An improved method for DNA extraction from paraffin sections

Pathology Research and Practice

Volumn 197, Issue 9, 2001, Pages 635-642

  Man, Yan Gao ^a   Moinfar, Farid ^a   Bratthauer, Gary L ^a   Kuhls, Elizabeth A ^a   Tavassoli, Fattaneh A ^a  

^a ARMED FORCES INSTITUTE OF PATHOLOGY   (United States)

Author keywords

Comparative genetic analysis; DNA extraction; Histotechniques; Paraffin sections

Indexed keywords

DNA; ENZYME; HEMATOXYLIN; PARAFFIN; XYLENE;

ARTICLE; CELL COUNT; CONTROLLED STUDY; EXTRACTION; HUMAN; HUMAN TISSUE; INCUBATION TIME; METHODOLOGY; MICROSCOPY; POLYMERASE CHAIN REACTION; STAINING; TEMPERATURE; TISSUE PREPARATION; TISSUE SECTION;

EID: 0034837317     PISSN: 03440338     EISSN: None     Source Type: Journal    
DOI: 10.1078/0344-0338-00138     Document Type: Article

References (26)

1. Methylation of Hpa II and Hha 1 sites near the polymorphic CAG repeat in the human androgen receptor gene correlates with X-chromosome inactivation
2. Effects of processing at 45 °C on staining
3. Comparison of histologic stains for use in PCR analysis of microdissected, paraffin-embedded tissues
4. Monoclonal antibody labeling of mononuclear cells surface antigens in formaldehyde-fixed paraffin-embedded cutaneous tissue
5. Inhibitors of the polymerase chain reaction in Papanicolaou stain: Removal with a simple destaining procedure
6. PCR amplification from paraffin-embedded tissues: Recommendations on fixatives for long-term storage and prospective studies
7. Isolation of nucleic acid from paraffin embedded tissue for PCR amplification and sequencing of TcR V beta genes
8. Comparison of loss of heterozygosity in primary and recurrent ductal carcinoma in situ of the breast
9. LOH at 16p13 is a novel chromosomal alteration detected in benign and malignant microdissected papillary neoplasm of the breast
10. Loss of heteroxygosity is detected at chromosomes 1p35-36(NB), 3p25(VHL), 16p13 (TSC2/PKD1), and 17p13 (TP53) in microdissected apocrine carcinomas of the breast
11. Effect of microwave oven heating times on androgen receptor antigen retrieval from paraffin-embedded prostatic adenocarcinoma
12. Multiple use of slab gels in sequencing apparatus for separation of PCR products
13. A fast way to prepare paraffin sections for HE and immunostaining
14. Five useful approaches for generating more valid gel images of LOH and clonality analysis with an automated 377 DNA sequencer
15. Detection of telomerase activity in microdissected breast lesions
16. A simple epitope retrieval method without the use of microwave oven or enzyme digestion
17. Detailed RT-ISPCR protocol for preserving morphology and confining PCR products in routinely processed paraffin sections
18. Endocrine tumors of the cervix: Morphologic assessment, expression of human papillomavirus, and evaluation for loss of heterozygosity on 1p, 3p, 11q, and 17p
19. Concurrent and independent genetic alterations in the stromal and epithelial cells of mammary carcinoma: Implications for tumorigenesis
20. Genetic abnormality in ductal intraepithelial neoplasia of the flat type (Clinging carcinoma) of the breast
21. Leydig cells in Sertoli-Leydig cell tumors have a reactive rather than a neoplastic molecular profile
22. Fixation, processing, and immunochemical reagent effects on preservation of T-lymphocyte surface membrane antigens in paraffin-embedded tissue
23. Standardization of the Papanicolaou stain. 1. A comparison of five nuclear stains
24. Fixed archival tissue: Purify DNA and primers for good PCR yield
25. Identical clonality of both components of mammary carcinoma with differential loss of heterozygosity