Rapid isolation, characterization, and glycan analysis of Cup a 1, the major allergen of Arizona cypress (Cupressus arizonica) pollen

Allergy: European Journal of Allergy and Clinical Immunology

Volumn 56, Issue 10, 2001, Pages 978-984

  Alisi, C ^a   Afferni, C ^a   Iacovacci, P ^a   Barletta, B ^a   Tinghino, R ^a   Butteroni, C ^a   Puggioni, E M R ^a   Wilson, I B H ^b   Federico, R ^c   Schinina M E ^d   Ariano, R ^e   Di Felice, G ^e   Pini, Carlo ^a,e  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b UNIVERSITY OF NATURAL RESOURCES AND LIFE SCIENCES   (Austria)

^c ROMA TRE UNIVERSITY   (Italy)

^d SAPIENZA UNIVERSITY OF ROME   (Italy)

^e USL 1 Imperiese   (Italy)

Author keywords

Cupressus arizonica allergens; Glycan structures; MALDI TOF; Purification

Indexed keywords

ALLERGEN; CUP A 1 ALLERGEN; CUPRESSUS ARIZONICA EXTRACT; GLYCAN; IMMUNOGLOBULIN E; N ACETYLGLUCOSAMINE; OLIGOSACCHARIDE; PLANT EXTRACT; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANALYTIC METHOD; ARTICLE; CHEMICAL STRUCTURE; CHROMATOGRAPHY; CROSS REACTION; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMUNOBLOTTING; IMMUNOCHEMISTRY; IMMUNOREACTIVITY; ISOELECTRIC POINT; LECTIN BINDING; MASS SPECTROMETRY; MOLECULAR WEIGHT; NONHUMAN; POLLEN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN ISOLATION; PROTEIN PURIFICATION; SEQUENCE HOMOLOGY;

EID: 0034841009     PISSN: 01054538     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1398-9995.2001.103125.x     Document Type: Article

References (26)

1. Increase of respiratory allergy to pollens of cypresses in the south of France
2. Cypress pollen from Arizona and allergic respiratory sensitization in Italy
3. Cypress allergy: An underestimated pollinosis. A four year prospective clinical study
4. Assessment of skin prick test and serum specific IgE detection in the diagnosis of Cupressaceae pollinosis
5. Allergens of Arizona cypress (Cupressus arizonica) pollen: Characterization of the pollen extract and identification of the allergenic components
6. Arizona cypress (Cupressus arizonica) pollen allergens. Identification of cross-reactive periodate-resistant and -sensitive epitopes with monoclonal antibodies
7. Role of carbohydrate moieties in IgE binding to allergenic components of Cupressus arizonica pollen extract
8. Cross-reactivity between Cupressus arizonica and Cupressus sempervirens pollen extract
9. Juniperus oxicedrus: A new allergenic pollen from the Cupressaceae family
10. N-Glycan analysis by matrix-assisted laser desorption/ionisation mass spectrometry of electrophoretically separated nonmammalian proteins: Application to peanut allergen Ara h 1 and olive pollen allergen Ol'e e 1
11. 4-(N-acetyl-beta-glucosaminyl) asparagine amidase A and its N-glycans
12. Colorimetric method for determination of sugars and related substances
13. A rapid and sensitive method for quantitation of micrograms of proteins utilizing the principle of protein-dye binding
14. Isolation and characterization of the mountain cedar (Juniperus ashei) pollen major allergen, Jun a 1
15. Purification, characterization and molecular cloning of Cha o 1, a major allergen of Chamaecyparis obtusa (Japanese cypress) pollen
16. Cloning and sequencing of cDNA coding for Cry j 1, a major allergen of Japanese cedar pollen
17. Structures and contribution to the antigenicity of oligosaccharides of Japanese cedar (Cryptomeria japonica) pollen allergen Cry j I: Relationship between the structures and antigenic epitopes of plant N-linked complex-type glycans
18. a epitope are expressed at the surface of plant cells
19. a carbohydrate motif in a secretory peroxidase from a plant cell suspension culture (Vaccinium myrtillus L.)
20. a, core α1,3-fucose and xylose substitutions
21. Structural analysis of N-glycans from allergenic grass ragweed and tree pollens: Core alpha 1,3-linked fucose and xylose present in all pollens examined
22. Molecular cloning of major allergen from Cupressus arizonica pollen: Cup a 1
23. Cry j 1, a major allergen of Japanese cedar pollen, has pectate lyase enzyme activity
24. The parallel β helix of pectate lyase C: Something to sneeze at
25. Epitopes on Cry j I and Cry j II for the human IgE antibodies cross reactive between Cupressus sempervirens and Cryptomeria japonica pollen
26. A monoclonal antibody specific for a carbohydrate epitope recognizes an IgE-binding determinant shared by taxonomically unrelated allergenic pollens