

Indexed keywords

AMINO ACID; ANDROSTANOLONE; ARGININE; ESTRADIOL; ISOLEUCINE; SEX HORMONE BINDING PROTEIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; DISSOCIATION CONSTANT; HUMAN; MOLECULAR MODEL; NONHUMAN; PLASMA; PRIORITY JOURNAL;

AMINO ACID SEQUENCE; ANIMALS; ARGININE; BINDING SITES; ESTRADIOL; HUMANS; ISOLEUCINE; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN BINDING; PROTEIN ISOFORMS; RABBITS; SEQUENCE HOMOLOGY, AMINO ACID; SEX HORMONE-BINDING GLOBULIN; SUBSTRATE SPECIFICITY; THERMODYNAMICS;

ORYCTOLAGUS CUNICULUS;

EID: 0034848127     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.02301     Document Type: Article

References (38)

1. Testosterone and androstenedione blood production rates in normal women and women with idiopathic hirsutism or polycystic ovaries
2. Secondary structure and shape of plasma sex steroid-binding protein. Comparison with domain G of laminin results in a structural model of plasma sex steroid-binding protein
3. The protein data bank
4. The receptor-mediated action of Sex Steroid-binding Protein (SBP, SHBG): Accumulation of cAMP in MCF-7 cells under SBP and estradiol treatment
5. A structural snapshot of an intermediate on the streptavidin-biotin dissociation pathway
6. Characterization of Met-139 as the photolabeled amino acid residue in the steroid binding site of sex hormone binding globulin using Δ6 derivatives of either testosterone or estradiol as unsubstituted photoaffinity labeling reagents
7. The amino acid sequence of the sex steroid-binding protein of rabbit serum
8. Crystal structure of human sex hormone-binding globulin: Steroid transport by a laminin G-like domain
9. Mammalian expression of the Human Sex Steroid-Binding Protein of Plasma (SBP or SHBG) and Testis (ABP). Characterization of the recombinant protein
10. Structure, function, and regulation of androgen-binding protein/sex hormone-binding globulin
11. 6-Testosterone photoreagent
12. Tolerance of 5-fluorodeoxyuridine resistant human thymidylate synthases to alterations in active site residues
13. Rabbit sex hormone binding globulin: Primary structure, tissue expression, and structure/function analyses by expression in Escherichia coli
14. Purification and physico-chemical characterization of the sex steroid-binding protein of rabbit serum. Comparison with the human protein
15. Biologically active steroids activate receptor-bound human sex hormone-binding globulin to cause LNCaP cells to accumulate adenosine 3′,5′-monophosphate
16. The steroid-binding properties of pure SBP: Effects of long-term storage on the equilibrium constant and stoichiometry of binding of the human, monkey, and rabbit proteins
17. Identification of Lysine-134 in the steroid binding site of the sex steroid binding protein of human plasma
18. Selective delivery of sex steroid hormones to tissues in vivo by albumin and sex hormone-binding globulin
19. The serum sex steroid-binding protein. Purification, characterization, and immunological properties of the human and rabbit proteins
20. The plasma sex steroid-binding protein (SBP or SHBG). A critical review of recent developments on the structure, molecular biology, and function
21. Direct influence of the sex steroid-binding protein (SBP) of plasma on the metabolic clearance rate of testosterone
22. Molecular characterization of the sex steroid-binding protein (SBP) of plasma. Re-examination of rabbit SBP and comparison with the human, macaque, and baboon proteins
23. Affinity labeling, molecular cloning, and comparative amino acid sequence analyses of sex steroid-binding protein of plasma. A multidisciplinary approach for understanding steroid-protein interaction and its physiological role
24. Complete Enzymatic Deglycosylation of Native Sex Steroid-Binding Protein (SBP or SHBG) of Human and Rabbit Plasma. Effect on the Steroid-Binding Activity
25. The sex steroid binding protein (SBP or SHBG) of human plasma: Identification of Tyr-57 and Met-104 in the steroid binding site
26. Direct effect of Plasma Sex Hormone Binding Globulin (SHBG) on the metabolic clearance rate of 17β-estradiol in the primate
27. Demonstration and partial characterization of a rabbit serum protein which binds testosterone and dihydrotestosterone
28. Testosterone-estradiol-binding globulin of human plasma: Denaturation and protection
29. Observation and quantitation of metal binding sites in the Sex Steroid-binding Protein of human and rabbit sera using the luminescent probe, terbium
30. A recognition system for sex-hormone-binding protein-estradiol complex in human decidual endometrium plasma membranes
31. Localization of the steroid-binding site of the human sex steroid-binding protein of plasma (SBP or SHBG) by site-directed mutagenesis
32. Direct evidence for the localization of the steroid-binding site of the plasma sex steroid-binding protein (SBP or SHBG) at the interface between the subunits
33. Heterologous expression of wild type and deglycosylated human plasma sex steroid-binding protein (SBP or SHBG) in the yeast, Pichia pastoris. Characterization of the recombinant proteins
34. Capacity of the testosterone binding globulin in human plasma and influence of specific binding of testosterone on its metabolic clearance rate
35. Amino acid sequence of the sex steroid-binding protein (SBP) of human blood plasma
36. New force field for molecular mechanical simulation of nucleic acids and proteins